Human inflammatory mammary cancer cell lines and its establishment

A breast cancer cell, breast cancer technology, applied in the establishment of human inflammatory breast cancer cell lines and the field of establishment

Inactive Publication Date: 2004-04-21
FUDAN UNIV SHANGHAI CANCER CENT
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The success rate of human breast cancer transplantation model is only about 10%

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Human inflammatory mammary cancer cell lines and its establishment
  • Human inflammatory mammary cancer cell lines and its establishment
  • Human inflammatory mammary cancer cell lines and its establishment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Establishment of inflammatory breast cancer cell lines

[0024] 1) Primary and subculture transplantation:

[0025] The tumor tissue was aseptically excised, and the primary subcutaneous inoculation was performed.

[0026] Orthotopic transplantation: remove fiber, fat and other non-tumor tissues and necrotic parts, rinse with sterile saline, and cut the tumor into 0.5mm 3 . After intraperitoneal anesthesia, the surface skin of the mammary glands of female nude mice was disinfected with iodine wine and alcohol, and the tumor tissue was inoculated into the subcutaneous fat pad. Primary xenograft tumor grows to about 2cm 3 , the nude mice were sacrificed, and the primary xenograft tumors were excised;

[0027] Continue serial passage according to the method of primary transplantation.

[0028] 2) Primary culture of tumor cells:

[0029] Take fresh inflammatory breast cancer xenograft tumor tissue block, cut into 1mm 3 And evenly attached to 50ml Cornin  Add 5 ml o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A human inflammatory breast cancer's cell line is created by use of the transplanted tumor tissue from the naked mouse model of human inflammatory breast cancer as the tumor source and the external cutlure mode for subculturing to obtain said cell line with high transfer phynotype. Said cell line features that its cells appear as multipolar epthelial form, are collected to become flake or aggregate and adhered on wall firmly, and have the distorted number and structure of chromosomes. It can reproduce the clinic characteristics of inflammatory breast cancer.

Description

technical field [0001] The invention belongs to the field of microbial animal cell lines, and in particular relates to the establishment of a human inflammatory breast cancer cell line and its establishment method. Background technique [0002] Breast cancer is a common malignant tumor in women, and hematologic metastasis and lymphatic dissemination are the two most important metastasis routes of breast cancer. Distant metastasis is the main factor affecting the efficacy and prognosis of tumors and leading to the death of patients. Inflammatory breast cancer (Inflammatory breast cancer, IBC) is a rare special type of breast cancer with high grade and poor prognosis. "Inflammation" changes such as redness and swelling of the skin, and distant metastasis in a short period of time. The vast majority of cases had no indications for surgery when they went to the doctor, and the average survival time of patients was less than 2.5 years. [0003] At present, the molecular mechan...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12Q1/00
Inventor 邵志敏刘刚
Owner FUDAN UNIV SHANGHAI CANCER CENT
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap