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Anti-pollution nucleic acid amplification substance rapid detection device and method and application

A detection device and anti-pollution technology, which is applied in biochemical cleaning devices, biochemical equipment and methods, enzymology/microbiology devices, etc., can solve the problems of inconsistency in complexity and multiple virus nucleic acid extraction schemes, and achieve simple use , reduce the risk of cross-infection, the effect of convenient operation

Pending Publication Date: 2022-02-18
ANHUI WEIZHEN BIOLOGICAL ENG TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Nucleic acid extraction has become the most important and basic operation in molecular biology experimental techniques, but at present there are many extraction schemes for viral nucleic acid, and they are complicated and simple.

Method used

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  • Anti-pollution nucleic acid amplification substance rapid detection device and method and application
  • Anti-pollution nucleic acid amplification substance rapid detection device and method and application
  • Anti-pollution nucleic acid amplification substance rapid detection device and method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Embodiments of the present invention provide a rapid detection device for anti-pollution nucleic acid amplification products, such as figure 1 As shown, it includes a detection inner core 1 and a sealed outer box 2,

[0040] The sealed outer box 2 includes a sealed cover 21, an upper casing 22 and a lower casing 23 arranged in sequence;

[0041] The upper case 22 includes an upper end 221 and a lower end 222, the lower end 222 is fastened to the lower case 23 to form a first accommodation space 223, and the detection inner core 1 is disposed in the first accommodation space 223;

[0042] Such as figure 2 and image 3 As shown, the sealing cover 21 is fastened to the upper end 221 of the upper casing 22 to form a second accommodation space 224. A fixing bracket 225 can be arranged in the second accommodation space 224, and two PCR reaction tubes are clamped on the fixing bracket 225. component 3;

[0043] In the embodiment of the present invention, the second accomm...

Embodiment 2

[0057] The embodiment of the present invention discloses a rapid detection method for anti-pollution nucleic acid amplification product, comprising the following steps:

[0058] a) Take off the sealing cover;

[0059] b) Put the warmed two reaction tubes into the second accommodation space of the anti-pollution nucleic acid amplification product rapid detection device described in Example 1 and fix them on the fixed support through the needle plate; specifically, the amplified The unopened PCR reaction tube is placed in the PCR tube hole of the fixed bracket, and the PCR reaction tube includes a reaction solution tube and a buffer tube in the embodiment of the present invention;

[0060] c) Press down the two reaction tubes, and the needle disk punctures the bottom of the reaction tube; d) Manually squeeze the side wall of the reaction tube, the reaction liquid passes through the needle disk under the action of gravity and pressure and is absorbed and diverted by the water-con...

Embodiment 3

[0067] The present invention also discloses the above-mentioned rapid detection device for anti-pollution nucleic acid amplification products or the above-mentioned rapid detection method for anti-pollution nucleic acid amplification products in the food industry, agriculture, animal husbandry, customs quarantine, gene mutation detection and DNA single nucleotide polymorphism applications in sex identification.

[0068] It should be noted that, as a general technical platform for the detection of closed nucleic acid amplification products, the method of the present invention and its device can be widely used in the clinical detection of infectious disease pathogens, strategic technical reserves for public health emergencies, agriculture and animal husbandry Industry and food industry, customs inspection and quarantine, environmental testing, biological weapons testing, human genetic disease testing, premarital and prenatal inspections, prenatal and postnatal care, forensic iden...

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Abstract

The invention discloses an anti-pollution nucleic acid amplification substance rapid detection device, which comprises a detection inner core and a sealing outer box, and a water guide fiber membrane is arranged at a corresponding position of the lower end of a needle disc; a tube body is used for accommodating a reaction solution and a hybridization solution, and when the needle disc punctures the inverted top of the tube body and the side wall of the tube body is manually extruded or the rapid detection device is shaken by manual pressing, the reaction solution passes through the needle disc under the action of gravity and pressure or centrifugal force and is adsorbed and guided by the water guide fiber membrane to enter a test strip; the invention also correspondingly discloses a use method of the anti-pollution nucleic acid amplification substance rapid detection device. According to the invention, liquid in the reaction tube flows out to a specified detection card in a completely closed environment, and the device has the advantages of good sealing effect and prevention of primer aerosol pollution.

Description

technical field [0001] The invention relates to the field of nucleic acid detection, in particular to a rapid detection device, method and application of anti-pollution nucleic acid amplification products. Background technique [0002] 2019-nCoV belongs to the genus β-coronavirus, with an envelope, and the particles are round or oval, often pleomorphic, with a diameter of 60-140nm. It is significantly different from SARSr-CoV and MERSr-CoV based on characteristics. Current research shows that it has more than 85% homology with bat SARS-like coronavirus (bat-SL-CoVZC45). Based on the current epidemiological investigation, the incubation period is 1-14 days, mostly 3-7 days. Most of the clinical manifestations are mild, with fever, dry cough, and fatigue as the main manifestations, but there are also severe patients with dyspnea and / or hypoxemia after a week, and severe cases can quickly progress to acute respiratory distress syndrome, septic shock, Refractory metabolic aci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12M1/24C12Q1/6804
CPCC12Q1/6804C12Q2531/113C12Q2563/131C12Q2565/625
Inventor 孙克茂高鹏渠香云赵宝库
Owner ANHUI WEIZHEN BIOLOGICAL ENG TECH CO LTD
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