Use in clinical diagnosis of gonococcus by one section of gonococcus DNA sequence

A DNA sequence and clinical diagnosis technology, applied in the field of biomedicine, to achieve the effect of fast and accurate detection method and low cost

Inactive Publication Date: 2005-12-21
昆明云大生化科技有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Accurate diagnosis is the basis for immediate and reliable treatment, currently only based on clinical indications

Method used

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  • Use in clinical diagnosis of gonococcus by one section of gonococcus DNA sequence
  • Use in clinical diagnosis of gonococcus by one section of gonococcus DNA sequence
  • Use in clinical diagnosis of gonococcus by one section of gonococcus DNA sequence

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Experimental program
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Effect test

Embodiment Construction

[0019] Firstly, the nucleotide sequence of Neisseria gonorrhoeae was searched in the gene database with Neisseria gonorrhoeae as the key word. A total of several closely related nucleotide sequences were obtained, and these nucleotide sequences were compared with the nucleotide sequences (nr) in the gene database using the Blast online software on the NCBI network to exclude non-specific nucleotide sequences, A 6Kb specific nucleotide sequence was obtained. More than 20 pairs of PCR primers were designed based on this sequence, DNA was extracted from clinical specimens, PCR amplification was performed, and a pair of sequences and primers suitable for PCR amplification were screened out. The products obtained by PCR amplification were consistent with the expected size (see Fig. 1. The serial numbers in the figure are: 1. Standard molecular weight DNA, the molecular weights from top to bottom are: 622, 527, 404, 309, 242 / 238, 217, 201, 190, 180, 160+160, 147+147 , 123; 2, 4, 5 ...

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Abstract

The invention relates to the application of a Neisseria gonorrhoeae DNA sequence and belongs to the technical field of biomedicine. The invention determines the specificity of a Neisseria gonorrhoeae DNA sequence and the PCR amplification primer through the search and analysis of the gene database data, and the length of the sequence is 198bp. The sequence is obtained by PCR amplification and cloning of the human genital gonococcal infection specimen, and the sequencing proves that it is consistent with the predicted sequence. The results of bioinformatics analysis and gene chip hybridization analysis confirmed the specificity of the sequence to Neisseria gonorrhoeae. The invention has the advantages of rapid and accurate detection method, simple and convenient operation, low cost and the like. The invention is used as a gene chip diagnosis probe and a PCR amplification detection sequence for the diagnosis of Neisseria gonorrhoeae.

Description

Technical field: [0001] The invention relates to the application of a DNA sequence of Neisseria gonorrhoeae and belongs to the technical field of biomedicine. Background technique: [0002] Neisseria gonorrhoeae is a pathogen that frequently infects the urinary system, which can cause urethritis, cervicitis, salpingitis, bacteremia, arthritis, etc., and can lead to spontaneous abortion, premature rupture of membranes, premature delivery and acute choroidosis during pregnancy. membrane amnionitis, and gonococcal ophthalmia, pharyngeal infections, and other syndromes in newborns. Accurate diagnosis is the basis for immediate and reliable treatment, which can only be diagnosed according to clinical indications at present. PCR and gene chip diagnosis is a fast and accurate detection method. Diagnostic methods of this type rely on the availability of specific nucleic acid sequences. content of the invention; [0003] The invention is to determine a specific nucleotide sequen...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12P19/34C12Q1/68
Inventor谭德勇朱宝生
Owner昆明云大生化科技有限责任公司