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Composition for improving intestine and gastric action

A composition and mixture technology, applied in the direction of steroids, medical preparations containing active ingredients, organic active ingredients, etc., can solve problems affecting application, high cost, and high production cost

Inactive Publication Date: 2003-09-17
上海中科伍佰豪生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no large-scale production process and the production cost is high
[0009] At present, the industry mainly adopts chemical method to prepare chitooligosaccharides. However, the cost is high and the quality is poor. The residue of chemical substances in the products obtained by this method affects its application in food and medicine.

Method used

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  • Composition for improving intestine and gastric action
  • Composition for improving intestine and gastric action
  • Composition for improving intestine and gastric action

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Extraction and purification of isolates with inhibitory effect on Helicobacter pylori

[0085] With solid cultured Hericium erinaceus mycelium as raw material, according to figure 1 The process shown is for extraction. Wherein, the mixture of crushed mycelium and 10 times of water was refluxed at 100°C for 3 hours, 5 times in total; the mixture of filter residue and 12 times of alkaline ethanol solution (pH10) was refluxed for 3 hours, 5 times in total; The mixture of 10 times acidic ethanol (pH3-5) solution was refluxed for 3 hours, 5 times in total.

[0086] Components FrI, FrII, FrIII and FrIV are produced. The extraction rate of each component is shown in Table 1.

[0087] components

Embodiment 2

[0089] Further Separation of Component FrIII

[0090] A. Thin layer chromatography (TLC) and results

[0091] Thin-layer chromatography was carried out using the following systems as developing solvent and sulfuric acid: methanol (1:1) as developing reagent.

[0092] System 1—n-hexane:ethyl acetate (1:1)

[0093] System 2—n-hexane:ethyl acetate:ether (1:1:1)

[0094] System 3—chloroform:ether:ethyl acetate (9:1:1)

[0095] System 4—toluene: ethyl acetate: acetic acid (12:4:0.5)

[0096] The results showed that toluene: ethyl acetate: acetic acid (12:4:0.5) was the best developing solvent. There are about 10 spots in the ethanol extract of Hericium erinaceus mycelium.

[0097] B. Silica gel column chromatography conditions and results

[0098] Silica gel: 200-300 mesh

[0099] Eluent: chloroform:methanol=9:1, 8:2, 1:1

[0100] Developing agent: toluene: ethyl acetate: acetic acid = 12:4:0.5

[0101] Flow rate: rapid separation, 400ml / 15 minutes

[0102] Volume per tub...

Embodiment 3

[0106] Qualitative identification of each component or fraction

[0107] (1) FrI and FrII:

[0108] A color development method using anthrone sulfuric acid. Take a small amount of the above precipitate, dissolve it in deionized water, take 1ml of the solution, add 4ml of anthrone sulfuric acid reagent (1g of anthrone is dissolved in 500ml of concentrated sulfuric acid), boil for 10 minutes, and it turns blue-green, which proves that FrI contains polysaccharides (Hericium erinaceus polysaccharides), while FrII contains oligosaccharides.

[0109] Further analysis of FrII shows that its main component is chitooligosaccharides, the molecular weight is less than 10000 Daltons, and the chitooligosaccharides of 1500-3000 Daltons account for more than 85%.

[0110] (2) FrIII

[0111] A. Using acetic anhydride-sulfuric acid reaction (Liebermann-Burchard reaction):

[0112] Take each 1ml of the concentrated solution in Example 1, evaporate to dryness, add 1ml of acetic anhydride to ...

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Abstract

A composition for improving the function of intestine and stomach by suppressing the growth of pylorospirobacillus contains the extract of hedgehog hydnum, oligochitose and / or organic powder. The processes of preparing the said extract of hedgehog hydnum and oligochitose are also disclosed.

Description

technical field [0001] The present invention relates to a composition for inhibiting and improving the stomach, and more specifically, the present invention relates to an isolate derived from Hericium erinaceus which can be used as an inhibitor of Helicobacter pylori, and a composition containing the isolate. Background technique [0002] Since Marshall and Warren isolated Helicobacter pylori (Hp) from gastritis patients in 1982, scientists from all over the world have conducted extensive research on the biological characteristics, pathogenic mechanism, diagnosis and treatment of Helicobacter pylori, and now It is confirmed that Helicobacter pylori is the main pathogenic factor of chronic gastritis and is closely related to the pathogenesis of peptic ulcer. WHO and International Cancer Research Organization have listed it as the first type of carcinogen. [0003] Clinically, it is difficult to eradicate Helicobacter pylori infection with a single antibiotic, so different dru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/575C07J9/00C08B37/08
Inventor 李平作赵国屏陈常庆
Owner 上海中科伍佰豪生物工程有限公司
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