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Adjustable controllable tumor living vaccine and its preparation method

A live vaccine and tumor technology, applied in the field of tumor live vaccine, tumor live vaccine and preparation thereof, can solve the problems of reducing vaccine antigenicity, transient, weak immune effect, etc.

Inactive Publication Date: 2005-12-21
THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This also reduces the antigenicity of the vaccine, and the immune effect stimulated by the body is weaker and shorter than that formed by live tumor vaccines.

Method used

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  • Adjustable controllable tumor living vaccine and its preparation method
  • Adjustable controllable tumor living vaccine and its preparation method
  • Adjustable controllable tumor living vaccine and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Mononuclear cells were isolated from the femoral bone marrow of F344 rats, red blood cells were dissolved in double distilled water, inoculated into 24-well plates, mrGM-CSF and mrIL-4 were added to each well, half of the medium was changed every other day, and cultured for 8-10 days, each Rats can obtain dendritic cells 5~10×10 7 ;Typical colony growth of dendritic cells was observed under an inverted microscope; Fluorescence-labeled antibody flow cytometry was used for dendritic cell phenotype identification, and the results showed that the cell surface antigens OX6, B7-2, OX62 and ICAM were cultured for 6 days -1 were 20.89%, 22.68%, 21.93% and 13.74%, respectively; increased to 63.46%, 52.79%, 75.35% and 30.43% on day 10. The dendritic cells of F344 rats cultured on the 6th day can slightly stimulate the proliferation of T cells, with a stimulation index of 1.21±0.23; while the ability of the dendritic cells cultured on the 9th day to promote T cell proliferation wa...

Embodiment 2

[0038] Mononuclear cells were isolated from the femoral bone marrow of F344 rats, red blood cells were dissolved in double distilled water, inoculated in 24-well plates, mrGM-CSF and mrIL-4 were added to each well, and half of the medium was changed every other day, and cultured for 8-10 days, each Rats can obtain dendritic cells 5~10×10 7 ; Typical dendritic cells grew into colonies under an inverted microscope; Gimsa staining, immunohistochemistry, scanning, and transmission electron microscopy showed typical dendritic cell morphology. Fluorescence-labeled antibody flow cytometry was used to identify the phenotype of dendritic cells. The results showed that the surface antigens of dendritic cells were lowly expressed when they were immature: 45.22% for OX6 (MHC class II), and 32.76% for B7-2; high expression when mature OX6 is 89.21%, and B7-2 is 56.33%. The suicide gene CD was transfected into the F344 rat ovarian cancer cell line NUTU-19 using adenovirus as a carrier, and...

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Abstract

The invention belongs to the field of biological technology, and relates to a live tumor vaccine whose safety and immune effect can be regulated and a preparation method thereof. The present invention introduces the suicide gene system into the live tumor vaccine, and the vaccine is a live vaccine when it enters the body. After completing the function of the live vaccine, it can be controlled by the suicide gene and die, which prevents the adverse effects caused by the continuous growth of the live vaccine in the body. It can also disintegrate like ordinary vaccine cells and completely release immune substances. The results of animal experiments show that the present invention makes tumors occur later in experimental animals, smaller in size, and significantly prolongs the survival period of experimental animals, which proves that the vaccine of the present invention has a protective effect against tumor attack and can regulate the safety and immune effect of live tumor vaccines.

Description

technical field [0001] The invention belongs to the field of biological technology and relates to a live tumor vaccine, in particular to a live tumor vaccine whose safety and immune effect can be regulated and a preparation method thereof. Background technique [0002] Studies have reported that the main reasons why tumor cells can evade the surveillance of the host immune system are: (1) the low immunogenicity of tumor cells themselves; (2) the low expression of various molecules required by tumor cells to stimulate T cell responses , such as: MHC molecules, co-stimulatory molecules (B7) and adhesion molecules (ICAM-1), etc.; (3), functional defects of antigen-presenting cells. Effective presentation of tumor antigens is the key to anti-tumor immunity. [0003] The proposal of cancer vaccine (Cancer Vaccine) has a history of more than 100 years. Various forms of tumor vaccines prepared in vitro and injected into tumor patients can sensitize and activate T lymphocytes in t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00A61K48/00A61P35/00
Inventor 徐丛剑康玉
Owner THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV