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Antiviral use of progesterone

A virus and application technology, applied in the direction of antiviral agents, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problems of long research and development cycle and high cost

Pending Publication Date: 2022-05-27
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To develop different drugs for different viruses, the development cycle is long and the cost is high

Method used

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  • Antiviral use of progesterone
  • Antiviral use of progesterone
  • Antiviral use of progesterone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 In vivo and in vitro toxicity detection of progesterone

[0048] 1. Experimental materials

[0049] 1.1 Cells, Compounds, Kits

[0050] PK15 (porcine kidney epithelial cells) and 3D4 / 21 (porcine alveolar macrophages) cells were purchased from the American Type Culture Collection (ATCC); CCK kit (ZP328) was purchased from Beijing Zhuangmeng International Biogene Technology Co., Ltd. ; Progesterone (Progesterone) was purchased from SIGMA company. Balb / C mice (purchased from Zhengzhou University), infusion set needles, 4% PFA, 1×PBS, needles for limb fixation (4 pieces), 2 scissors, 1 medium blunt forceps, 1 small forceps Hand, alcohol watering can, 2 30ml syringes, ether for anesthesia.

[0051] 1.2 Experimental instruments

[0052] Varioskan Flash Spectral Scanning Multi-Reader (Thermo Fisher, USA); Fluorescence Microscope (OLYMPUS).

[0053] 1.3 Experimental reagent formula

[0054] a) Hematoxylin staining solution

[0055] Hematoxylin 2.0g ...

Embodiment 2

[0089] Example 2. Progesterone significantly inhibits the proliferation of enveloped virus

[0090] 1 Experimental material

[0091]The vesicular stomatitis virus VSV-GFP was donated by Mr. Li Yongtao from Henan Agricultural University; the pseudorabies virus PRVHN1201 was donated by Mr. Tian Kegong from Pleco Bioengineering Co., Ltd.; the porcine reproductive and respiratory syndrome virus PRRSVBJ-4 was donated by Northwest Agriculture and Forestry University; Pseudorabies virus PRV-GFP was donated by Wuhan Virus; ectromeliavirus (ECTV) was isolated from disease materials in our laboratory; ADV5-GFP was purchased from Hanheng Biotechnology Co., Ltd.; PK15 and Vero cell lines were purchased from American models Culture Collection Center (ATCC).

[0092] 2 Experimental instruments

[0093] Amersham Imager 600 colorimeter (GE Healthcare); LSM800 laser confocal microscope (ZEISS); Beckman CytoFLEX flow cytometer.

[0094] 3 Experimental methods and results

[0095] 3.1 Virus ...

Embodiment 3

[0139]Embodiment 3, progesterone significantly inhibits the entry of PRV virus

[0140] 1. Experimental materials

[0141] 1.1 Cells, Compounds, Kits

[0142] Cell-LightTM EdU Apollo567 (C10338-1) kit was purchased from Ribo Biotechnology Co., Ltd.; CellMaskTM plasma membrane dye (C37608) was purchased from Molecular Probes; Mouse anti-PRV gE antibody was purchased by Tian Kegong, Pleco Bioengineering Co., Ltd. Gift from teacher; absolute quantitative standard plasmid gH was constructed by our laboratory; Mouse anti-actin antibody was purchased from Wuhan Sanying Biotechnology Co., Ltd.; Cell Genome Extraction Kit was purchased from Laifeng Biotechnology Co., Ltd.

[0143] 2 Experimental instruments

[0144] QuantStudioTM6 Flex real-time fluorescence quantitative PCR instrument (Thermo Fisher, USA); AmershamImager 600 colorimeter (GE Healthcare); LSM800 laser confocal microscope (ZEISS).

[0145] 3 Experimental methods and results

[0146] 3.1 EdU-labeled viral genome to d...

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PUM

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Abstract

The invention relates to application of progesterone and structural analogues thereof in preparation of a reagent for inhibiting an envelope virus from entering cells. The invention also relates to the use of progesterone and structural analogues thereof in the preparation of a medicament for resisting envelope virus infection in a subject. The progesterone is proved to be capable of effectively inhibiting invasion of envelope viruses to cells and proliferation in the cells in a non-toxic range, so that the progesterone has a wide application prospect in the fields of related biological research and antiviral drugs in the future.

Description

technical field [0001] The present invention relates to the antiviral use of progesterone, especially its use in inhibiting the entry of enveloped viruses into cells and thereby exerting antiviral effects. Background technique [0002] Viruses are the most important pathogens that endanger humans and animals, posing serious threats to public safety and aquaculture. These viruses include not only the well-known influenza virus (Orthomyxoviridae), human herpes simplex virus (Herpesviridae), porcine reproductive and respiratory syndrome virus (Arteriviridae), but also some other families such as Rhabdoviridae , Coronaviridae, Paramyxoviridae, Poxviridae, and non-enveloped viruses have all brought great trouble to human beings. Taking the influenza virus as an example, it has been circulating in humans for more than 300 years, with an outbreak in a few years and a global pandemic in a few decades. It can lead to 250,000 to 500,000 deaths and 3 to 5 million severe cases per yea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/57A61P31/14A61P31/16A61P31/22
CPCA61K31/57A61P31/14A61P31/16A61P31/22
Inventor 褚贝贝王江杨国宇苏冰倩钟远
Owner HENAN AGRICULTURAL UNIVERSITY
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