Proteome biomarkers of milk of different processing technologies and screening method and application thereof
A technology of biomarkers and processing technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to distinguish pasteurized milk and ESL milk, and can not be distinguished, so as to achieve rapid automatic identification, convenient screening method, The effect of improving detection efficiency
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[0097] 1) Sample preparation:
[0098] The milk sample was degreased by centrifugation (12000g, 4°C, 30min centrifugation), and the upper fat layer was removed. Draw 2mL of the subnatant and acidify it with 20% (v / v) acetic acid solution to pH 4.6, and centrifuge (10000g, 4°C, 30min) to precipitate casein. The solid-phase extraction column was pretreated with 3mL methanol and 3mL ultrapure water in advance, and the above supernatant was loaded onto a Waters SPE column (Oasis HLB, 60mg / 3mL), rinsed with 3mL 10% methanol water (v / v), blown Dry. Peptides were eluted from the column using 3 mL of pure methanol. The elution was collected and blown to dryness at 40°C with nitrogen. The obtained residue was redissolved to 1 mL with 0.1% formic acid water (v / v), and shaken for 5 min. Pass through a 0.22 μm syringe filter to the injection vial for detection on the machine.
[0099] 2) Data collection and preprocessing: Use ultra-high performance liquid chromatography in series wit...
Embodiment 1
[0102] Collection and preparation of milk samples
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