Portable device for simultaneously detecting glucose and insulin as well as preparation and detection methods

A portable device, insulin technology, used in measuring devices, instruments, scientific instruments, etc.

Pending Publication Date: 2022-06-10
汪开继
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a wearable portable device capable of simultaneously detecting glucose and insulin and its preparation and detection method, which can solve the existing continuous glucose The detection system can only detect the deficiency of glucose technology alone, and realize the wearable real-time and rapid simultaneous detection of glucose and insulin

Method used

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  • Portable device for simultaneously detecting glucose and insulin as well as preparation and detection methods
  • Portable device for simultaneously detecting glucose and insulin as well as preparation and detection methods
  • Portable device for simultaneously detecting glucose and insulin as well as preparation and detection methods

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Experimental program
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Effect test

Embodiment Construction

[0062] The reagents and instruments used in the present invention have,

[0063] The sequence of the glucose aptamer (SEQ ID NO: 1):

[0064] 5'-HS-HS-C6-CTCTCGGGACGACCGTGTGTGTTTATCTGTAACAGTGTCCATTGTCGTCCC-MB-3';

[0065] Sequence of the insulin aptamer (SEQ ID NO: 2):

[0066] 5'-HS-HS-C6-AAAAGGTGGTGGGGGGGGTTGGTAGGGTGTCTTCT-MB-3';

[0067] The equipment used includes: electrochemical workstation (CHI660E, Shanghai), ALC-210.4 electronic analytical balance, PHS-3C pH meter, centrifuge (SIGMA-3-16PK), scanning electron microscope (SEM) (FEI Quanta 200), The TA working electrode purchased from Poten Industrial Technology Co., Ltd. (Qingdao, China), and the reference electrode were both Ag / AgCl electrodes.

[0068] A preparation method of a portable device for simultaneously detecting glucose and insulin, comprising the following steps:

[0069] A. Preparation of TA-AuNPs interface;

[0070] Drop 60 μL containing 10 mM HAuCl 4 0.5M H 2 SO 4 The solution was placed on the ...

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Abstract

The invention discloses a preparation method of a portable device for simultaneously detecting glucose and insulin, the method is based on a wearable biosensor continuous monitoring system, and the method comprises the following steps: A, preparing gold nanoparticles on a sensor interface; b, modifying an aptamer for identifying glucose on the interface of the sensor; and C, modifying an aptamer for identifying insulin on the interface of the sensor. The defect that an existing continuous glucose detection system singly detects glucose can be overcome, and portable, wearable, real-time and rapid detection of glucose and insulin is achieved.

Description

technical field [0001] The invention relates to the technical field of simultaneous rapid detection of glucose and insulin, in particular to a wearable portable device capable of simultaneous detection of glucose and insulin and a preparation and detection method. Background technique [0002] Diabetes mellitus is a metabolic disorder caused by the lack of insulin secretion or action, usually manifested as hyperglycemia. Over the years, the number of people with diabetes has continued to increase dramatically worldwide. At present, it is still clinically dependent on the detection of a single target blood glucose, and it is difficult to detect diabetes and pre-diabetes early. [0003] Insulin is a hormone produced by the pancreas that regulates glucose metabolism in the body. Insulin is essential to facilitate the entry of glucose from food into cells, where it is catabolized to produce the energy needed to make cells work properly. Unbalanced insulin levels in the body c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3277
Inventor 汪开继
Owner 汪开继
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