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53results about How to "Reduce the risk of false positives" patented technology

Urea concentration fault detection method and device, control equipment and storage medium

The invention provides a urea concentration fault detection method and device, control equipment and a storage medium. The method comprises steps of judging whether a selective catalytic reduction system has the fault of low nitrogen oxide conversion efficiency or not; if yes, spraying urea according to a preset first urea ammonia nitrogen ratio, and storing first conversion efficiency of nitric oxide of the selective catalytic reduction system; spraying urea according to a preset second urea ammonia nitrogen ratio, and storing second conversion efficiency of nitrogen oxide of the selective catalytic reduction system; judging whether the first conversion efficiency and the second conversion efficiency are lower than a first preset threshold value or not; if so, determining that the fault is caused by low urea concentration. According to the embodiment of the invention, when the fault that the NOx conversion efficiency of a SCR system is low is detected, the SCR system fault caused by low urea concentration is accurately judged by comparing the distribution conditions of the NOx conversion efficiency when urea is injected at different ammonia-nitrogen ratios, and the risk of false alarm is reduced.
Owner:WEICHAI POWER CO LTD

Method for judging fault of rotation speed sensor of double clutch transmission output shaft

The invention provides a method for judging faults of a rotation speed sensor of a double clutch transmission output shaft. The method comprises the following steps: judging whether the difference between arithmetic mean values of rotation speed signals of the double clutch transmission output shaft and rotation speed signals of driving wheels of an ABS is within an acceptable deviation range; when the difference is within the acceptable deviation range, judging whether omega inp is equal to omega outp*igear, wherein omega inp is a rotation speed signal of the double clutch transmission output shaft, omega outp is a rotation speed signal of the double clutch transmission output shaft, and igear is the total transmission ratio of a shift when a double clutch transmission is in stable transmission; when omega inp is not equal to omega outp*igear, judging whether omega engine is equal to omega outp*igear, wherein omega engine is a rotation speed signal of an engine; when omega engine is not equal to omega outp*igear, transmitting a signal for indicating that the rotation speed sensor of the double clutch transmission output shaft has faults. By adopting the method, the false alarm rate of the rotation speed sensor of the double clutch transmission output shaft is greatly reduced.
Owner:ANHUI JIANGHUAI AUTOMOBILE GRP CORP LTD

Primer pair used for detecting CYP2C19 genotypes, fluorescent probe, reagent kit and method

The invention discloses a reagent kit used for detecting CYP2C19 genotypes. Allelic genotypes of CYP2C19*2(681G / A) and CYP2C19*3(636G / A) of a CYP2C19 gene are detected by designing a specific primer pair and a fluorescent probe. Asymmetric PCR and the fluorescent probe melting curve analysis technology are combined, different genotypes are judged through melting peaks of specific temperatures, detection of six genotypes related to CYP2C19*2 and CYP2C19*3 can be achieved in one-tube PCR, subsequent processing is not needed, the possibility of reactant pollution is avoided to the largest extent, operation is easy, consumed time is short, specificity is good, accuracy is high, and results are easy to interpret.
Owner:智海生物工程(北京)股份有限公司

Primer pair, fluorescence probe and kit for detecting polymorphism of MTHFR gene

The invention provides a primer pair, specific oligonucleotide fluorescence probe and kit for detecting the nucleotide polymorphism of a C677T locus of an MTHFR gene. The new specific primer pair and the corresponding fluorescence probe are designed, PCR amplification is performed by adopting an asymmetric PCR technology, melting curve analysis is performed after PCR amplification is finished, and a genetype is judged through the melting peak at specific temperature. According to the kit, the MTHFR gene can be amplified in a highly specific mode, detection of three kinds of genetypes of the C677T locus in a single-pipe PCR system can be completed, the detection specificity is high, the result is easy to judge and read, the operating steps are simple, the detection cost is low, the cycle is short, and the efficiency is high.
Owner:智海生物工程(北京)股份有限公司

POTDR (polarization optical time domain reflectometer) based optical fiber intrusion recognition algorithm

