Method for preparing aftosa vaccine

A foot-and-mouth disease vaccine and a technology for vaccine preparation, which are applied in the field of animal vaccine preparation, can solve the problem of low immune efficacy, and achieve the effects of increasing virus concentration, facilitating ultra-large-scale production, and reducing the dose of immunization used.

Active Publication Date: 2007-07-25
薛景山
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, the adjuvant dosage form currently used in China is biphasic oil adjuvant, which is easy to absorb and has a faster immune response, but the immune effect is not high

Method used

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Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0017] The present invention will be described in further detail below in conjunction with specific embodiment, and these embodiments do not limit protection scope of the present invention, and all modifications and variations based on the basic idea of ​​the present invention all belong to this invention.

[0018] protection scope of the invention.

[0019] Preparation of various types of antigens:

[0020] 1. Culturing Type O Antigen

[0021] The traditional technique is to culture the monolayer cells by rotating the bottle at a speed of 10-12 rpm. After the monolayer cells are full, insert the seed virus and virus culture solution, wherein the seed virus is added at 1% of the virus culture solution, and the virus culture solution is: Hydrolyzed milk protein solution 90%, antibiotic 2%, amino acid solution 5%, calf serum 1%, 3% glutamine solution 1%, add HEPES 0.5%, add NaOH 0.05%, add NaHCO 3 0.45% with 8.8% NaHCO 3 Fine-tune the pH of the solution to 7.5±0.1;

[0022]...

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Abstract

The invention provides a method for preparing aftosa vaccine, which comprises cell culture, virus breeding and vaccine preparation, after monolayer cells are full, seed virus culture liquid is switched in, wherein the content of seed virus is 1-5% of virus culture liquid, and the virus culture liquid mainly comprises, protein hydrolysate solution 85-90%, antibiotics 0.5-2%, amino acid solution 5-10%, 3% glutamine 1%, HEPES 0.5-0.8%, adjusting the pH to 7.5+-0.1 by charging queous alkali, finally preparing vaccine through deactivating the virus and mixing.

Description

technical field [0001] The invention relates to a preparation method of an animal vaccine, in particular to a preparation method of a foot-and-mouth disease vaccine. Background technique [0002] Foot-and-mouth disease is a Class A animal epidemic disease stipulated by the International Veterinary Bureau. The research on foot-and-mouth disease vaccine has always been concerned by researchers at home and abroad. Suspension culture technology is currently used abroad to cultivate cells and viruses, which has the advantages of high automation and high quality stability. The disadvantage is that this method of cultivating viruses has a low virus content and must be concentrated by ultrafiltration before it can be used for vaccine production, which greatly limits the production of vaccines. This kind of production is only suitable for vaccine storage and small amount of immunization needs, not suitable for the large amount of immunization against foot-and-mouth disease in our co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/135A61P31/14
Inventor 薛景山
Owner 薛景山
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