Biological reaction device

Abstract

The invention discloses a biological reaction device which comprises a flow guide container, a pressure applying mechanism and a carrier column which are arranged in a reaction cavity, the carrier column comprises a first barrel and a second barrel which are sleeved with each other, a first window is formed in the side wall of the first barrel, a second window is formed in the side wall of the second barrel, and a closed cavity is formed between the first barrel and the second barrel. A folding carrier with a plurality of folding surfaces is arranged in the cavity; the pressure applying mechanism is used for pressing culture liquid which flows into the second chamber of the reaction chamber from the first chamber of the reaction chamber through the flow guide container into the first cylinder, so that the culture liquid flows unidirectionally through the first window and is filled into the cavity, and then further flows unidirectionally through the second window and is filled into the first chamber; and finally, the water flows into the second cavity through the flow guide container and is pressed and fed into the first barrel to form circulation. The whole carrier can be taken out, cell death caused when cell culture fluid is extracted can be prevented, the surface area of the carrier is remarkably increased, and large-scale culture of various animal cells can be achieved.

Description

technical field [0001] The invention relates to the technical field of biopharmaceutical equipment, in particular to a biological reaction device. Background technique [0002] Bioreactor is an important equipment in the pharmaceutical industry, and its function is to cultivate cells for medical experiments. [0003] Currently, commonly used bioreactors include fluidized bed reactors and fixed bed reactors. However, the current bioreactors, whether they are fluidized bed reactors or fixed bed reactors, have the following problems: [0004] First of all, it is necessary to fill the bioreactor with granular cell culture carriers, which is not only time-consuming and laborious, but also difficult to operate. Moreover, due to the influence of cell metabolism and the distribution of cell culture medium, it is very difficult to expand the capacity of the bioreactor by using granular carriers for cell culture. [0005] At the same time, after the cell culture is completed, the g...

AI Technical Summary

Problems solved by technology

[0004] First of all, it is necessary to fill the bioreactor with granular cell culture carriers, which is not only time-consuming and laborious, but also difficult to operate

Moreover, due to the influence of cell metabolism and the distribution of cell culture medium, it is very difficult to expand the capacity of the bioreactor by using granular carriers for cell culture.

[0005] At the same time, after the cell culture is completed, the granular cell culture carrier must be fished out by manpower and tools such as net bags, which is difficult to handle repeatedly, which greatly affects the efficiency of the bioreactor.

[0006] Especially for the fluidized bed reactor, when the cell culture medium is extracted, the cell culture carrier will also be extracted, which will cause greater difficulties in operation

Images