The invention belongs to the technical field of optical fiber sensing and discloses a POTDR (polarization optical time domain reflectometer) based optical fiber intrusion recognition algorithm. The algorithm includes reading initial data of 2N cycles which are acquired by an optical fiber vibration sensor at an acquisition terminal and then denoising; dividing an array X acquired after denoising and solving the difference value deltaX; dividing the difference value deltaX into multiple equal-length window arrays to form an array Y; calculating forth-order central distance of the window arrays of the array Y, comparing the forth-order central distance with the set threshold D, calibrating the above-threshold data Yaj of the array Y, and inputting the subscript aj to a coordinate array A; positioning an alarm point according to the minimum value amin of the array A, and repeating the step to perform the next recognition if no above-threshold array exists. Specifically, the arrangement mode of the initial data is consistent with the arrangement mode of sampling points of the optical fiber sensor, the subscripts of the window arrays are coordinates of the corresponding sampling points; the coordinates of the sampling points represent optical length of the sampling points in the optical fiber. With the window arrays, reliability and positioning accuracy of the intrusion recognition algorithm are improved.
Owner:武汉世纪金桥安全技术有限公司

Method for detecting sheep FecB gene polymorphism by using micro-fluidic SNP chip

The invention provides a method for detecting sheep FecB gene polymorphism by using a micro-fluidic SNP chip. The method is capable of detecting loci A and G at high throughput and the result is accurate. Compared with other methods for high throughput detection of an FecB gene by using a Taqman MGB probe, the method provided by the invention has the advantages that samples capable of being detected every time are relatively flexible in quantity, the consumption of a reagent is low and the cost is significantly reduced. Nucleotide sequences of used primer and probe are as shown in SEQ ID NO.1-4, so that the specificity is high, the interference of other genes is avoided, the false positive risk caused by PCR product pollution is reduced and result interpretation is relatively intuitive. High throughput detection on the SNP loci of the FecB gene can be achieved, and the method has potential application value in large-scale molecular breeding of sheep.
Owner:INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI +1

Kit for detecting human PEAR1 gene polymorphism and application thereof

The invention relates to a kit for detecting human PEAR1 gene polymorphism and an application thereof. The kit includes the following substances: a specific primer and a specific fluorescent probe for detecting an rs2768759 locus on PEAR1 gene, a specific primer and a specific fluorescent probe for detecting an rs12041331 locus on the PEAR1 gene, a Taq DNA polymerase, dNTP mixed liquid, a MgCl2 solution, a fluorescent quantitative PCR reaction buffer liquid and deionized water. The kit overcomes the defects in various gene mutation detection methods, is high in detection accuracy, is simple and convenient, is short in detection time and is simple in result analysis. The kit is suitable for clinical laboratories and can perform qualitative detection to the two polymorphic sites, the rs2768759 and the rs12041331, on the PEAR1 gene, wherein a PCR fluorescent amplification reaction is carried out according to the SNP locus. A result can be determined just on the basis of whether two different fluorescent curves are positive or not without manual error, so that the kit is improved in efficiency and reduced in detection cost. The kit is free of DNA extraction, wherein a suspension liquid after cell pyrolysis is added to the reaction system to complete amplification.
Owner:南京仁天生物科技有限公司

Method for analyzing phenolic compounds in water

The invention discloses a method for analyzing phenolic compounds in water, used for qualitatively or quantitatively analyzing the phenolic compounds in a water sample. The method comprises the following steps of 1, producing a standard curve; 2, preparing sample liquid to be detected: preprocessing a surface water sample to be detected to acquire the sample liquid to be detected; 3, detecting andanalyzing the sample liquid to be detected by using gas chromatography-low energy electron ionization quadrupole time-of-flight mass spectrometry (GC-le EI-QTOF); and 4, reviewing the sample that isdetected to be positive, namely that includes the phenolic compounds, by using gas chromatography-triple quadrupole mass spectrometry (GC-QQQ). The method provided by the invention is high in resolution, high in sensitivity, and capable of accurately determining the nature of a target, and excluding interferent of which a mass-to-charge ratio is close to that of the target.
Owner:HARBIN INST OF TECH

