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Method for preparing tissue chip

A production method and technology of tissue chips, applied in biochemical equipment and methods, preparation of test samples, material inspection products, etc., can solve problems such as difficulty in making high-density and high-precision tissue chips, inconvenient sampling observation and positioning, etc. Achieve the effect of high positioning accuracy, simple method and easy promotion

Inactive Publication Date: 2003-10-01
但汉雷 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method needs to maintain the semi-melted paraffin state for a long time, and it is also inconvenient to use a microscope to observe and locate the sampling site during the sampling and spotting process, and it is difficult to make high-density, high-precision tissue chips

Method used

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  • Method for preparing tissue chip
  • Method for preparing tissue chip
  • Method for preparing tissue chip

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Embodiment Construction

[0020] The required equipment and articles for the method for making a tissue chip in a preferred embodiment include: an ordinary optical microscope and appropriate modification; a set of special punching needles, sampling needles and related components that can be installed on the microscope lens or lens holder ( Chinese invention patent application number: 03113734.2); a special paraffin block fixing box that can be installed on the microscope stage (Chinese invention patent application number: 03113733.4); the adjustment screw and nut and control Fixed card for vertical and horizontal movement of glass slide device; donor paraffin block and paraffin embedded tissue; recipient paraffin block; temperature-controlled oven; instruments, equipment and reagents related to routine tissue sectioning, staining and immunohistochemistry.

[0021] figure 1 It is a schematic diagram of refitting an ordinary microscope according to a preferred embodiment of the present invention. Its mo...

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Abstract

A process for preparing paraffin wax embedded tissue microarray chip with oridinary microscope includes such steps as installing special perforating needle and sampling needle to the lens holder of the ordinary optical microscope, putting special paraffin wax block fixing box on object carrier, turning the lens holder and regulating the distance between lens and object carrier while perforating, sample and checking sample, and observing while locating. Its advantages are simple operation and low cost.

Description

Technical field [0001] The invention relates to a method for making a biological chip, and further relates to a method for making a paraffin-embedded tissue chip. technical background [0002] Tissue microarray (tissue microarray, TMA, also known as "tissue microarray") is an important biochip technology that emerged after gene chip and protein chip. Because it can detect a large number of tissue samples at the same time, the test results are scientific It has strong comparability, large amount of information, high efficiency, and less consumption of reagents. It has broad application prospects in gene and protein research, molecular pathology research, drug screening, health and environmental monitoring, and national defense and military in the post-genome era. [0003] Making paraffin-embedded tissue arrays is one of the key technologies of tissue chips. Battifora and Wan et al. have reported that dewaxed and dehydrated tissue specimens were randomly combined and arranged...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04G01N1/28G01N1/36G01N33/53
Inventor 但汉雷张亚历
Owner 但汉雷
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