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High performance liquid chromatography detection method for characteristic chromatograms of sophora flower raw medicinal materials and decoction pieces

A technology of high performance liquid chromatography and characteristic map, which is applied in the field of high performance liquid chromatography inspection of the original medicinal materials of Sophora japonica and the characteristic map of decoction pieces, can solve the problems of unstable frontal area, unstable chromatographic conditions, unstable retention time, etc. The effect of reliable detection means

Pending Publication Date: 2022-04-19
陕西孙思邈高新制药有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the inspection standard of the raw medicinal materials and decoction pieces of Sophora japonica is the first part of the "Chinese Pharmacopoeia" 2020 edition, and there is no characteristic map inspection method for the time being. The series of chromatographic conditions are unstable and have certain limitations; therefore, we propose a high-performance liquid chromatography test method for the characteristic maps of the original medicinal materials and decoction pieces of Sophora japonica

Method used

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  • High performance liquid chromatography detection method for characteristic chromatograms of sophora flower raw medicinal materials and decoction pieces
  • High performance liquid chromatography detection method for characteristic chromatograms of sophora flower raw medicinal materials and decoction pieces
  • High performance liquid chromatography detection method for characteristic chromatograms of sophora flower raw medicinal materials and decoction pieces

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] see figure 1 Shown: the present invention is the high-performance liquid chromatography inspection method of the characteristic map of Sophorae japonica original medicinal materials and decoction pieces, wherein, chromatographic conditions and system applicability test:

[0030] Use octadecylsilane bonded silica gel as filler (column length 150mm, inner diameter 4.6mm, particle size 5.0μm); acetonitrile as mobile phase A, 0.1% phosphoric acid solution as mobile phase B, as shown in the following table Gradient elution was carried out according to the regulations; the detection wavelength was 257nm; the flow rate was 0.8ml per minute; the column temperature was 30°C. The number of theoretical plates should not be less than 2000 based on the rutin peak.

[0031]

[0032] Preparation of reference solution:

[0033] Take 0.1g of Sophora japonica reference medicinal material, put it in a stoppered Erlenmeyer flask, accurately add 50ml of methanol, weigh it, ultrasonical...

Embodiment 2

[0041] Take the same batch of Sophora japonica for the test solution, inject 6 times continuously, and investigate the retention time and peak area of ​​the characteristic spectrum respectively. The results show that the RSDs of the retention time and peak area of ​​each common peak are less than 3.0%, indicating that the instrument is precise. good degree.

[0042] Precision experiment results (n=6)

[0043] Experimental results of precision (n=6)

[0044]

[0045]

Embodiment 3

[0047] Repeated inspection

[0048] Repeatability experiment results (n=6)

[0049] Repeatable experimental results (n=6)

[0050]

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Abstract

The invention discloses a high performance liquid chromatography detection method for characteristic chromatograms of sophora flower raw medicinal materials and decoction pieces, and relates to the technical field of medicinal material detection. The method takes octadecylsilane chemically bonded silica as a filler, acetonitrile as a mobile phase A and a 0.1% phosphoric acid solution as a mobile phase B. The method comprises the following steps: step S1, gradient elution time is 0-28 minutes, the mobile phase A accounts for 10-18%, and the mobile phase B accounts for 90-82%; s2, gradient elution time is 28-48 min, the mobile phase A accounts for 18%-35%, and the mobile phase B accounts for 82%-65%; s3, carrying out gradient elution for 48 to 56 minutes, taking 35 to 50 percent of the mobile phase A and 65 to 50 percent of the mobile phase B; s4, gradient elution is carried out for 56-60 min, the mobile phase A accounts for 50%-10%, and the mobile phase B accounts for 50%-90%; and S5, gradient elution is carried out for 60-65 min, the mobile phase A accounts for 10%, and the mobile phase B accounts for 90%. The method effectively makes up the vacancy that no sophora flower raw medicinal material and decoction piece characteristic spectrum detection method exists in the country at present.

Description

technical field [0001] The invention relates to the technical field of medicinal material inspection, in particular to a high-performance liquid chromatography inspection method for characteristic maps of original medicinal materials and decoction pieces of Sophora japonica. Background technique [0002] Sophora japonica is a traditional Chinese medicinal material in China. It has the functions of cooling blood to stop bleeding, clearing liver and purging fire, and is widely used in clinical practice with a large amount; [0003] At present, the inspection standard of the raw medicinal materials and decoction pieces of Sophora japonica is the first part of the "Chinese Pharmacopoeia" 2020 edition. There is no characteristic map inspection method for the time being. The series of chromatographic conditions are unstable and have certain limitations; therefore, we propose a high-performance liquid chromatography detection method for the characteristic maps of the original medic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 王光磊何亮张仪华
Owner 陕西孙思邈高新制药有限公司
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