Hepatitis C virus recombination protein and gene sequence

The invention discloses hepatitis C virus recombination protein, characterized in that: the hepatitis C virus recombination protein is fusion protein formed by fusing hepatitis C virus main antigenic determinant and hepatitis C virus core protein single chain antibody, and the amino acid sequence is represented as SEQ ID No.1. According to the invention, gene engineering recombination technology is utilized, the hepatitis C virus main antigenic determinant-core protein single chain antibody fusion protein is expressed in escherichia coli system, and the advantages of short production period, high yield and low cost are achieved. The recombination protein can be used as a part of an immunodiagnostic kit for hepatitis C virus.
Owner:杭州博林生物技术有限公司

Unmanned aerial vehicle obstacle-avoiding system and unmanned aerial vehicle obstacle-avoiding method

The invention discloses an unmanned aerial vehicle obstacle-avoiding system and an unmanned aerial vehicle obstacle-avoiding method. The system comprises a radar device and an unmanned aerial vehiclemotion information transmission device, wherein the radar device is connected with the unmanned aerial vehicle motion information transmission device by a flight control system in an unmanned aerial vehicle, the unmanned aerial vehicle motion information transmission device is used for acquiring motion information during the running process of the unmanned aerial vehicle, the motion information issent to the radar device by the flight control system, and the radar device is used for adjusting a target detection tracing strategy according to the received motion information in real time and outputting the detected target information to the flight control system to control the unmanned aerial vehicle to fly. The system has the advantages of simple and compact structure, low cost, high obstacle-avoiding accuracy, safety and accuracy and the like.
Owner:成都纳雷科技有限公司

Self-adaptive thermal management system for dealing with lithium battery parking thermal runaway

The invention provides a self-adaptive thermal management system for dealing with lithium battery parking thermal runaway, a composite separator plate is arranged between lithium battery monomers, the self-adaptive thermal management system comprises a phase change material, the phase change material stores heat during normal work, and the phase change material has poor thermal conductivity, and a metal cavity enhances thermal conductivity; during shutdown thermal runaway, the phase change material around the lithium battery is difficult to meet the heat storage requirement, is melted and transfers heat to the shape memory alloy driving mechanism in the metal cavity, and the shape memory alloy extends and pushes the metal sliding block to move, so that the purpose of isolating the thermal runaway battery is achieved; secondly, during parking, the alarm device cannot be started, and, at the moment, the metal sliding block serves as a heat source of the thermoelectric power generation piece to generate current so as to start the alarm device; finally, the temperature of the battery box is further increased, so that the medium-temperature phase-change material at the top end is melted, and the dry powder extinguishing agent sealed at the top end is released to extinguish fire. According to the invention, early warning, isolation and fire extinguishing are integrated, and the problem of thermal runaway of the lithium battery during parking can be adaptively solved.
Owner:HARBIN INST OF TECH

Text data risk identification method and server

The invention discloses a text data risk identification method and a server. The method comprises the steps of collecting first text data of a target application embedded in an application platform; obtaining a legal text template of the target application; based on the legal text template of the target application, deleting target text content associated with the legal text template from the first text data to obtain second text data of the target application; and inputting the second text data of the target application into a preset risk identification model for risk identification.
Owner:ADVANCED NEW TECH CO LTD

Evaluation method of detection result accuracy of rapid organophosphorus and carbamate pesticide residue detector

The invention discloses an evaluation method of detection result accuracy of a rapid organophosphorus and carbamate pesticide residue detector. The evaluation method comprises the following steps of: (1) preparing a standard reference sample containing the effective components of organophosphorus and an carbamic acid ester; (2) determining an enzyme inhibition ratio reference value and standard deviation of the standard reference sample; (3) measuring the enzyme inhibition ratio of the standard reference sample by using the rapid organophosphorus and carbamate pesticide residue detector; (4) comparing an enzyme inhibition ratio detection value and the enzyme inhibition ratio reference value; and (5) obtaining an evaluation result. The evaluation method provided by the invention can effectively evaluate the detection result accuracy of the rapid organophosphorus and carbamate pesticide residue detector.
Owner:苏州慧康电子信息科技有限公司

Establishment of methodology for detecting genes affecting efficacy of lipid-lowering drugs by TaqMan-MGB probe technique

