A method for rapid selection of three-line rice maintainer lines and sterile lines using rice genome analysis technology

A technology for genome analysis and maintenance, applied in the fields of botany equipment and methods, biochemical equipment and methods, plant genetic improvement, etc. Shorten the breeding period and solve the effect of the long period

Active Publication Date: 2022-08-09
RICE RES INST GUANGDONG ACADEMY OF AGRI SCI
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Problems solved by technology

[0005] In order to overcome the above-mentioned deficiencies in the prior art, the present invention proposes a method for rapidly selecting and breeding three-line rice maintainer lines and sterile lines using the rice genome analysis technology, which solves the problems of the existing three-line rice male-sterile line breeding methods that have a long lifespan, Problems of narrow genetic base and difficult aggregation of good traits

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  • A method for rapid selection of three-line rice maintainer lines and sterile lines using rice genome analysis technology
  • A method for rapid selection of three-line rice maintainer lines and sterile lines using rice genome analysis technology
  • A method for rapid selection of three-line rice maintainer lines and sterile lines using rice genome analysis technology

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Embodiment 1

[0032]Example 1 A method for rapid selection of three-line rice maintainer lines and sterile lines using rice genome analysis technology

[0033] Guang 8A is an excellent sterile line (the corresponding maintainer line is Guang 8B) bred by our unit (Rice Research Institute of Guangdong Academy of Agricultural Sciences). The sterile line has the quality of Zengcheng silk seedling rice, strong combining ability and comprehensive agronomic traits excellent. In July 2010, it passed the technical appraisal of Guangdong Province. At present, there are 34 hybrid combinations that have passed the provincial approval. Three-line hybrid rice combinations such as Guang 8 You 169, Guang 8 You 2168, Guang 8 You Jinzhan and Guang 8 You 165, which were bred with the Guang 8A pairing group, have been selected as the leading varieties in Guangdong Province for many times, and have a large area in the province. Promote planting. The major defect of the Guangzhou 8A sterile line is the suscept...

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Abstract

The invention belongs to the technical field of rice breeding, and in particular relates to a method for rapidly selecting a three-line rice maintainer line and a sterile line by utilizing the rice genome analysis technology. Second, the sterile plants of the progeny in the same combination were used as the control to compare the fertile progeny maintainers in the same combination through genome-wide comparison, and the maintainers carrying the minor restorer genes were quickly eliminated, so that conventional rice with favorable traits could be replaced. , restorer lines or landraces are used as donor males to solve the problem of the narrow genetic basis of the existing maintainer lines; in addition, large population marker screening is used to break the linkage burden of bad alleles, and then the genotypes are marked by whole-genome SNP markers. The cluster analysis of the hybrid rice is carried out by selecting sterile plants and maintainer plants with similar genetic backgrounds for pairwise hybridization, so as to solve the problem of difficult aggregation of excellent traits, and has important application value in the breeding of hybrid rice.

Description

technical field [0001] The invention belongs to the technical field of rice breeding, in particular to a method for rapidly selecting a three-line rice maintainer line and a sterile line by utilizing the rice genome analysis technology. Background technique [0002] Hybrid rice breeding technology is an important technology to ensure my country's food security. Over the years, Chinese three-line hybrid rice has been widely used in China and the world because of its high and stable yield and relatively safe seed production. However, due to the limitation of the genetic characteristics of the sterility of the three-line rice sterile line, the selection of the three-line sterile line requires the improvement of the maintainer line. The cultivation methods of maintainer lines are mostly realized by crossing between maintainer lines, which limits the application of new excellent genes and new germplasm resources in three-line maintainer lines and sterile lines, and restricts the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895A01H1/02A01H1/04
CPCA01H1/02A01H1/023A01H1/04A01H1/045C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王重荣周少川黄道强周德贵王志东
Owner RICE RES INST GUANGDONG ACADEMY OF AGRI SCI
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