PCR amplification device

Abstract

The invention provides a PCR (polymerase chain reaction) amplification device which comprises a heating module. The heating module comprises a fixed base, a fixed sleeve is arranged above the fixed base, and an open cavity is formed in the fixed sleeve. Two oppositely arranged side vertical walls of the open cavity can be respectively attached to the walls of a reaction container of a micro-droplet chip accommodated in the open cavity, and heating sheets are respectively arranged on the outer side surfaces, far away from the fixed sleeve, of the two side vertical walls. According to the invention, the two oppositely arranged heating sheets can heat the two opposite walls of the reaction container of the micro-droplet chip in the fixed sleeve at the same time, and compared with the bottom heating mode of the reaction container in the prior art, the heating contact area is increased, and the PCR amplification efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of digital PCR analyzers, and in particular relates to a PCR amplification device. Background technique [0002] Digital PCR is the latest quantitative technology. Nucleic acid quantification based on single-molecule PCR method is an absolute quantitative method. It mainly adopts the microfluidic or microdroplet method in the current hot research field of analytical chemistry to dilute a large amount of The final nucleic acid solution is dispersed into the microreactor or microdroplet of the biochip, and the number of nucleic acid templates in each reactor is less than or equal to 1, and after the PCR module amplification cycle, there is a reaction of 1 nucleic acid molecule template A reactor will give a fluorescent signal, and a reactor without a template will have no fluorescent signal. According to the relative ratio and the volume of the reactor, the nucleic acid concentration of the original solution ...

AI Technical Summary

Problems solved by technology

[0004] The PCR module is an essential link in the process of nucleic acid quantitative detection. The high efficiency and stability of the PCR module and the amplification time directly affect the time of nucleic acid detection. Most of the PCR modules currently on the market are heated by heating plates on the bottom of the sample. The heating area is relatively small, so it is difficult to increase the heating and cooling speed, and the amplification time is about 2 hours, which limits the speed of nucleic acid detection

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