Three-dimensional reconstruction method based on single phase diagram

Abstract

The invention provides a three-dimensional reconstruction method based on a single phase diagram, which comprises the following steps: constructing a training data set and a test data set, the training data set is composed of single phase diagrams of polystyrene microsphere samples processed in Olympus lens oil, sodium chloride solutions with different concentrations and ultrapure water environment liquid, and the test data set is composed of single phase diagrams of polystyrene microsphere samples processed in the Olympus lens oil, sodium chloride solutions with different concentrations and ultrapure water environment liquid; the test data set is single phase diagram data of polystyrene microsphere samples and red blood cell samples which do not appear in the training data set; and importing the training data set into the machine learning model for training, and predicting by using the test data set to obtain the three-dimensional structure morphology of the substructure biological cells. The method solves the problems of huge data volume, too long time consumption, complex implementation process and the like in the prior art.

Description

technical field [0001] The invention belongs to the technical field of phase microscopic imaging, and in particular relates to a fast three-dimensional shape reconstruction method of a phase body based on a single phase image and machine learning. Background technique [0002] Biological cells are the basic units of organism structure and life activities. The morphological structure of the cell is closely related to the physiological activities and functions of the cell. For example, the morphological structure of the cell will change in the process of proliferation, division, metabolism and canceration. state. Therefore, the study of cell morphology is of great significance in life, health and cell biology. However, most cells are colorless and transparent, and are phase objects, which are difficult to image with traditional microscopes. Quantitative Phase Microscopy (QPM) technology can modulate the non-directly visible phase information in light waves into amplitude in...

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Problems solved by technology

[0003] Aiming at the deficiencies in the prior art, the present invention provides a three-dimensional reconstruction method based on a single phase image, which overcomes the shortcomings of the existing phase volume three-dimensional reconstruction method, such as huge data volume, long time-consuming, complex implementation process, etc.

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