Application of Vitreoscilla hematoglobin gene in improving waterlogging resistance of plant

A technology of hemoglobin and Vibrio lucidum, which is applied in the field of Vibrio lucidum hemoglobin gene, and can solve the problems of insignificant effect and the like

Inactive Publication Date: 2002-12-25
WUHAN UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, Holmberg and people never imagined that the hemoglobin gene of Vitella hyaline had a new use of waterlogging resistance in transformed plants, and this new use is exactly what this kind of plants do not have. People are looking for waterlogging resistance in their dreams. Related genes, and try to improve, improve and improve the excellent traits of crops, but there has been no harvest or the effect is not obvious

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  • Application of Vitreoscilla hematoglobin gene in improving waterlogging resistance of plant
  • Application of Vitreoscilla hematoglobin gene in improving waterlogging resistance of plant
  • Application of Vitreoscilla hematoglobin gene in improving waterlogging resistance of plant

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Embodiment 1

[0037] DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Example 1 (Application of Vibrio vibrio hemoglobin gene in tobacco waterlogging tolerance) Construction of VHB expression vector and expression cassette 1. Construction of vector

[0038] Plasmid RED2 contains VHB gene. After we extracted and purified plasmid RED2, we digested it with restriction enzyme HindIII to obtain two fragments (2.2Kb and 2.7Kb). Since there are no effective cloning restriction sites at both ends of the known VHB sequence, the BamHI and SacI restriction sites were connected to the 5' and 3' ends of VHB by the method of primer design, and the VHB gene was analyzed by PCR method. After the amplification product was recovered, it was digested with BamH / SacI and gel purified to obtain the target DNA fragment. Plasmid pBI121 was also digested with BamHI / SacI, and after dephosphorylation at the 5' end, the target DNA fragment obtained earlier was ligated with it to construct the...

Embodiment 3

[0038] Plasmid RED2 contains VHB gene. After we extracted and purified plasmid RED2, we digested it with restriction enzyme HindIII to obtain two fragments (2.2Kb and 2.7Kb). Since there are no effective cloning restriction sites at both ends of the known VHB sequence, the BamHI and SacI restriction sites were connected to the 5' and 3' ends of VHB by the method of primer design, and the VHB gene was analyzed by PCR method. After the amplification product was recovered, it was digested with BamH / SacI and gel purified to obtain the target DNA fragment. Plasmid pBI121 was also digested with BamHI / SacI, and after dephosphorylation at the 5' end, the target DNA fragment obtained earlier was ligated with it to construct the expression vector PBI121 / RED2. After introduction of Agrobacterium LBA4404, it was used for the transformation of tobacco. 2. Recombinant plasmid digestion and sequencing identification 1) PCR primer P1 (5'to 3', 29bp) for VhbCGG GAT CCA TAA TAA TGA ACT TAA GGA...

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Abstract

An application of vitreoscilla hematoglobin gene in improving the waterlogging resistance of plant is disclosed. Said gene is constructed in the expression vector, which is then transferred to plant cells to make said gene be expressed in plant cells. The resultant transgenic plant has high resistance to waterlogging.

Description

technical field [0001] The invention relates to the use of Vibrio vitreous hemoglobin gene, and more particularly relates to the use of Vibrio vitreous hemoglobin gene in waterlogging tolerance in transgenic plants. Background technique [0002] In some organisms there is an O 2 Binding protein - hemoglobin. Hemoglobin is now found in bacteria, yeast, plants and animals. All hemoglobins have one thing in common: they contain an iron-bound heme group responsible for binding to oxygen. Plant, animal and bacterial hemoglobin may have evolved from the same most primitive hemoglobin. Gene sequence analysis shows that the differentiation of plant and animal hemoglobin was about 900 million to 1.4 billion years ago. The amino acid sequence data show that Escherichia coli and yeast flavin hemoglobin are the most primitive hemoglobin found so far, and the hemoglobin of these two organisms has been differentiated at least 1.8 billion years ago. In the plan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/00
Inventor 吕应堂梁述平汪杏芬杨礼香
Owner WUHAN UNIV
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