Hydroxyapatite/saccharomycetes biological hybrid material as well as preparation method and application thereof

A hydroxyapatite, bio-hybrid technology, applied in plant raw materials, active ingredients of phosphorus compounds, plant/algae/fungus/moss ingredients, etc., can solve the problems of poor tumor treatment effect and difficult tumor inhibition, and achieve dispersive Good stability, low price, and enhanced tumor calcium overload killing effect

Active Publication Date: 2022-03-11
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the embodiment of this application is to provide a hydroxyapatite / yeast biohybrid material and its preparation method and application, so as to solve the problem of single yeast tumor treatment The effect is poor, and it is difficult to suppress the tumor strongly

Method used

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  • Hydroxyapatite/saccharomycetes biological hybrid material as well as preparation method and application thereof
  • Hydroxyapatite/saccharomycetes biological hybrid material as well as preparation method and application thereof
  • Hydroxyapatite/saccharomycetes biological hybrid material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] This example provides the preparation of a kind of HA / Yeast biohybrid material, comprises:

[0044] Step (1), prepare Ca(NO 3 ) 2 solution and ammonium phosphate solution and adjust the pH separately for use;

[0045] Specifically, a certain Ca(NO 3 ) 2 Dissolved in deionized water to obtain 17mM Ca(NO 3 ) 2 solution, and then adjust the pH to 8 with 1M NaOH solution. A certain amount of ammonium phosphate was dissolved in deionized water to obtain a 10 mM ammonium phosphate solution, and then the pH was also adjusted to 8 with 1 M NaOH.

[0046] Step (2), dispersing the yeast in deionized water after shaking overnight in the liquid medium to obtain a yeast dispersion for use;

[0047] Specifically, put a certain amount of yeast into the medium and shake overnight at 36°C, then centrifuge the yeast to remove the medium, wash it twice with deionized water, then disperse the yeast in deionized water, and configure the concentration 0.8×10 8 CFU / mL of yeast soluti...

Embodiment 2

[0051] This example provides the preparation of a kind of HA / Yeast biohybrid material, comprises:

[0052] Step (1), prepare Ca(NO 3 ) 2 solution and ammonium phosphate solution and adjust the pH separately for use;

[0053] Specifically, a certain Ca(NO 3 ) 2 Dissolved in deionized water to obtain 19mM Ca(NO 3 ) 2 solution, and then adjust the pH to 10 with 1M NaOH solution. A certain amount of ammonium phosphate was dissolved in deionized water to obtain a 12 mM ammonium phosphate solution, and then the pH was also adjusted to 10 with 1 M NaOH.

[0054] Step (2), dispersing the yeast in deionized water after shaking overnight in the liquid medium to obtain a yeast dispersion for use;

[0055] Specifically, put a certain amount of yeast into the medium and shake overnight at 38°C, then centrifuge the yeast to remove the medium, wash it twice with deionized water, then disperse the yeast in deionized water, and configure the concentration 1.2×10 8 CFU / mL of yeast solu...

Embodiment 3

[0059] Preferably, this example provides a kind of preparation of HA / Yeast biohybrid material, comprising:

[0060] Step (1), prepare Ca(NO 3 ) 2 solution and ammonium phosphate solution and adjust the pH separately for use;

[0061] Specifically, a certain Ca(NO 3 ) 2 Dissolved in deionized water to obtain 18mM Ca(NO 3 ) 2 solution, and then adjust the pH to 9 with 1M NaOH solution. A certain amount of ammonium phosphate was dissolved in deionized water to obtain a 10.8 mM ammonium phosphate solution, and then the pH was also adjusted to 9 with 1 M NaOH.

[0062] Step (2), dispersing the yeast in deionized water after shaking overnight in the liquid medium to obtain a yeast dispersion for use;

[0063] Specifically, put a certain amount of yeast into the medium and shake overnight at 37°C, then centrifuge the yeast to remove the medium, wash it twice with deionized water, then disperse the yeast in deionized water, and configure the concentration 1×10 8 CFU / mL of yea...

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Abstract

The invention discloses a hydroxyapatite/saccharomycetes biological hybrid material as well as a preparation method and application thereof, and relates to the field of tumor treatment, and the preparation method comprises the following steps: vibrating saccharomycetes in a liquid culture medium overnight, and dispersing in deionized water to obtain a saccharomycetes dispersion liquid; and respectively taking a Ca (NO3) 2 solution and an ammonium phosphate solution with the same volume, mixing, adding the yeast dispersion liquid, stirring, and centrifugally cleaning to obtain the HA/Yeast biological hybrid material. On the basis of the HA/Yeast biological hybrid material, tumor calcium overload and tumor immune collaborative treatment can be realized, and the problem that the tumor killing effect of a single way is relatively weak is solved. The HA/Yeast nano-particles disclosed by the invention are simple in synthesis method, good in dispersity and uniform in size; the yeast can achieve tumor specific targeting by tending to an acid environment, H2S is generated through fermentation to enhance tumor calcium overload, meanwhile, tumor immunotherapy is activated, and finally tumor synergistic killing is achieved.

Description

technical field [0001] The present application relates to the field of biohybrid materials, in particular to a hydroxyapatite / yeast biohybrid material and its preparation method and application. Background technique [0002] In the past ten years, with the development of industrialization, the improvement of living standards and the increase of average life expectancy, cancer, as a very difficult disease, has seriously threatened human health. The situation in our country is still very serious. According to data from the Cancer Center, cancer has become the number one cause of death, accounting for 23% of all deaths among residents. Due to the diversity, complexity and heterogeneity of cancer, the morbidity and mortality of cancer diseases are still increasing rapidly worldwide despite the variety of cancer treatment methods. [0003] Traditional cancer treatment methods have their own limitations. At present, the methods used clinically for cancer treatment mainly include...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/42A61K33/04A61K36/064A61K31/716A61K47/46A61P35/00A61P37/04
CPCA61K36/064A61K33/42A61K33/04A61K31/716A61K47/46A61P35/00A61P37/04A61K2300/00
Inventor 李翔胡泽峰方超傅译可
Owner ZHEJIANG UNIV
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