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Sulfation algae glycan oligose

A technology of sulfated alginate oligosaccharides and sulfated alginate, which is applied in the fields of sugar production, sugar production, food science, etc.

Inactive Publication Date: 2003-06-04
TAKARA HOLDINGS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no commercially available enzyme that decomposes the sulfated polysaccharides of brown algae, and the sulfated polysaccharides of brown algae vary depending on the type of seaweed, so in order to determine the structure of a sulfated polysaccharide, a decomposing enzyme that specifically decomposes the sulfated polysaccharide is very necessary

Method used

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  • Sulfation algae glycan oligose
  • Sulfation algae glycan oligose
  • Sulfation algae glycan oligose

Examples

Experimental program
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Effect test

reference example 2

[0034] Grind 2 kg of dried longan after breeding with a cutting machine (manufactured by Masuko Sangyo Co., Ltd.) equipped with a sieve with a pore size of 1 mm, suspend in 20 liters of 80% ethanol, stir at 25°C for 3 hours, and filter with filter paper . The resulting residue was suspended in 40 liters of 30 mM phosphate buffer (pH 6.5) containing 100 mM sodium chloride, treated at 95° C. for 2 hours, and then filtered through a stainless steel sieve with a pore size of 106 μm. 200 g of activated carbon, 4.5 liters of ethanol, and 12,000 U of alginate lyase K (manufactured by Nakaze Seika Kogyo Co., Ltd.) were added to the obtained filtrate, stirred at 25° C. for 20 hours, and centrifuged. The obtained supernatant was concentrated to 4 liters with an ultrafilter equipped with a hollow fiber having an exclusion molecular weight of 100,000, centrifuged to remove insoluble matter, and left standing at 5° C. for 24 hours. The resulting precipitate was removed by centrifugation, ...

reference example 3

[0035] 7 g of the dried fucoidan described in Reference Example 1 was dissolved in 700 ml of 20 mM imidazole hydrochloride buffer (pH 8.0) containing 50 mM sodium chloride and 10% ethanol, and the insoluble matter was removed by centrifugation. The resulting supernatant was poured into a DEAE-Cellulofine A-800 column (11.4×48 cm) equilibrated with the same buffer, washed with the same buffer, and then eluted with a concentration gradient of sodium chloride from 50 mM to 1.95 M. The eluent was taken out every 250ml. The total sugar content of each part was determined by the phenol sulfuric acid method, and the uronic acid content was determined by the carbazole sulfuric acid method. Parts 43-49, 50-55, and 56-67 were sorted according to the elution order, desalted by electrodialysis and then freeze-dried to obtain 340 mg from 43-49, 870 mg from 50-55, and 56-67. 2.64 g dry matter. The fraction obtained from fractions 56-67 was used as the sulfated alginate fraction. Referenc...

Embodiment 1

[0046] In order to measure the average molecular weight of the reaction product, commercially available amylopectin (STANDARD P-82, manufactured by Showa Denko Co., Ltd.) with known molecular weight was analyzed under the same conditions as the above-mentioned HPLC analysis, and the molecular weight of amylopectin and The relationship of the retention time is used as a standard curve for determining the molecular weight of the above reaction product. In addition, protein quantification was carried out by measuring the absorbance at 280 nm of the enzyme solution. At this time, the absorbance of 1mg / ml protein solution is calculated as 1.0. Example 1 Preparation of sulfated alginate decomposing enzyme

[0047] Inoculate Fucoidan SN-1009 on the culture medium, and cultivate it for 24 hours at 25°C to become a seed culture solution, wherein the culture medium is 4ml of fucoidan prepared by the method of Reference Example 1 containing 0.2% A culture medium composed of artificial ...

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Abstract

A smaller molecule obtainable by allowing a sulfated fucan-digesting enzyme which digests a novel sulfated polysaccharide derived from an alga belonging to Laminariales to act on a sulfated fucan, and a method for producing the same.

Description

technical field [0001] The present invention relates to sulfated alginan oligosaccharides, which are very useful in the field of sugar industry, and a preparation method thereof. Background technique [0002] Brown algae contain various sulfated polysaccharides. These polysaccharides are collectively called fucoidan or fucoidan, but their structures vary depending on the source seaweed. For example, sulfated polysaccharides extracted from fucus, kelp, Okinawa mozuku, mozuku, and wakame have different structures. Therefore, in order to enzymatically decompose sulfated polysaccharides to obtain oligosaccharides or define their structures, it is necessary to obtain enzymes that decompose them. [0003] As molecular species of sulfated polysaccharides, sulfated alginate, sulfated fucoglucuronomannans, sulfated fucogalactans, etc. (Sulfated fucogalactans) are known, and There are various other molecular species. Sulfated polysaccharides generally have certain biological activ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/006C08B37/00
Inventor 酒井武余目眸河合高志児岛薰中一夫猪饲勝重加藤郁之进
Owner TAKARA HOLDINGS
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