The invention discloses a method for detecting genes affecting the efficacy of lipid-lowering drugs by combining a TaqMan-MGB probe and a real-time fluorescence quantitative PCR technique. The polymorphism of the genes affecting the efficacy of the lipid-lowering drugs in samples to be detected is detected, six SNP loci can be detected simultaneously, used primers and the TaqMan-MGB probe have high specificity, the risk of false positive caused by PCR product contamination can be reduced, the operation is simple and the result is accurate and reliable. According to the method, functional meaningful mutations such as SLCO1B1 c.521T>C (rs4149056), SLCO1B1 c.388A>G(rs2302683), SLCO1B1 c.-910G>A(rs4149015), ApoE c.388T> C(rs429358), ApoE c.526C>T(rs7412) and NPC1L1 c.-18C>A(rs41279633) are detected to assist clinicians to select appropriate drugs and doses or avoid occurring of drug interactions, the best therapeutic effect for patients is achieved, and thus the purpose of real ''individualized medication'' is achieved.
Owner:江苏百世诺医疗科技有限公司

Detection kit and detection method for pathogen nucleic acid under airtight condition

The invention discloses a detection kit and a detection method for pathogenic nucleic acid under an airtight condition, and belongs to the technical field of nucleic acid extraction. The detection kit comprises a sealing box body and a sealing cover, a first detergent chamber, a cracking buffering agent chamber, a second detergent chamber, an elution buffering agent chamber, a purification membrane chamber, a waste liquid chamber, a reaction detection chamber and a sealing membrane which is arranged on the channel and can be opened are sequentially arranged in the sealing box body from top to bottom, wherein the first detergent chamber, the cracking buffering agent chamber, the second detergent chamber, the elution buffering agent chamber, the purification membrane chamber, the waste liquid chamber and the reaction detection chamber are connected through the channel; and the detection method comprises the steps that the cracking buffering agent chamber, the first detergent chamber, the second detergent chamber and the elution buffering agent chamber are isolated from the channel through sealing films, after corresponding steps are completed, laser breaks through the sealing films, and liquid in corresponding cavities is centrifugally driven to flow into the channel. Preloaded reagents are isolated through the sealing films, laser drilling and centrifugal force driving are completely achieved in a non-contact mode, and on the basis of the chip structure design of fluid properties, pathogenic nucleic acid extraction and real-time fluorescent PCR amplification detection of the detection kit under the airtight and full-closed condition are achieved.
Owner:北京芯源视界生物科技有限公司

Method for determining VOCs in packaging material through purging and trapping-gas chromatography-mass spectrometry

The invention relates to a method for determining VOCs in a packaging material through purging and trapping-gas chromatography-mass spectrometry. The method is characterized by comprising the steps ofsample preparation, purging and trapping and sample introduction, chromatography-mass spectrometry combined determination, qualitative confirmation, quantitative analysis and calculation result analysis. The method is scientific and reasonable in structural design, and the content of 25 kinds of volatile organic pollutants (VOCs) in the packaging material and products thereof can be determined bythe method for determining the VOCs in the packaging material through the purge-trap-gas chromatography-mass spectrometry; through the purging and trapping and sampling mode, extraction and concentration by using an organic solvent are avoided, so that the interference of an external solvent is reduced; compared with a headspace mode, the purging and trapping mode needs smaller area of the sample, and the sensitivity is higher; a gas chromatograph-mass spectrometer is used for detection, so that the qualitative ability of the method is enhanced, and the false positive risk is reduced; and patent test types are many, including alcohols, esters, ketones, benzenes, alkenes, halogenated hydrocarbons and the like, and the test requirements of VOCs in packaging materials at present are basically met.
Owner:CHINA PACKAGING RES & TEST CENT

Kit for detecting polymorphism of human ABCB1 gene and application thereof

The invention relates to a kit for detecting polymorphism of a human ABCB1 gene and an application thereof. Substances in the kit comprise a specific primer and a specific fluorescent probe for detecting an rs1045642 SNP locus on the ABCB1 gene, Taq DNA polymerase, a dNTP mixed liquid, an MgCl2 solution, a fluorescence quantitative PCR reaction buffer solution and deionized water. The defects of various genetic mutation detection methods are overcome. The kit has the advantages of high detection accuracy, simple and convenient detection, short detection time, and simple result analysis, is suitable for use in clinical laboratories, can perform qualitative detection on a CYP2C19 gene polymorphism locus, performs PCR fluorescent amplification reaction according to the SNP locus, can judge a result just according to a condition whether two different fluorescence curves are started or no, cannot produce artificial errors, has low false positive and false negative rates, reduces detection costs, has no need for DNA extraction, can complete amplification only with cracking of complete blood cells, and improves the detection accuracy.
Owner:南京仁天生物科技有限公司

