Method for simultaneously detecting sibutramine and fenfluramine in weight-losing health-care products

A qualitative detection and sample technology, applied in Raman scattering, material excitation analysis, etc., can solve the problems of being unsuitable for large-scale screening on site, cumbersome operation, long detection time, etc., and achieve the effect of a fast and convenient detection method

Pending Publication Date: 2022-07-22
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, the detection methods for prohibited substances added in health products are mainly TLC method, chromatography, etc. The above methods have problems such as cumbersome operation and long detection time, and are not suitable for large-scale on-site screening. Therefore, it is necessary to establish a fast and accurate method. Methods of Screening for Prohibited Additives

Method used

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  • Method for simultaneously detecting sibutramine and fenfluramine in weight-losing health-care products
  • Method for simultaneously detecting sibutramine and fenfluramine in weight-losing health-care products
  • Method for simultaneously detecting sibutramine and fenfluramine in weight-losing health-care products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] A method for qualitatively detecting sibutramine and fenfluramine in weight loss health care products, comprising the following steps:

[0056] (1) Preparation of Raman-enhanced substrate:

[0057] Soak the glassware (round-bottomed flask) and rotor used in aqua regia for 12 hours, take out and rinse with water until tasteless, rinse with ultrapure water three times, and dry in an oven at 60°C for later use; the preparation mass fraction is 1% trisodium citrate Aqueous solution, 10mg / mL potassium chloroaurate solution, for use; heating the oil bath to 120°C and keeping it constant; setting the parameters of the magnetic stirrer: 565r / min;

[0058] ①Add 47mL of ultrapure water and 3mL of potassium chloroaurate to the round-bottomed flask and mix thoroughly; put the round-bottomed flask into a 120°C oil bath, while stirring with a magnetic stirrer and keep the temperature constant until the solution boils; add 2mL The 1% trisodium citrate aqueous solution continued to be...

Embodiment 2

[0077] The volume ratio of the mixed solution and Au@ZIF-8 in Example 1 was adjusted to 1:1, 1:2, 1:3, 1:4, and the others were the same as those in Example 1, and the detection was carried out.

[0078] The test results are as follows: the gold sol is mixed with the liquid to be tested in different proportions, and the results are as follows: Figure 5 As shown, when the volume of gold sol and the liquid to be tested is 1:2, the signal intensity is the highest.

Embodiment 3

[0080] The mixing time of the mixed solution and Au@ZIF-8 in Example 1 was adjusted to 1min, 2min, 3min, and 4min, and the others were the same as those in Example 1, and the detection was carried out.

[0081] The test results are: Au@ZIF-8 is mixed with the liquid to be tested at different times, and the results are as follows Image 6 As shown, the signal intensity was the highest when the mixing time was 2 min.

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Abstract

The invention discloses a method for simultaneously detecting sibutramine and fenfluramine in weight-losing health care products, and belongs to the field of food detection. According to the method disclosed by the invention, the Raman characteristic peaks of sibutramine and fenfluramine which exist at the same time are determined; then pretreating a weight-losing health care product sample, and extracting by adopting an extraction solvent to obtain a solution to be detected; and judging whether sibutramine and fenfluramine exist in the to-be-detected sample or not according to the Raman spectrum of the to-be-detected solution. According to the method disclosed by the invention, the lowest detection concentration of sibutramine and fenfluramine in the weight-losing health-care product is 25mg/kg, qualitative analysis can be carried out on existence of sibutramine and fenfluramine in the weight-losing health-care product, and the detection time of a single sample is controlled within 2min.

Description

technical field [0001] The invention relates to a method for simultaneously detecting sibutramine and fenfluramine in weight loss health care products, and belongs to the field of food detection. Background technique [0002] In recent years, with the continuous improvement of living standards, the problem of obesity has become increasingly serious, which has plagued many people. Based on this mentality, many businesses have launched weight loss health care products that are favored by consumers. However, driven by interests, some businesses often add substances prohibited by the state to their weight loss health care products. Get more room for profit. At present, the main substances added by illegal businesses in weight loss health care products are sibutramine and fenfluramine. Among them, sibutramine is a drug that acts on the serotonin and norepinephrine pathways, which can enhance the Satiety in the human body; Fenfluramine is also a drug that acts on serotonin, whic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 董祥辉谢云飞姚卫蓉
Owner JIANGNAN UNIV
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