Method and composition for altering a b cell mediated pathology
A technology of B cells and compositions, applied in the field of immunology and immunotherapy, can solve problems such as changing chimeric idiotype proteins
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[0162] In the following description, reference will be made to various methods known to those skilled in the fields of immunology, cell biology, and molecular biology. Publications and other materials describing such known methods referred to are incorporated herein by reference in their entirety, as if they were described in full.
[0163] 1. Tissue processing for identification and cloning of non-Hodgkin's lymphoma idiotype (ID):
[0164] Under aseptic conditions, according to a clinically specified method, a biopsy is performed on a tumor sample obtained from a peripheral lymph node, and the sample is used to produce a patient's idiotype-specific chimeric immunoglobulin recombinant protein. Store the remaining lymph node biopsy material in the tissue cell bank in liquid nitrogen for future use.
[0165] a. Cell separation: A single cell suspension of the patient's lymph node biopsy sample is obtained by forcing the patient's biopsy lymphoma tissue to pass through a disposable 0...
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