Performance monitoring and fault diagnosis method for high-pressure EGR cooler

The invention discloses a performance monitoring and fault diagnosis method for a high-pressure EGR cooler. The performance monitoring and fault diagnosis method comprises the following steps that S1, when all diagnosis conditions are met, a signal delay counter is added, and after the diagnosis conditions are met and lasts for set time, an EGR cooler performance diagnosis function is started; S2, the cooling efficiency of the EGR cooler is monitored and diagnosed according to the ratio of the EGR cooler front and back temperature drop calculated based on the measured value of an EGR cooler outlet temperature sensor to the EGR cooler temperature drop calculated based on an EGR cooler outlet temperature model; and S3, if the performance monitoring index of the EGR cooler is lower than a diagnosis threshold value, it is judged that the performance of the EGR cooler fails, and the low cooling efficiency fault of the EGR cooler is reported, and if the performance monitoring index of the EGR cooler is higher than the diagnosis threshold value, it is judged that the performance of the EGR cooler is normal. According to the performance monitoring and fault diagnosis method, the reliability is high, the fault diagnosis robustness is enhanced, and the false alarm risk is reduced.
Owner:DONGFENG AUTOMOBILE COMPANY

SNP molecular marker related to lambing number of sheep single birth, primer probe set, kit, detection method and application

The invention provides an SNP molecular marker related to lambing number of a sheep single birth, a primer probe set, a kit, a detection method and application, and belongs to the technical field of sheep SNP molecular markers. The SNP molecular marker is located on a No.40857869bp site on a No.1 chromosome of sheep, the site is located in an LEPR gene, C / T base mutation exists in the site, the gene type of the LEPR gene is CC or CT or TT, the lambing number of the sheep single birth of the gene type CC is lager than that of the gene type TT, and the lambing number of the sheep single birth ofthe gene type TT is larger than that of the gene type CT; a sheep gene group sequence information which SNP molecular marker information is based on is Oar_v3.1. The SNP molecular marker is related to the lambing number of the sheep single birth remarkably, and according to the SNP molecular marker, the lambing capacity of the sheep single birth can be judged. In practical application, ewes in aCC type shall be selected as reserved ewes, and the heterozygous ewes and the ewes in a CT type are removed during selection.
Owner:INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI

Detection kit for intelligence gene

The present invention discloses a detection kit for intelligence genes. The detection kit comprises a PCR reaction reagent, and specific primers and fluorescent probes for gene locus SNP detection, wherein the gene loci comprise JMJD1C gene locus rs7923609 and LRRC14 gene locus rs2721173. According to the present invention, the detection of different genotypes of the two loci can be completed in the single-tube PCR system through the detection kit of the present invention, such that the operation steps are less, the time consumption is short, the tube opening operation is not required, the possibility of the PCR product pollution is reduced, the specificity of the detection is high, and the result is easy to interpret.
Owner:才赋基因科技(北京)有限公司

Cable insulation online monitoring method based on KPCA-NSVDD

The invention discloses a cable insulation online monitoring method based on KPCA-NSVDD. The cable insulation on-line monitoring method comprises the following steps: firstly, acquiring cable insulation historical data including a small number of fault samples as an original training data set; secondly, in order to solve the problem that high-dimensional data is difficult to process, using kernel principal component analysis (KPCA) for carrying out dimension reduction processing on the data; and then establishing an NSVDD (support vector data description with fault samples) training model by using the training sample, and reflecting the real-time state of cable insulation by using the absolute distance between the real-time sample and the constructed NSVDD hypersphere center. The sliding window mechanism is introduced, the training model can be updated in real time, and the anomaly recognition accuracy is improved. The established model can accurately grasp the behavior rule of normal work of the unit, and the operation state of the overhead line is monitored on line through the established model, so that timely early warning of abnormity is realized.
Owner:CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Kit for detecting human COX-2 gene polymorphism and application thereof

