Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Gene promoter for specific expression of plant trichome

A promoter and trichome technology, applied in the field of plant molecular biology and plant genetic engineering, can solve the problem of little similarity of nucleotide sequences, reduce physical space constraints, avoid accumulation, and facilitate high-level expression Effect

Inactive Publication Date: 2006-11-01
SUN YAT SEN UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These promoters can drive the specific expression of the GUS reporter gene in trichomes, but the nucleotide sequences of these promoters have little similarity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gene promoter for specific expression of plant trichome
  • Gene promoter for specific expression of plant trichome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Adapter-ligation PCR (Adaptor-ligation PCR) method was used for genomic DNA walking, and the promoter sequence of SaPIN2b gene was amplified and cloned from Solanum nigrum. The kit used for genome walking is GenomeWalker from Clontech TM Kit. Specific steps are as follows:

[0021] 1. Extraction of Solanum nigrum Genomic DNA

[0022] Take 5 grams of nightshade leaves, grind them into powder in liquid nitrogen, transfer them to a 30ml centrifuge tube, add 15ml of extraction buffer [100mM Tris (pH 8.0), 50mM EDTA (pH 8.0), 500mM NaCl, 10mM β-mercaptoethanol] , mix well. Then add 1 ml of 20% SDS to the tube, mix thoroughly, and incubate at 65°C for 10 minutes. Then add 5ml of 5M potassium acetate, mix thoroughly and place on ice for 20 minutes. Centrifuge at 25,000×g for 20 minutes, transfer the supernatant to another clean centrifuge tube, add 10ml of isopropanol, mix well and let stand at -20°C for 30 minutes. Centrifuge at 20,000×g for 15 minutes, remove the supe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a gene promoter specifically expressed in plant trichomes. The present invention provides the sequence of the trichome-specific solanum protease inhibitor (SaPIN2b) gene promoter that adopts genomic DNA walking method to clone; Determine the SaPIN2b transcription start site with 5' cDNA end rapid amplification method; The DNA sequence comprising the SaPIN2b promoter is fused with a β-glucuronidase (GUS) reporter gene to construct a plant expression vector; the resulting vector is used to transform tobacco and Solanum nigrum, and the obtained transgenic plants are detected, indicating that the GUS reporter Genes are specifically expressed in trichomes.

Description

technical field [0001] The present invention relates to the fields of plant molecular biology and plant genetic engineering, in particular to a gene promoter. Background technique [0002] Like other eukaryotes, the expression of plant genes is regulated at multiple levels such as DNA level, transcription level, post-transcription level, translation level and post-translation level. From the effect point of view, regulation at the transcriptional level is the most economical and effective, so transcriptional regulation is the most important way to control plant gene expression. The transcription of genes into mRNA is regulated by a certain region of the gene, and the region that regulates gene transcription before the transcription start site is usually called a promoter. The region proximal to the transcription initiation site constitutes the "core promoter region"; it typically contains the TATA box and CAAT box sequences, as well as the transcription initiation site. Th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/63C12N15/82C12N15/70
Inventor 徐增富刘进邓宇歌黄小乐
Owner SUN YAT SEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com