53results about How to "Reduce the risk of false positives" patented technology

The invention provides a urea concentration fault detection method and device, control equipment and a storage medium. The method comprises steps of judging whether a selective catalytic reduction system has the fault of low nitrogen oxide conversion efficiency or not; if yes, spraying urea according to a preset first urea ammonia nitrogen ratio, and storing first conversion efficiency of nitric oxide of the selective catalytic reduction system; spraying urea according to a preset second urea ammonia nitrogen ratio, and storing second conversion efficiency of nitrogen oxide of the selective catalytic reduction system; judging whether the first conversion efficiency and the second conversion efficiency are lower than a first preset threshold value or not; if so, determining that the fault is caused by low urea concentration. According to the embodiment of the invention, when the fault that the NOx conversion efficiency of a SCR system is low is detected, the SCR system fault caused by low urea concentration is accurately judged by comparing the distribution conditions of the NOx conversion efficiency when urea is injected at different ammonia-nitrogen ratios, and the risk of false alarm is reduced.

The invention provides a primer pair, specific oligonucleotide fluorescence probe and kit for detecting the nucleotide polymorphism of a C677T locus of an MTHFR gene. The new specific primer pair and the corresponding fluorescence probe are designed, PCR amplification is performed by adopting an asymmetric PCR technology, melting curve analysis is performed after PCR amplification is finished, and a genetype is judged through the melting peak at specific temperature. According to the kit, the MTHFR gene can be amplified in a highly specific mode, detection of three kinds of genetypes of the C677T locus in a single-pipe PCR system can be completed, the detection specificity is high, the result is easy to judge and read, the operating steps are simple, the detection cost is low, the cycle is short, and the efficiency is high.

The invention relates to a kit for detecting human PEAR1 gene polymorphism and an application thereof. The kit includes the following substances: a specific primer and a specific fluorescent probe for detecting an rs2768759 locus on PEAR1 gene, a specific primer and a specific fluorescent probe for detecting an rs12041331 locus on the PEAR1 gene, a Taq DNA polymerase, dNTP mixed liquid, a MgCl2 solution, a fluorescent quantitative PCR reaction buffer liquid and deionized water. The kit overcomes the defects in various gene mutation detection methods, is high in detection accuracy, is simple and convenient, is short in detection time and is simple in result analysis. The kit is suitable for clinical laboratories and can perform qualitative detection to the two polymorphic sites, the rs2768759 and the rs12041331, on the PEAR1 gene, wherein a PCR fluorescent amplification reaction is carried out according to the SNP locus. A result can be determined just on the basis of whether two different fluorescent curves are positive or not without manual error, so that the kit is improved in efficiency and reduced in detection cost. The kit is free of DNA extraction, wherein a suspension liquid after cell pyrolysis is added to the reaction system to complete amplification.

The invention provides a self-adaptive thermal management system for dealing with lithium battery parking thermal runaway, a composite separator plate is arranged between lithium battery monomers, the self-adaptive thermal management system comprises a phase change material, the phase change material stores heat during normal work, and the phase change material has poor thermal conductivity, and a metal cavity enhances thermal conductivity; during shutdown thermal runaway, the phase change material around the lithium battery is difficult to meet the heat storage requirement, is melted and transfers heat to the shape memory alloy driving mechanism in the metal cavity, and the shape memory alloy extends and pushes the metal sliding block to move, so that the purpose of isolating the thermal runaway battery is achieved; secondly, during parking, the alarm device cannot be started, and, at the moment, the metal sliding block serves as a heat source of the thermoelectric power generation piece to generate current so as to start the alarm device; finally, the temperature of the battery box is further increased, so that the medium-temperature phase-change material at the top end is melted, and the dry powder extinguishing agent sealed at the top end is released to extinguish fire. According to the invention, early warning, isolation and fire extinguishing are integrated, and the problem of thermal runaway of the lithium battery during parking can be adaptively solved.

The invention discloses an evaluation method of detection result accuracy of a rapid organophosphorus and carbamate pesticide residue detector. The evaluation method comprises the following steps of: (1) preparing a standard reference sample containing the effective components of organophosphorus and an carbamic acid ester; (2) determining an enzyme inhibition ratio reference value and standard deviation of the standard reference sample; (3) measuring the enzyme inhibition ratio of the standard reference sample by using the rapid organophosphorus and carbamate pesticide residue detector; (4) comparing an enzyme inhibition ratio detection value and the enzyme inhibition ratio reference value; and (5) obtaining an evaluation result. The evaluation method provided by the invention can effectively evaluate the detection result accuracy of the rapid organophosphorus and carbamate pesticide residue detector.