The invention relates to a kit for detecting human COX-2 gene polymorphism and an application thereof. The kit includes the following substances: a specific primer and a specific fluorescent probe for detecting an rs20417 SNP locus on COX-2 gene, a Taq DNA polymerase, a dNTP mixed liquid, a MgCl2 solution, a fluorescent quantitative PCR reaction buffer liquid and deionized water. The kit overcomes the defects in various gene mutation detection methods, is high in detection accuracy, is simple and convenient, is short in detection time and is simple in result analysis. The kit is suitable for clinical laboratories and can perform qualitative detection to COX-2 gene polymorphic sites, wherein a PCR fluorescent amplification reaction is carried out according to the SNP locus. A result can be determined just on the basis of whether two different fluorescent curves are positive or not, so that the kit is free of manual error, is low in false positive and false negative rate, and is improved in efficiency and reduced in detection cost.
Owner:南京仁天生物科技有限公司

Control method for output signal of automobile urea quality sensor

The invention relates to an automobile urea quality sensor output signal control method, which comprises the following steps that a urea quality sensor is arranged in a urea box, and a certain amount of urea solution is filled in the urea box; a power supply of the urea quality sensor is switched on, and the urea quality sensor works normally and records voltage energy signals converted from five continuous ultrasonic echo signals; if the number of the energy signals higher than the threshold value line in the five signals recorded continuously is larger than or equal to 3, it is judged that the output signals of the sensor are reliable, and the ECU outputs the actually-calculated urea concentration value, and if the number of the energy signals higher than the threshold value line in the five signals recorded continuously is smaller than or equal to 2, it is judged that the output signals of the sensor are not reliable, and the sensor outputs invalid signal codes; and the electronic control unit ECU outputs the reliable signal which is output last time as a replacement signal. According to the method, the sensor signal judgment logic is optimized, so that the sensor output value is more reliable, and the system robustness and the customer driving experience are improved.
Owner:JIANGLING MOTORS

Method of using taqman probe process for screening of TP53 susceptibility gene, and probe and primer used in the method

The invention provides a method of using taqman probe process for screening of TP53 susceptibility gene. The method includes the steps of: 1) extracting genome from normal people in a manner of oral cavity swab; 2) designing TP53 primers and probes; 3) performing PCR reaction to the genome with the TP53 primers and probes; 4) after the PCR reaction is finished, performing fluorescence reading andgenetic typing; 5) performing comprehensive evaluation to tumor susceptibility of individual by combining the results detected by the four probes. The taqman primers and probes have excellent genetictyping effect and can very accurately determine the genotype of the individual.
Owner:美因健康科技(北京)有限公司

Backscattering maze

The invention provides a backscattering maze comprising a light shielding wall and a tube seat. The tube base is provided with a transmitting unit and a receiving unit, and the transmitting unit and the receiving unit are symmetrically arranged along the central axis of the tube seat. An angle beta is formed by the central axis of the tube seat and the central axis of the maze, and beta is largerthan 0 degree and is smaller than 90 degrees. The central optical axis of the transmitting unit and the central optical axis of the receiving unit meet on the central axis of the maze. Since a certainangle is formed by the central axis of the tube seat and the central axis of the maze in the invention, light emitted by the transmitting unit can be reflected on the light shielding wall for many times due to the existence of the angle, and the reflected light finally enters into the receiving unit. As the number of reflections increases, the light energy gradually decays, and thus the background value of the maze is reduced. When the background value is reduced, the difference between the background value and a fire alarm value is increased, and the risk of false alarm is reduced.
Owner:深圳市高新投三江电子股份有限公司

