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Bacillus subtilis for preparing biopesticide and application thereof

A Bacillus subtilis and biological pesticide technology, applied in the field of Bacillus subtilis, can solve the problems of increasing the COD and BOD values ​​of water quality, wasting resources, polluting the environment, etc., and achieves the effects of good effect, increased production cost and low energy consumption

Pending Publication Date: 2022-08-09
南宁市拜欧生物工程有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies of the prior art, the present invention provides a Bacillus subtilis for preparing biological pesticides and its application, which solves the problem that most food processing enterprises directly discharge the waste water generated in the process of rice flour processing from the sewer, which directly increases the water quality. COD and BOD values, both pollute the environment and waste resources

Method used

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  • Bacillus subtilis for preparing biopesticide and application thereof
  • Bacillus subtilis for preparing biopesticide and application thereof
  • Bacillus subtilis for preparing biopesticide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] In this example, the inoculation ratio and temperature of Bacillus subtilis were investigated, and the seed liquid of Bacillus subtilis with different inoculation ratios was respectively inserted into the fermentation medium to carry out fermentation experiments, and the inoculation ratios were 1%, 3%, 5%, 7%, and 9%, the colony count of the liquid fermentation broth was determined, and the results are shown in Table 1 and Table 2.

[0026] Table 1 Effects of different bacterial species ratios on fermentation products

[0027]

[0028] Table 2 Effects of different fermentation temperatures on various physical and chemical indicators of fermentation products

[0029]

[0030]

Embodiment 2

[0032] The invention investigates the antibacterial test of the biological pesticide on bacteria and fungi, and the antibacterial effect is obvious, and the results are as follows.

[0033] Table 3 Bacteriostatic test of biopesticides against bacteria and fungi

[0034]

Embodiment 3

[0036] The invention investigates the bacteriostatic test of biological pesticides on crop pathogens, and the bacteriostatic effect is obvious, and the results are as follows.

[0037] Table 4 Bacteriostatic test of biological pesticides against pathogenic bacteria

[0038]

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Abstract

The invention relates to the technical field of biopesticide, and discloses bacillus subtilis for preparing biopesticide and application of the bacillus subtilis, and the bacillus subtilis has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 22878 and is preserved in Beijing. The bacillus subtilis for preparing the biopesticide and the application of the bacillus subtilis, the innovation of a fermentation strain, and the advantages of short production period, simple process, low energy consumption and the like of selecting the bacillus subtilis to produce and prepare the biopesticide, and compared with a traditional liquid fermentation method, the strain can utilize rice flour wastewater for fermentation, so that the cost is greatly reduced, and the production cost is reduced. The effect is good, efficient and energy-saving; the production cost is not required to be increased, pollution and toxic effects caused by chemical pesticides are overcome, pollution-free production, safety and environmental protection are realized, and the problems that wastewater generated in the rice noodle processing process is directly discharged from a sewer by most of food processing enterprises nowadays, COD and BOD values of water are directly increased, the environment is polluted, and resources are wasted are solved.

Description

technical field [0001] The invention relates to the technical field of biological pesticides, in particular to Bacillus subtilis for preparing biological pesticides and applications thereof. Background technique [0002] At present, the control methods of plant diseases are mainly the cultivation of disease-resistant varieties and the control of chemical pesticides. However, due to the excessive use of chemicals for a long time, it has brought serious pollution to the ecological environment and a huge threat to human health. For the development of green agricultural products and the development of sustainable agriculture, we have widely adopted pollution-free biological pesticides. Therefore, in recent years, biological methods have become more and more widely used for biological control. At present, biotechnology is used to reduce problems such as pests and diseases and difficulties in crop growth, which will be the most effective and potential way of biological control. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/125
CPCC12N1/20Y02A50/30
Inventor 苏智兴韦朝英周志强李从韦函忠侯守海陆迪卢运琨
Owner 南宁市拜欧生物工程有限责任公司
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