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Protein purification device and method based on electromembrane filtration technology

A technology of protein purification and filtration technology, applied in the field of protein purification devices based on electric membrane filtration technology, can solve the problems of reduced membrane permeation flux, high degree of membrane fouling, clogging of membrane pores, etc., to improve membrane filtration effect and reduce membrane Pollution degree and the effect of prolonging the service life of the membrane

Pending Publication Date: 2022-04-12
JILIN UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, the degree of membrane fouling in the process of membrane filtration and purification is relatively high, which greatly reduces the permeation flux of the membrane, blocks the membrane pores, and reduces the purification effect.

Method used

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  • Protein purification device and method based on electromembrane filtration technology
  • Protein purification device and method based on electromembrane filtration technology

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Embodiment Construction

[0027] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention.

[0028] Such as figure 1 As shown, a device for selectively purifying proteins in apple peels based on electromembrane filtration technology, including a liquid inlet system 1, a power generation system 2, a filtration system 3 and a liquid outlet system 4.

[0029] Such as figure 1 As shown, as a preferred embodiment of the present invention, the liquid inlet system includes a vacuum pump 1-1 and a liquid storage bottle 1-2, and the liquid storage bottle 1-2 is sealed with a soft rubber stopper. An inlet pipe and a liquid outlet pipe are provided, and one end of the air outlet pipe is connected to the vacuum pump 1-1 through a conduit, and one end of the liquid outl...

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Abstract

The invention relates to the technical field of protein purification, and discloses a protein purification device based on an electric membrane filtration technology and a method thereof in order to solve the problem that the membrane pollution degree is high in the filtration process when an existing protein purification device is used. Comprising a liquid inlet system, a power generation system, a filtering system and a liquid outlet system. According to the device for purifying the protein in the apple peel based on the electromembrane filtration technology, the membrane pollution degree in the filtration process is effectively reduced, the service life of a membrane is prolonged, and the membrane filtration effect is improved; according to the method for purifying the protein in the apple peel based on the electromembrane filtration technology, the membrane filtration purification speed is effectively increased, and the purity of the protein is improved; according to the method, protein nutrients in the apple peel are selectively purified by utilizing low-voltage direct-current membrane filtration, and protein and other nutrients in the apple peel are selectively separated in a high-precision manner.

Description

technical field [0001] The invention relates to the technical field of protein purification, in particular to a protein purification device and method based on electric membrane filtration technology. Background technique [0002] Apple peel is a low-cost and rich source of nutrients. Protein substances are beneficial to human health, especially the growth and development of adolescents, the prenatal and postnatal care of pregnant women, and the health and longevity of the elderly. [0003] At present, the main protein purification methods mainly include adsorption chromatography, precipitation, crystallization and membrane filtration. Among them, membrane filtration, especially microfiltration and ultrafiltration have been widely used to purify proteins from different substances. At present, the purification technology of protein substances only focuses on the use of conventional membrane filtration technology. For example, the invention patent CN111440226A discloses a sys...

Claims

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Application Information

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IPC IPC(8): C07K1/36C07K1/34C07K1/24
Inventor 王璐黄敬哲李宗浩
Owner JILIN UNIV
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