The invention discloses a detection kit and a detection method for pathogenic nucleic acid under an airtight condition, and belongs to the technical field of nucleic acid extraction. The detection kit comprises a sealing box body and a sealing cover, a first detergent chamber, a cracking buffering agent chamber, a second detergent chamber, an elution buffering agent chamber, a purification membrane chamber, a waste liquid chamber, a reaction detection chamber and a sealing membrane which is arranged on the channel and can be opened are sequentially arranged in the sealing box body from top to bottom, wherein the first detergent chamber, the cracking buffering agent chamber, the second detergent chamber, the elution buffering agent chamber, the purification membrane chamber, the waste liquid chamber and the reaction detection chamber are connected through the channel; and the detection method comprises the steps that the cracking buffering agent chamber, the first detergent chamber, the second detergent chamber and the elution buffering agent chamber are isolated from the channel through sealing films, after corresponding steps are completed, laser breaks through the sealing films, and liquid in corresponding cavities is centrifugally driven to flow into the channel. Preloaded reagents are isolated through the sealing films, laser drilling and centrifugal force driving are completely achieved in a non-contact mode, and on the basis of the chip structure design of fluid properties, pathogenic nucleic acid extraction and real-time fluorescent PCR amplification detection of the detection kit under the airtight and full-closed condition are achieved.

The invention relates to a method for determining VOCs in a packaging material through purging and trapping-gas chromatography-mass spectrometry. The method is characterized by comprising the steps ofsample preparation, purging and trapping and sample introduction, chromatography-mass spectrometry combined determination, qualitative confirmation, quantitative analysis and calculation result analysis. The method is scientific and reasonable in structural design, and the content of 25 kinds of volatile organic pollutants (VOCs) in the packaging material and products thereof can be determined bythe method for determining the VOCs in the packaging material through the purge-trap-gas chromatography-mass spectrometry; through the purging and trapping and sampling mode, extraction and concentration by using an organic solvent are avoided, so that the interference of an external solvent is reduced; compared with a headspace mode, the purging and trapping mode needs smaller area of the sample, and the sensitivity is higher; a gas chromatograph-mass spectrometer is used for detection, so that the qualitative ability of the method is enhanced, and the false positive risk is reduced; and patent test types are many, including alcohols, esters, ketones, benzenes, alkenes, halogenated hydrocarbons and the like, and the test requirements of VOCs in packaging materials at present are basically met.

The invention relates to a kit for detecting human ABCB1 gene polymorphism and application thereof. The materials in the box of the present invention include specific primers and specific fluorescent probes for detecting the SNP site rs1045642 on the ABCB1 gene, Taq DNA polymerase, dNTP mixture, MgCl 2 solution, fluorescent quantitative PCR reaction buffer and deionized water. The invention overcomes the respective defects of various gene mutation detection methods. The invention has the advantages of high detection accuracy, simple and convenient detection, short detection time, and simple result analysis, and is suitable for use in clinical laboratories. It can perform qualitative detection of CYP2C19 gene polymorphism sites, and perform PCR fluorescence amplification reactions according to SNP sites. , the results can be judged only according to whether the two different fluorescence curves are on the line, there will be no human error, the false positive and false negative rates are low, the detection cost is reduced, no DNA extraction is required, and the expansion can be completed only by lysing whole blood cells increase to improve detection accuracy.

The present invention discloses a detection kit for intelligence genes. The detection kit comprises a PCR reaction reagent, and specific primers and fluorescent probes for gene locus SNP detection, wherein the gene loci comprise JMJD1C gene locus rs7923609 and LRRC14 gene locus rs2721173. According to the present invention, the detection of different genotypes of the two loci can be completed in the single-tube PCR system through the detection kit of the present invention, such that the operation steps are less, the time consumption is short, the tube opening operation is not required, the possibility of the PCR product pollution is reduced, the specificity of the detection is high, and the result is easy to interpret.

The invention discloses a cable insulation online monitoring method based on KPCA-NSVDD. The cable insulation on-line monitoring method comprises the following steps: firstly, acquiring cable insulation historical data including a small number of fault samples as an original training data set; secondly, in order to solve the problem that high-dimensional data is difficult to process, using kernel principal component analysis (KPCA) for carrying out dimension reduction processing on the data; and then establishing an NSVDD (support vector data description with fault samples) training model by using the training sample, and reflecting the real-time state of cable insulation by using the absolute distance between the real-time sample and the constructed NSVDD hypersphere center. The sliding window mechanism is introduced, the training model can be updated in real time, and the anomaly recognition accuracy is improved. The established model can accurately grasp the behavior rule of normal work of the unit, and the operation state of the overhead line is monitored on line through the established model, so that timely early warning of abnormity is realized.

The invention relates to a kit for detecting human COX-2 gene polymorphism and an application thereof. The kit includes the following substances: a specific primer and a specific fluorescent probe for detecting an rs20417 SNP locus on COX-2 gene, a Taq DNA polymerase, a dNTP mixed liquid, a MgCl2 solution, a fluorescent quantitative PCR reaction buffer liquid and deionized water. The kit overcomes the defects in various gene mutation detection methods, is high in detection accuracy, is simple and convenient, is short in detection time and is simple in result analysis. The kit is suitable for clinical laboratories and can perform qualitative detection to COX-2 gene polymorphic sites, wherein a PCR fluorescent amplification reaction is carried out according to the SNP locus. A result can be determined just on the basis of whether two different fluorescent curves are positive or not, so that the kit is free of manual error, is low in false positive and false negative rate, and is improved in efficiency and reduced in detection cost.

