High performance liquid chromatography detection method for specific chromatograms of radix scutellariae raw medicinal materials and decoction pieces

A technology of high performance liquid chromatography and characteristic spectrum, which is applied in the field of high performance liquid chromatography inspection of the original medicinal materials of Scutellaria baicalensis and the characteristic spectrum of decoction pieces, can solve the problems of unstable front area, unstable retention time, unstable chromatographic conditions and the like, and achieves reliable The effect of detection means

Pending Publication Date: 2022-04-12
陕西孙思邈高新制药有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the inspection standard of Scutellaria baicalensis raw materials and decoction pieces is the first part of "Chinese Pharmacopoeia" 2020 edition, and there is no characteristic map inspection method. The retention time of scutellaria baicalensis raw materials and decoction pieces is unstable, the front area is unstable, front state, main peak and other series of chromatograms Conditions are unstable; therefore, we propose a high-performance liquid chromatography method for the characteristic maps of Scutellaria baicalensis and its decoction pieces

Method used

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  • High performance liquid chromatography detection method for specific chromatograms of radix scutellariae raw medicinal materials and decoction pieces
  • High performance liquid chromatography detection method for specific chromatograms of radix scutellariae raw medicinal materials and decoction pieces
  • High performance liquid chromatography detection method for specific chromatograms of radix scutellariae raw medicinal materials and decoction pieces

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] see figure 1 Shown: the present invention is a high-performance liquid chromatography inspection method for the characteristic spectrum of the original medicinal material of Scutellaria baicalensis and its decoction pieces, wherein, in the chromatographic conditions and system suitability test, octadecylsilane bonded silica gel is used as filler; acetonitrile is used as mobile phase A , use 0.1% phosphoric acid solution as mobile phase B to carry out gradient elution according to the regulations in the table; flow rate: 1.0ml / min

[0031] Detection wavelength: 280nm, column temperature: 30°C, and the number of theoretical plates calculated based on baicalin should not be less than 5000.

[0032]

[0033]

[0034] Reference solution preparation

[0035] Take 0.1g of Scutellaria baicalensis reference medicinal material, put it in a stoppered Erlenmeyer flask, add 25ml of 70% methanol, seal it tightly, ultrasonically treat it (power 250W, frequency 40kHz) for 20 min...

Embodiment 2

[0042] Precision inspection

[0043] The same batch of Scutellaria baicalensis test solution was taken, and samples were injected continuously for 6 times, and the retention time and peak area of ​​the characteristic spectrum were investigated respectively. The results showed that the RSDs of the retention time and peak area of ​​each common peak were all <3.0%, indicating that the instrument precision good.

[0044] Precision experiment results (n=6)

[0045] Experimental results of precision (n=6)

[0046]

[0047] Repeated inspection

[0048] Repeatability experiment results (n=6)

[0049] Repeatable experimental results (n=6)

[0050]

[0051] 18 batches of medicinal materials inspection results

[0052] Retention time in 18 batches of Scutellaria baicalensis

[0053] Relative retention time in 18 batches of Radix Paeoniae Alba

[0054]

[0055]

[0056] Relative retention time in 18 batches of Scutellaria baicalensis

[0057] Relative retention time in...

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Abstract

The invention discloses a high performance liquid chromatography detection method for specific chromatograms of radix scutellariae raw medicinal materials and decoction pieces, and relates to the technical field of medicinal material detection. The method takes octadecylsilane chemically bonded silica as a filler, acetonitrile as a mobile phase A and a 0.1% phosphoric acid solution as a mobile phase B, and comprises the following elution steps: step S1, gradient elution time is 0-2 minutes, 2% of the mobile phase A is taken, and 98% of the mobile phase B is taken; s2, gradient elution is carried out for 2-5 min, the mobile phase A accounts for 2-17%, and the mobile phase B accounts for 98-83%; s3, gradient elution is carried out for 5-10 min, the mobile phase A accounts for 17%-25%, and the mobile phase B accounts for 83%-75%; s4, gradient elution lasts for 10-20 min, the mobile phase A accounts for 25%, and the mobile phase B accounts for 75%; s5, gradient elution is carried out for 20-25 min, the mobile phase A accounts for 25-45%, the mobile phase B accounts for 75-55%, and the like. The high performance liquid chromatography detection method makes up the vacancy that no detection method for the specific chromatogram of the radix scutellariae raw medicinal material and the decoction pieces exists at present.

Description

technical field [0001] The invention relates to the technical field of medicinal material inspection, in particular to a high-performance liquid chromatography inspection method for characteristic maps of raw medicinal materials of Scutellaria baicalensis and decoction pieces. Background technique [0002] Scutellaria baicalensis, also known as camellia root and soil gold tea root, is a perennial herb of the Lamiaceae Scutellaria genus. Scutellaria baicalensis is a traditional Chinese herbal medicine in China. Big; [0003] At present, the inspection standard of Scutellaria baicalensis raw materials and decoction pieces is the first part of "Chinese Pharmacopoeia" 2020 edition, and there is no characteristic map inspection method. The retention time of scutellaria baicalensis raw materials and decoction pieces is unstable, the front area is unstable, front state, main peak and other series of chromatograms Conditions are unstable; therefore, we propose a high performance li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74
Inventor 王光磊何亮张仪华
Owner 陕西孙思邈高新制药有限公司
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