Microfluidic chip for capturing and/or counting cells, its preparation method and application

A microfluidic chip and microfluidic cavity technology, applied in chemical instruments and methods, biochemical equipment and methods, enzymology/microbiology devices, etc., to achieve the effects of good biocompatibility, low price, and easy production

Active Publication Date: 2022-04-01
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, the capture efficiency of circulating tumor cells by existing methods still needs to be improved, and the real-time monitoring of the capture process still needs to be improved; in addition, because the number of circulating tumor cells in the blood is very small and there are many types of cells in the blood, it is difficult for the monitored cells to The visualization of the capture chip, monitoring, resolution, and counting at any time put forward higher requirements

Method used

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  • Microfluidic chip for capturing and/or counting cells, its preparation method and application
  • Microfluidic chip for capturing and/or counting cells, its preparation method and application
  • Microfluidic chip for capturing and/or counting cells, its preparation method and application

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Embodiment 1

[0059] This embodiment provides a method for preparing a microfluidic chip, which includes the following steps:

[0060] S1. Preparation of polymethyl methacrylate (PMMA) solution:

[0061] (1) Prepare 15mg / mL NaOH solution for later use. Pour an appropriate amount of methyl methacrylate (MMA) into the cup, add the prepared NaOH solution, shake for 1.5min, the NaOH solution will wash away the polymerization inhibitor in the MMA, let it stand for stratification, and use a syringe to drain the lower layer of waste liquid Extract and discard. Repeat more than 4 times, do not take out the last time.

[0062] (2) Put a rotor of appropriate size into the three-necked flask, then put the above-mentioned cleaned MMA 6mL, water 80mL, and potassium persulfate 38mg into the three-necked flask, connect the condenser tube, and block the non-condensing port of the three-necked flask. Put all the above into an oil bath mixer, make the liquid level in the three-necked bottle lower than the...

Embodiment 2

[0078] This embodiment provides a method for preparing a microfluidic chip, which includes the following steps:

[0079] S1. Preparation of polymethyl methacrylate (PMMA) solution:

[0080] (1) Prepare 15mg / mL NaOH solution for later use. Pour an appropriate amount of methyl methacrylate (MMA) into the cup, add the prepared NaOH solution, shake for 1 min, the NaOH solution will wash away the polymerization inhibitor in the MMA, let it stand for stratification, and use a syringe to pump out the waste liquid in the lower layer mention discarded. Repeat more than 4 times, do not take out the last time.

[0081] (2) Put a rotor of appropriate size into the three-necked flask, then put the above-mentioned cleaned MMA 6mL, water 80mL, and potassium persulfate 35mg into the three-necked flask, connect the condenser tube, and block the non-condensing port of the three-necked flask. Put all the above into an oil bath mixer, make the liquid level in the three-necked bottle lower than...

Embodiment 3

[0091] This embodiment provides a method for preparing a microfluidic chip, which includes the following steps:

[0092] S1. Preparation of polymethyl methacrylate (PMMA) solution:

[0093] (1) Prepare 15mg / mL NaOH solution for later use. Pour an appropriate amount of methyl methacrylate (MMA) into the cup, add the prepared NaOH solution, shake for 2 minutes, the NaOH solution will wash away the polymerization inhibitor in the MMA, let it stand for stratification, and use a syringe to pump out the waste liquid in the lower layer mention discarded. Repeat more than 4 times, do not take out the last time.

[0094] (2) Put a rotor of appropriate size into the three-necked flask, then put the above-mentioned cleaned MMA 6mL, water 80mL, and potassium persulfate 40mg into the three-necked flask, connect the condenser tube, and block the non-condensing port of the three-necked flask. Put all the above into an oil bath mixer, make the liquid level in the three-neck bottle lower th...

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Abstract

The invention is applicable to the technical field of biological detection, and provides a microfluidic chip for capturing and / or counting cells and its preparation method and application. The microfluidic chip includes: a first base layer; an antibody-modified inverse opal photonic crystal structure , set on the first base layer, which includes antibody modification layer and doped with Yb 3+ and Er 3+ The yttrium vanadate inverse opal structure layer; the second base layer is set on the side of the first base layer close to the antibody-modified inverse opal photonic crystal structure; the microfluidic cavity is set between the first base layer and the second base layer. The invention controls the photonic bandgap by regulating the minimum repeating unit size of the photonic crystal, and makes double-layered Yb-doped materials with different apertures and different bandgaps. 3+ and Er 3+ The inverse opal structure of yttrium vanadate can significantly enhance the upconversion green light emission through the bandgap effect of photonic crystals, which solves the problem of insufficient fluorescence intensity caused by the low quantum efficiency of the existing upconversion luminescence process.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a microfluidic chip for capturing and / or counting cells, a preparation method and application thereof. Background technique [0002] Although cancer patients are difficult to diagnose in the early stage and the disease develops rapidly, 90% of early cancers can be cured, and the key lies in early diagnosis and early treatment. Microfluidic technology has become a popular technology for early detection of cancer. Compared with traditional magnetic enrichment, size screening, gradient centrifugation and other methods, microfluidic technology has the advantages of specific selection, less damage to cells and real-time monitoring. [0003] Circulating tumor cells are cells that flow from the primary tumor into the vasculature or lymphatic vessels and travel with the body's circulatory system. Therefore, they can grow additional tumors in other important tis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/34B01L3/00
CPCC12M23/16C12M41/36B01L3/502707B01L3/502715B01L3/502761B01L2200/0668B01L2300/02B01L2300/0887B01L2300/12B01L2300/021
Inventor 董彪吕杰凯李春霞孙娇宋宏伟王林白雪徐琳
Owner JILIN UNIV
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