Method for detecting erythrocyte deformability and size change

Abstract

The invention discloses a method for detecting erythrocyte deformability and size change, a micro-fluidic chip is composed of an inlet liquid storage tank, a separation track and an outlet liquid storage tank which are connected in sequence, a plurality of sub-channels are distributed in the separation track in parallel, each sub-channel is internally provided with a separation micro-column, the separation track has a multi-section structure, and the separation micro-column is arranged in the outlet liquid storage tank. And the arrangement of the separation micro-columns in each section of separation track is based on a formula: Dc = 1.4 G (tan theta) 0.48. According to the detection method, the damage condition and deformability of the red blood cells can be evaluated within 2 minutes, the sample size is only 10 microliters, cell blockage is avoided, the detection method can be repeatedly used, and compared with a traditional detection method, the detection method is more sensitive and has extremely high practical application value.

Description

technical field [0001] The invention relates to the technical field of cell detection, in particular to a method for detecting the deformability and size change of red blood cells. Background technique [0002] Erythrocyte deformability (ED) plays an important role in fulfilling the key function of transporting oxygen and carbon dioxide between blood and tissues. Good erythrocyte deformability enables erythrocytes (usually disc-shaped with a diameter of 8 μm) to squeeze through tiny capillaries less than 2.5 μm in diameter. Impaired or lost red blood cell deformability can lead to impaired microcirculation, altered blood viscosity, or localized thrombus formation. Thus, impaired or loss of red blood cell deformability is often associated with the pathology of many diseases, such as sepsis, cardiovascular disease and many other complications. Since the discovery of the clinical importance of ED, red blood cell deformability has been the focus of numerous studies for rapid d...

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Problems solved by technology

[0003] The conventional techniques used to characterize the deformability of red blood cells in the related art include micropipette, atomic force microscope and optical tweezers, etc., all of which are only suitable for single-cell measurement, and their measurement is limited by throughput The amount is limited, the instrument is expensive and the detection speed is relatively slow, inefficient and laborious

Hemofiltration techniques can be used to infer the deformability of red blood cell suspensions from the flow rate, and cytometry can be used to measure the elongation of red blood cells under shear stress in whole blood or red blood cell suspensions, but these measurement methods are limited by the minimum sample size and cannot Reveal information at the single-cell or subpopulation level

Ultrasonic technology has the ability to analyze the biophysical characteristics of cells, but it needs to pretreat the sample with the help of microbeads or carbon nanotubes to make them attach to the target cells, and the detection cost and difficulty are high

However, although some conventional microfluidic technologies based on microchips can meet the requirements of small sample volumes, they require expensive equipment such as high-speed cameras for auxiliary detection, and are extremely prone to equipment clogging. Filtration to remove large erythrocyte clusters, but interference from white blood cells (WBC) is unavoidable in sample analysis, and if the device is once clogged with WBCs, the observed RBC deformability is significantly reduced, leading to falsely assumed reduced RBC deformability , so a sample pretreatment step is required which greatly limits its precision

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