Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

SNP molecular marker related to lambing number of sheep single birth, primer probe set, kit, detection method and application

A technology of molecular markers and primer probes, which is applied in the field of sheep SNP molecular markers and SNP molecular markers, can solve the problem of no SNP molecular markers found in singleton lambs, achieve easy interpretation of results, save testing costs, and shorten testing cycles Effect

Inactive Publication Date: 2019-04-19
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, no SNP molecular markers closely related to the number of singleton lambs in sheep have been found in the LEPR gene

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • SNP molecular marker related to lambing number of sheep single birth, primer probe set, kit, detection method and application
  • SNP molecular marker related to lambing number of sheep single birth, primer probe set, kit, detection method and application
  • SNP molecular marker related to lambing number of sheep single birth, primer probe set, kit, detection method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1. Experimental materials

[0053] 342 healthy ewes of Small Tail Han sheep were selected as the test objects.

[0054] 2. Genomic DNA extraction

[0055] 1ml of blood was collected from the jugular vein of the sheep and treated with EDTA anticoagulant. First, the red blood cell lysate is lysed to remove DNA-free red blood cells. The nucleus lysate lyses the cells to release genomic DNA. Then the protein precipitation solution selectively precipitates to remove the protein. Finally, the purified genomic DNA is precipitated by isopropanol and redissolved in the DNA lysate.

[0056] 3. Genotyping by TaqmanMGB probe method

[0057] Primers and probes were designed for the 40857869bp site on sheep chromosome 1 (NM_001009763.1, based on the sheep genome sequence information version number Oar_v3.1).

[0058] The nucleotide sequence of the primer is as follows:

[0059] Forward primer: 5'-AGCCGAGGAGGAGCAAGG-3';

[0060] Reverse primer: 5'-TCCCATGATCTCTTAGAGGAAGACTC-3'. ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an SNP molecular marker related to lambing number of a sheep single birth, a primer probe set, a kit, a detection method and application, and belongs to the technical field of sheep SNP molecular markers. The SNP molecular marker is located on a No.40857869bp site on a No.1 chromosome of sheep, the site is located in an LEPR gene, C / T base mutation exists in the site, the gene type of the LEPR gene is CC or CT or TT, the lambing number of the sheep single birth of the gene type CC is lager than that of the gene type TT, and the lambing number of the sheep single birth ofthe gene type TT is larger than that of the gene type CT; a sheep gene group sequence information which SNP molecular marker information is based on is Oar_v3.1. The SNP molecular marker is related to the lambing number of the sheep single birth remarkably, and according to the SNP molecular marker, the lambing capacity of the sheep single birth can be judged. In practical application, ewes in aCC type shall be selected as reserved ewes, and the heterozygous ewes and the ewes in a CT type are removed during selection.

Description

technical field [0001] The invention relates to the technical field of sheep SNP molecular markers, in particular to the SNP molecular markers, primer probe sets, kits, detection methods and applications related to sheep singleton litter size. Background technique [0002] In my country, except for a small number of sheep breeds such as small-tailed Han sheep and Hu sheep, which produce multiple lambs, most of the other sheep breeds produce single lambs, which greatly restricts the development of my country's sheep industry. Lamb size is a complex trait controlled by multiple genes with low heritability, and it is difficult to obtain large genetic progress through traditional phenotypic selection. Therefore, it is very important to study the genes related to sheep litter size to improve sheep reproductive efficiency, speed up sheep breeding process, and improve the economic benefits of sheep industry. With the deepening of the study on lambing traits, many main genes that c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 储明星王翔宇于嘉瑞郭晓飞狄冉刘秋月胡文萍
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com