The invention relates to an automobile urea quality sensor output signal control method, which comprises the following steps that a urea quality sensor is arranged in a urea box, and a certain amount of urea solution is filled in the urea box; a power supply of the urea quality sensor is switched on, and the urea quality sensor works normally and records voltage energy signals converted from five continuous ultrasonic echo signals; if the number of the energy signals higher than the threshold value line in the five signals recorded continuously is larger than or equal to 3, it is judged that the output signals of the sensor are reliable, and the ECU outputs the actually-calculated urea concentration value, and if the number of the energy signals higher than the threshold value line in the five signals recorded continuously is smaller than or equal to 2, it is judged that the output signals of the sensor are not reliable, and the sensor outputs invalid signal codes; and the electronic control unit ECU outputs the reliable signal which is output last time as a replacement signal. According to the method, the sensor signal judgment logic is optimized, so that the sensor output value is more reliable, and the system robustness and the customer driving experience are improved.

The invention belongs to the field of genetic gene detection, and in particular relates to a kit and a method for detecting the gene polymorphism of human MTHFR (methylene tetrahydrofolate reductase) based on a Taqman-MGB (Minor Groove Binder) probe. By adopting the kit and method, two SNP (single nucleotide polymorphism) sites namely C677t and A1298C can be detected at the same time, and the results are easy to judge and read. The MGB probe has a nonluminous 3'-end quenching group and a relatively low background, is more sensitive to detection of a single base mutation, and can judge and read different genotypes only according to a Ct value, and the result can be judged and read more easily compared with that of conventional methods of Taqman probe detection and HRM (high resolution melting) detection. The probe price is relatively low, the detection cost is relatively low, a polymorphism site only needs one-tube qPCR (quantitative polymerase chain reaction) detection, and the operation is simple. Moreover, the method disclosed by the invention does not need subsequent analysis of PCR products, so that while the detection cost is saved, the detection circle is greatly shortened, the detection efficiency is improved and the risk of false positive caused by PCR product pollution is reduced.

The invention is applicable to the technical field of biological detection, and provides a microfluidic chip for capturing and / or counting cells and its preparation method and application. The microfluidic chip includes: a first base layer; an antibody-modified inverse opal photonic crystal structure , set on the first base layer, which includes antibody modification layer and doped with Yb 3+ and Er 3+ The yttrium vanadate inverse opal structure layer; the second base layer is set on the side of the first base layer close to the antibody-modified inverse opal photonic crystal structure; the microfluidic cavity is set between the first base layer and the second base layer. The invention controls the photonic bandgap by regulating the minimum repeating unit size of the photonic crystal, and makes double-layered Yb-doped materials with different apertures and different bandgaps. 3+ and Er 3+ The inverse opal structure of yttrium vanadate can significantly enhance the upconversion green light emission through the bandgap effect of photonic crystals, which solves the problem of insufficient fluorescence intensity caused by the low quantum efficiency of the existing upconversion luminescence process.

The invention belongs to the gene detecting technology in clinical detecting technologies of the bio-medical field and particularly relates to a kit for quickly detecting polymorphism of a human CYP2C19 gene and a using method of the kit. The kit comprises three pairs of primers, three probes, six PNA (pentose nucleic acid) sequences, a pair of beta-Actin interior label primers and an interior label probe, and further comprises a PCR (polymerase chain reaction) buffer solution of Mg<2+>, a dNTP mixture, Taq enzyme and ddH2O. The kit can be utilized to carry out qualitative detection on a polymorphic site of the CYP2C19 gene and carry out two-tube PNA-PCR fluorescent amplified reaction according to the SNP site and can carry out result judgment according to a condition whether a two-tube fluorescence curve is started or not without producing personal errors, so that a false positive rate and a false negative rate are low. And moreover, the kit can be used to realize high-flux detection easily and is capable of clinical use.

The invention discloses an acrylonitrile-styrene copolymer material for promoting ultrasonic attenuation and a preparation method thereof, belonging to the technical field of polymer materials. The acrylonitrile-styrene copolymer material for promoting ultrasonic attenuation according to the present invention comprises the following components in parts by weight: 40-90 parts of acrylonitrile-styrene resin and 10-50 parts of ultrasonic attenuation agent; the ultrasonic attenuation agent comprises acrylonitrile -Styrene-Acrylate Copolymer, Acrylonitrile-Styrene-Butadiene Copolymer, Acrylonitrile-Styrene-Ethylene-propylene Rubber Copolymer, Acrylate-Styrene-Butadiene Copolymer, Styrene-Ethylene- At least one of butene-styrene copolymers. The acrylonitrile-styrene copolymer material prepared by the formula of the present invention can make the radar wave signal of false alarm to be lost in the transmission process, and can be used to prepare the grille, bumper and car logo of the vehicle equipped with the ultrasonic radar wave detector and other auto parts.