Use of ampelopsin in preparation of angiogenesis inhibitor drug

A kind of sapphire, inhibitor technology, applied in sapphire in the pharmaceutical field

Inactive Publication Date: 2007-05-16
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report on the application of staphylocoxine in the preparation of drugs that inhibit the synthesis and secretion of VEGF and bFGF

Method used

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  • Use of ampelopsin in preparation of angiogenesis inhibitor drug
  • Use of ampelopsin in preparation of angiogenesis inhibitor drug
  • Use of ampelopsin in preparation of angiogenesis inhibitor drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Take the root of the Vitis vinifera or Vitis vinifera, extract and purify the root of the grapevine by a conventional extraction method to prepare the viniferin, and pack it into 500mg / piece powder injection ampoules or injections.

Embodiment 2

[0016] The vine tea is routinely extracted for the extract of splendens, without purification, and after content determination, the auxiliary materials are added according to the conventional method, granulated, sieved, and the glidant magnesium sulfate and talcum powder are added; processed according to the conventional production process of tablets Into tablets, each tablet contains 250mg of snake grapevine.

Embodiment 3

[0017] Example 3 Bovine aortic endothelial cell proliferation inhibition experiment by MTT method

[0018] 3.1.1 Isolation and culture of neonatal bovine aortic endothelial cells

[0019] References [Chai Weidong, et al. Culture of fetal bovine aortic endothelial cells. Journal of Nanjing Medical University, 2000, 20(3): 173] method, the newborn healthy cows were taken and sacrificed by carotid bloodletting, and the aorta was separated under sterile conditions. Length 12-15cm, put into PBS with double antibody (penicillin, streptomycin each 100U / mL). Take out the artery on the ultra-clean bench, remove the fascia and fat from the outer wall of the artery, and use sterile PBS with pH 7.4 phosphate buffer (concentration mmol / L: NaCl130, KCl2.5, Na 2 HPO 4 10, KH 2 PO 4 1.5) Rinse repeatedly 3-5 times, and cut along the center of the petri dish. A layer of 0.25% trypsin was dropped on the inner wall of the artery, and the digestion was terminated immediately after 10-20 seco...

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Abstract

The invention relates to the use of ampelopsin in preparation of angiogenesis inhibitor drug, wherein the suppression function of ampelopsin to propagation of blood vessel endothelial cells is reviewed, the suppression function of ampelopsin to synthesis and secretion of VEGF, bFGF by human hepatoma cells is also examined, test results show that ampelopsin has suppression function to propagation of blood vessel endothelial cells, and also suppression action to the synthesis and secretion of VEGF and bFGF.

Description

technical field [0001] The invention relates to the application of staphylococcus in the field of pharmacy, in particular to the application of staphylococcus in the preparation of medicaments for vascular endothelial cell growth factor VEGF and basic fibroblast growth factor bFGF inhibitors. Background technique [0002] Vitigenin is the main component of several Viticidaceae plants (such as rhizomes, vine tea, etc.) that are widely distributed in South China. Snake grapevine, also known as dihydromyricetin, belongs to flavonoids, and its structural formula is [0003] [0004] Chemical name: 3,5,7,3',4',5'-hexahydroxy-2,3-dihydroflavone. [0005] Wuyigen is the root or whole plant of Ampelopsis cantoniensis (HooK.etArm) Planch, which is mainly produced in southern China and is a traditional folk medicine. "Dictionary of Traditional Chinese Medicine" records that it has the functions of anti-inflammatory and detoxification, and is mainly used for osteomyelitis, acute l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/353A61P35/00
Inventor 刘德育
Owner SUN YAT SEN UNIV
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