Human mthfr gene polymorphism detection kit and method based on taqman-mgb probe

The invention belongs to the field of genetic gene detection, and in particular relates to a kit and a method for detecting the gene polymorphism of human MTHFR (methylene tetrahydrofolate reductase) based on a Taqman-MGB (Minor Groove Binder) probe. By adopting the kit and method, two SNP (single nucleotide polymorphism) sites namely C677t and A1298C can be detected at the same time, and the results are easy to judge and read. The MGB probe has a nonluminous 3'-end quenching group and a relatively low background, is more sensitive to detection of a single base mutation, and can judge and read different genotypes only according to a Ct value, and the result can be judged and read more easily compared with that of conventional methods of Taqman probe detection and HRM (high resolution melting) detection. The probe price is relatively low, the detection cost is relatively low, a polymorphism site only needs one-tube qPCR (quantitative polymerase chain reaction) detection, and the operation is simple. Moreover, the method disclosed by the invention does not need subsequent analysis of PCR products, so that while the detection cost is saved, the detection circle is greatly shortened, the detection efficiency is improved and the risk of false positive caused by PCR product pollution is reduced.
Owner:HENAN UNIV OF SCI & TECH

Microfluidic chip for capturing and/or counting cells, its preparation method and application

The invention is applicable to the technical field of biological detection, and provides a microfluidic chip for capturing and / or counting cells and its preparation method and application. The microfluidic chip includes: a first base layer; an antibody-modified inverse opal photonic crystal structure , set on the first base layer, which includes antibody modification layer and doped with Yb 3+ and Er 3+ The yttrium vanadate inverse opal structure layer; the second base layer is set on the side of the first base layer close to the antibody-modified inverse opal photonic crystal structure; the microfluidic cavity is set between the first base layer and the second base layer. The invention controls the photonic bandgap by regulating the minimum repeating unit size of the photonic crystal, and makes double-layered Yb-doped materials with different apertures and different bandgaps. 3+ and Er 3+ The inverse opal structure of yttrium vanadate can significantly enhance the upconversion green light emission through the bandgap effect of photonic crystals, which solves the problem of insufficient fluorescence intensity caused by the low quantum efficiency of the existing upconversion luminescence process.
Owner:JILIN UNIV

A kit for rapidly detecting human cyp2c19 gene polymorphism and its application method

ActiveCN104212904BAvoid the disadvantages of difficult judgment of resultsNo human errorMicrobiological testing/measurementMedicineHigh flux
The invention belongs to the gene detecting technology in clinical detecting technologies of the bio-medical field and particularly relates to a kit for quickly detecting polymorphism of a human CYP2C19 gene and a using method of the kit. The kit comprises three pairs of primers, three probes, six PNA (pentose nucleic acid) sequences, a pair of beta-Actin interior label primers and an interior label probe, and further comprises a PCR (polymerase chain reaction) buffer solution of Mg<2+>, a dNTP mixture, Taq enzyme and ddH2O. The kit can be utilized to carry out qualitative detection on a polymorphic site of the CYP2C19 gene and carry out two-tube PNA-PCR fluorescent amplified reaction according to the SNP site and can carry out result judgment according to a condition whether a two-tube fluorescence curve is started or not without producing personal errors, so that a false positive rate and a false negative rate are low. And moreover, the kit can be used to realize high-flux detection easily and is capable of clinical use.
Owner:WUHAN HEALTHCHART BIOLOGICAL TECH

A kind of acrylonitrile-styrene copolymer material and preparation method thereof

The invention discloses an acrylonitrile-styrene copolymer material for promoting ultrasonic attenuation and a preparation method thereof, belonging to the technical field of polymer materials. The acrylonitrile-styrene copolymer material for promoting ultrasonic attenuation according to the present invention comprises the following components in parts by weight: 40-90 parts of acrylonitrile-styrene resin and 10-50 parts of ultrasonic attenuation agent; the ultrasonic attenuation agent comprises acrylonitrile -Styrene-Acrylate Copolymer, Acrylonitrile-Styrene-Butadiene Copolymer, Acrylonitrile-Styrene-Ethylene-propylene Rubber Copolymer, Acrylate-Styrene-Butadiene Copolymer, Styrene-Ethylene- At least one of butene-styrene copolymers. The acrylonitrile-styrene copolymer material prepared by the formula of the present invention can make the radar wave signal of false alarm to be lost in the transmission process, and can be used to prepare the grille, bumper and car logo of the vehicle equipped with the ultrasonic radar wave detector and other auto parts.
Owner:KINGFA SCI & TECH CO LTD
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