LAMP (loop-mediated isothermal amplification) primer group and detection method for rapidly detecting strawberry aphelenchus destructor

Abstract

The invention discloses an LAMP (loop-mediated isothermal amplification) primer group and a detection method for rapidly detecting strawberry aphelenchoides, and belongs to the field of molecular biological detection of plant diseases. The LAMP primer group comprises a pair of outer primers, a pair of inner primers and a loop primer, wherein the nucleotide sequences of the primers are respectively as shown in SEQ ID NO.1-SEQ ID NO.5. The method comprises the following steps: by taking DNA of a sample to be detected as a template, carrying out LAMP amplification by utilizing an LAMP primer group; and judging whether the to-be-detected sample contains the strawberry aphelenchus destructor or not according to an amplification result. The method is rapid and convenient; the specificity is high; the sensitivity is high; the steps are simple; result determination is simple; meanwhile, the method is safe to human and environment, toxic reagents such as EB do not need to be used in the detection process, and the detection requirement for rapidly and accurately identifying strawberry aphelenchus destructor in a first-line laboratory of inspection and quarantine can be met.

Description

technical field [0001] The invention belongs to the field of molecular biology detection of plant diseases, and in particular relates to a LAMP primer set and a detection method for rapidly detecting the nematode sciatica. Background technique [0002] Strawberry fragariae (Aphelenchoides fragariae) belongs to the nematode phylum, Aphelenchoides, Aphelenchoides, Aphelenchoides suborder, Aphelenchoides, Aphelenchoides subfamily, Aphelenchoides genus. It is a leaf and bud parasitic nematode that can infect a variety of crops and ornamental plants. It is a quarantine pest in my country. [0003] Strawberry slippery nematode can harm strawberry, an important economic crop, and does not directly invade the plant tissue. It feeds on the growth point of strawberry by ectoparasites, causing the leaves to shrink and twist, and the leaves are often small; the feeding activities of the nematode can cause strawberry fruit Buds are reduced, resulting in reduced strawberry yield, causing...

AI Technical Summary

Problems solved by technology

However, the conventional specific primer PCR method has high requirements for equipment: PCR instrument, gel electrophoresis instrument and other equipment are required

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