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Extraction of polysaccharose of three bristle cudrania

A technology of polysaccharide and Zhe tree, which is applied in the field of extracting polysaccharides from Zhe roots, which can solve the problems of separation and purification, physical and chemical properties and structural analysis that have not yet been reported publicly.

Inactive Publication Date: 2005-02-23
ZHONGAO SCI & TECH SHANDONG LUKANG GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no public reports on the separation and purification, physical and chemical properties, and structural analysis of polysaccharides from the root of Zhessinus edulis.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The roots of Quercus tree were crushed, soaked in 6 times the volume of water for 6 hours, extracted 3 times at 70℃, extracted for 2 hours for the first time, and extracted for 1 hour each for the second and third times with water, combined the extracts, centrifuged at 4000 r / min for 15 Minutes, the sand core funnel filter. Equilibrate the 3520 adsorption column with buffer to pH=4, pass the filtrate through the 3520 column, collect the filtrate, concentrate under reduced pressure at 40°C to 1 / 3 of the original volume, centrifuge at 4000r / min for 15 minutes, collect the supernatant and add 3 times the volume 95% ethanol, stand overnight at 4°C, centrifuge at 4000r / min for 15 minutes to get the precipitate, wash the precipitate 2-3 times with 75% ethanol, absolute ethanol, ether, and acetone, centrifuge at 4000r / min The precipitate obtained in 15 minutes was dissolved in distilled water to make it fully reconstituted. The Sevag method was used to remove protein. Repeatedly u...

Embodiment 2

[0029] The roots of Quercus tree were crushed, soaked in 8 times the volume of water for 6 hours, extracted twice at 70℃, extracted for 2 hours at the first time, and extracted with water for the second time for 1 hour. The extracts were combined and centrifuged at 4000r / min for 15 minutes. Heart funnel filtration. Equilibrate the 3520 adsorption column with buffer to pH=4, pass the filtrate through the 3520 column, collect the filtrate, concentrate under reduced pressure at 40°C to 1 / 3 of the original volume, centrifuge at 4000r / min for 15 minutes, collect the supernatant and add 3 times the volume 95% ethanol, stand overnight at 4°C, centrifuge at 4000r / min for 15 minutes to get the precipitate, wash the precipitate 2-3 times with 75% ethanol, absolute ethanol, ether, and acetone, centrifuge at 4000r / min The precipitate obtained in 15 minutes was dissolved in distilled water to make it fully reconstituted. The Sevag method was used to remove protein. Repeatedly until no precipit...

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PUM

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Abstract

Cudrania tricuspidata polysaccharide is extracted at 70deg.C by: immersing raw materials for 6-8 hours, centrifugalizing, filtrating, incorporating filtrated liquids, passing them through macro-porous adsorbing resin-3520 with pH=4, decompressed concentrating, alcohol separating out, deproteinizing by Sevag method, dialyzing to pass gel column DEAE-Sephadex A-50, cleansing with water and NaCl solution, freezing dialyzed liquid and drying to obtain the products.

Description

Technical field: [0001] The invention relates to a method for extracting plant polysaccharides, specifically a method for extracting polysaccharides from a few roots. Background technique: [0002] Polysaccharide compounds can participate in the transmission and perception of information between immune cells, cell transformation, division and regeneration activities, etc., have the function of immune regulation; have anti-tumor, anti-virus, anti-aging, anti-coagulation, and anti-radiation damage effects ; It has the effect of lowering blood sugar, blood lipids and cholesterol, and anti-atherosclerosis; protecting the gastrointestinal tract, having the effect of anti-ulcer; having the effect of preventing liver damage; some mucopolysaccharides can adjust the concentration of anions in the body and regulate the production of collagen fibers , To prevent the absorption of heavy metal ions in the digestive tract of animals. A large number of pharmacological and clinical studies have ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 陈靠山董国霞程军
Owner ZHONGAO SCI & TECH SHANDONG LUKANG GROUP
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