A cartilage tissue engineering scaffold and its preparation method and application

Abstract

The invention discloses a cartilage tissue engineering scaffold, a preparation method and application thereof. It includes the following steps of preparation: loading growth factors on nanoparticles to obtain growth factor-loaded nanoparticles; then surface-treating them to make their surfaces have reactive active groups; Diol aqueous solution is mixed with the following 1) and / or 2) to obtain hydrogels loaded with growth factors A and B; 1) nanoparticles loaded with growth factor A and nanoparticles loaded with growth factor B; 2) growth factor A loaded and growth factor B nanoparticles; growth factor C is loaded on the hydrogel loaded with growth factors A and B to obtain a cartilage tissue engineering scaffold; wherein, growth factor A, growth factor B and growth factor C all promote cartilage regeneration different growth factors. The scaffold of the invention simulates the structure and function of natural cartilage tissue, and carries multiple growth factors to express synergistically and act on cartilage repair and regeneration.

Description

A kind of cartilage tissue engineering scaffold and preparation method and application thereof technical field The present invention relates to a kind of cartilage tissue engineering scaffold and preparation method and application thereof, belong to tissue engineering scaffold preparation field. Background technique For cartilage tissue engineering, biodegradable scaffolds serve to support and induce cell growth and reproduction, regulation Determine the shape and size of regenerative tissue, and ultimately combine with body tissue, together with cells and growth factors Three elements of tissue engineering. At present, although there are many reports on cartilage tissue engineering using scaffolds with different materials, There is still a gap between the structure and function of cartilage tissue obtained from these scaffolds compared with innate cartilage tissue. Therefore, to form Tissue engineered cartilage with similar structure and function to innate carti...

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Problems solved by technology

[0003] PEG hydrogels have been widely used in biomedical applications due to their non-immunogenicity, excellent hydrophilic properties, and good biocompatibility, but their small size and three-dimensional network structure pores make it difficult for cells to enter and grow. The anti-adhesion property of protein makes it difficult for cells to adhere and grow on it, and the good hydrophilicity makes it release the growth factors loaded quickly, so PEG hydrogel is not ideal as a tissue engineering scaffold

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