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Rapid propagation method of liparis nervosa through protocorm

A kind of technology of cyanobacteria and protocorm, applied in the field of cyanobacteria cultivation, can solve problems such as failure to be used for large-scale production, low seed germination rate, etc., achieve broad industrialization development prospects, short propagation cycle, and reduce costs Effect

Pending Publication Date: 2022-06-17
CHONGQING THREE GORGES VOCATIONAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Utilize the aseptic culture of wild Cerulea japonica seeds to germinate and produce clustered buds, the problem of low seed germination rate has appeared, and it is mainly used for in vitro preservation, but not for large-scale production

Method used

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  • Rapid propagation method of liparis nervosa through protocorm
  • Rapid propagation method of liparis nervosa through protocorm
  • Rapid propagation method of liparis nervosa through protocorm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] A kind of protocorm approach rapid propagation method of cyanobacteria, the steps are as follows:

[0071] (1) Selection and sterilization of explants: select cyanobacterial plants that grow well and have no pests and diseases, and pick the pods before they are mature but not cracked; rinse off the surface attachments under running tap water, and add Gently stir with a soft brush for 10 minutes in the washing liquid with detergent, rinse with tap water, and dry the surface moisture with absorbent paper; on the ultra-clean workbench, the preliminarily treated capsules are surface-sterilized with 75% ethanol for 30s, Rinse with sterile water for 3 times, disinfect with 10% sodium hypochlorite solution for 15 minutes, and then repeatedly wash with sterile water for 3 times, and use sterile filter paper to absorb the water on the surface of the fruit pods for later use;

[0072] (2) Tear off the pods after the sterilization in step (1) with sterile tweezers, shake the seeds...

Embodiment 2

[0084] A kind of protocorm approach rapid propagation method of cyanobacteria, the steps are as follows:

[0085] (1) Selection and sterilization of explants: select cyanobacterial plants that grow well and have no pests and diseases, and pick the pods before they are mature but not cracked; rinse off the surface attachments under running tap water, and add Gently stir with a soft brush for 20 minutes in the washing liquid with detergent, rinse with tap water, and dry the surface moisture with absorbent paper; on the ultra-clean workbench, the preliminarily treated capsules are surface-sterilized with 75% ethanol for 60s, Rinse with sterile water for 5 times, disinfect with 10% sodium hypochlorite solution for 25 minutes, and then repeatedly wash with sterile water for 5 times, and use sterile filter paper to absorb the water on the surface of the fruit pods for later use;

[0086] (2) Tear open the pods after sterilization in step (1) with sterile tweezers, shake the seeds in...

Embodiment 3

[0098] A kind of protocorm approach rapid propagation method of cyanobacteria, the steps are as follows:

[0099] (1) Selection and sterilization of explants: select cyanobacterial plants that grow well and have no pests and diseases, and pick the pods before they are mature but not cracked; rinse off the surface attachments under running tap water, and add Gently stir with a soft brush for 15 minutes in the washing liquid with detergent, rinse with tap water, and dry the surface moisture with absorbent paper; on the ultra-clean workbench, the preliminarily treated capsules are surface-sterilized with 75% ethanol for 45s. Rinse 4 times with sterile water, disinfect with 10% sodium hypochlorite solution for 20 minutes, then repeatedly wash with sterile water 4 times, and use sterilized filter paper to absorb the water on the surface of the fruit pods for later use;

[0100] (2) Tear open the pods after sterilization in step (1) with sterile tweezers, shake the seeds inside into...

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Abstract

The invention relates to the technical field of liparis nervosa cultivation, in particular to a rapid propagation method of liparis nervosa in a protocorm way. The method specifically comprises the following steps: (1) inoculating caesalpinia cucullata seeds into a protocorm induction culture medium, and carrying out protocorm induction culture to obtain caesalpinia cucullata protocorm; (2) inoculating the liparis nervosa protocorm into a proliferation and differentiation culture medium, and carrying out bud proliferation culture to obtain liparis nervosa proliferation buds; (3) inoculating the liparis nervosa proliferation buds into a rooting culture medium, and performing rooting culture to obtain liparis nervosa rooting seedlings; and (4) hardening and disinfecting the rooting seedlings of the liparis nervosa, and transplanting the rooting seedlings to a culture medium. According to the method, the liparis nervosa is propagated in the protocorm way, the proliferation multiple is far higher than that of rapid propagation in the existing stem callus way, proliferation and differentiation are completed in one step, the rooting rate is high, plants are robust, the growth vigor is good, the growth cycle is short, the culture medium cost is low, the cost can be obviously reduced, and the method can be used for large-scale industrial production.

Description

technical field [0001] The present invention relates to the technical field of C. japonica cultivation, in particular to a method for rapid propagation of C. japonica through the protocorm pathway. Background technique [0002] See blood blue, perennial herbal medicine, can be used for garden ornamental plants, is the whole grass of Orchidaceae (Orchidaceae) Liparis (Liparis) plant vein Liparis nervosa (Thunb.) Lindl., also known as squatty Children, lamb ear garlic, etc. Jianxueqing is a commonly used Chinese herbal medicine, which has many functions such as hemostasis and coagulation, analgesia, antibacterial and anti-inflammatory, strong antioxidant, hypoglycemic, anti-tumor and so on. The hemostatic activity of the extract of Jianxueqing is similar to that of Yunnan Baiyao. At present, the sources of cyanobacteria on the market mainly rely on wild resources. Due to excessive human excavation, the destruction of wild resources has become increasingly serious and has bee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002
Inventor 陈吉裕肖霞张文玲谢丽娜冉涌程茂
Owner CHONGQING THREE GORGES VOCATIONAL COLLEGE
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