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Adjustable controllable tumor eiving vacine and its preparation method

A technology of live vaccines and tumors, applied in the field of live tumor vaccines, live tumor vaccines and their preparation, can solve the problems of weak and short-lived immune effects, reducing vaccine antigenicity, etc.

Inactive Publication Date: 2004-02-25
THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This also reduces the antigenicity of the vaccine, and the immune effect stimulated by the body is weaker and shorter than that formed by live tumor vaccines.

Method used

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  • Adjustable controllable tumor eiving vacine and its preparation method
  • Adjustable controllable tumor eiving vacine and its preparation method
  • Adjustable controllable tumor eiving vacine and its preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Mononuclear cells were isolated from the femoral bone marrow of F344 rats, red blood cells were dissolved in double distilled water, inoculated in 24-well plates, mrGM-CSF and mrIL-4 were added to each well, and half of the medium was changed every other day, and cultured for 8-10 days, each Rats can obtain dendritic cells 5~10×10 7 ;Typical colony growth of dendritic cells was observed under an inverted microscope; Fluorescence-labeled antibody flow cytometry was used for dendritic cell phenotype identification, and the results showed that the cell surface antigens OX6, B7-2, OX62 and ICAM were cultured for 6 days -1 were 20.89%, 22.68%, 21.93% and 13.74%, respectively; increased to 63.46%, 52.79%, 75.35% and 30.43% on day 10. The dendritic cells of F344 rats cultured on the 6th day can slightly stimulate the proliferation of T cells, with a stimulation index of 1.21±0.23; while the ability of the dendritic cells cultured on the 9th day to promote T cell proliferation ...

Embodiment 2

[0047] Mononuclear cells were isolated from the femoral bone marrow of F344 rats, red blood cells were dissolved in double distilled water, inoculated in 24-well plates, mrGM-CSF and mrIL-4 were added to each well, and half of the medium was changed every other day, and cultured for 8-10 days, each Rats can obtain dendritic cells 5~10×10 7 ; Typical dendritic cells grew into colonies under an inverted microscope; Gimsa staining, immunohistochemistry, scanning, and transmission electron microscopy showed typical dendritic cell morphology. Fluorescence-labeled antibody flow cytometry was used to identify the phenotype of dendritic cells. The results showed that the surface antigens of dendritic cells were lowly expressed when they were immature: 45.22% for OX6 (MHC class II), and 32.76% for B7-2; high expression when mature OX6 is 89.21%, and B7-2 is 56.33%. The suicide gene CD was transfected into the F344 rat ovarian cancer cell line NUTU-19 using adenovirus as a carrier, and...

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Abstract

The present invention relates to a tumor live vaccine whose safety and immune effect can be regulated and controlled and its preparation method. The suicide gene system can be introduced into tumor live vaccine, when said vaccine is come into the body, it is a life vaccine, after the action of said life vaccine is completed, it can be controlled by suicide gene and is died. The animal tests show that said invented vaccine has protection action for antitumor aggression, and can regulate and control safety and immune effect of tumor life vaccine.

Description

technical field [0001] The invention belongs to the field of biological technology and relates to a live tumor vaccine, in particular to a live tumor vaccine whose safety and immune effect can be regulated and a preparation method thereof. Background technique [0002] Studies have reported that the main reasons why tumor cells can evade the surveillance of the host immune system are: (1) the low immunogenicity of tumor cells themselves; (2) the low expression of various molecules required by tumor cells to stimulate T cell responses , such as: MHC molecules, co-stimulatory molecules (B7) and adhesion molecules (ICAM-1), etc.; (3), functional defects of antigen-presenting cells. Effective presentation of tumor antigens is the key to anti-tumor immunity. [0003] The proposal of cancer vaccine (Cancer Vaccine) has a history of more than 100 years. Various forms of tumor vaccines prepared in vitro and injected into tumor patients can sensitize and activate T lymphocytes in t...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61K48/00A61P35/00
Inventor 徐丛剑康玉
Owner THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV