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Technology for extracting hyaluronic acid from squid eyes

A technology of hyaluronic acid and squid, which is applied in the field of hyaluronic acid extraction, to achieve the effect of mild conditions, labor-saving and simple ingredients

Inactive Publication Date: 2018-03-30
桂林华诺威生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Squid is an important marine catch and raw material for aquatic products in my country. The squid eye, which is the processing waste, accounts for about 2% of the squid's weight, and has not been well utilized at present. Compared with the eyes of fish, squid eyes contain more vitreous body. Can be used as an extraction resource for hyaluronic acid

Method used

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  • Technology for extracting hyaluronic acid from squid eyes

Examples

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Comparison scheme
Effect test

Embodiment 1

[0023] A process for extracting hyaluronic acid from squid eyes, specifically comprising the steps of:

[0024] 1) Wash and smash the eyes of fresh squid;

[0025] 2) Subcritical water extraction: put the processed raw materials in an extraction kettle, add sodium acetate equivalent to 0.010 parts by weight of raw materials, inject deoxygenated deionized water into the kettle, extract at a temperature of 120-130 ° C, and a pressure of 5-8 MPa. The extraction time is 20 minutes, the squid eyes are extracted, the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature, and the extract is obtained;

[0026] 3) Add an equal volume of chloroform and n-butanol mixture to the extract obtained in the upward step, with a volume ratio of chloroform:n-butanol=4:1, stir evenly, and let stand;

[0027] 4) Purification: After standing and stratifying, take the supernatant and pass it through a DEAE-Sephadex A-25 ion colum...

Embodiment 2

[0030] A process for extracting hyaluronic acid from squid eyes, specifically comprising the steps of:

[0031] 1) Wash and smash the eyes of fresh squid;

[0032] 2) Subcritical water extraction: put the processed raw materials in the extraction kettle, add sodium acetate equivalent to 0.012 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle, the extraction temperature is 120-130 ° C, the pressure is 5-8 MPa, The extraction time is 25 minutes, the squid eyes are extracted, the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature, and the extract is obtained;

[0033] 3) Add an equal volume of chloroform and n-butanol mixture to the extract obtained in the upward step, with a volume ratio of chloroform:n-butanol=4:1, stir evenly, and let stand;

[0034] 4) Purification: After standing and stratifying, take the supernatant and pass it through a DEAE-Sephadex A-25 ion ...

Embodiment 3

[0037] A process for extracting hyaluronic acid from squid eyes, specifically comprising the steps of:

[0038] 1) Wash and smash the eyes of fresh squid;

[0039] 2) Subcritical water extraction: put the processed raw materials in the extraction kettle, add sodium acetate equivalent to 0.015 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle, the extraction temperature is 120-130 ° C, the pressure is 5-8 MPa, The extraction time is 30 minutes, the squid eyes are extracted, the extraction is completed, the pressure is released, the extract flows into the collection tank and cooled to room temperature, and the extract is obtained;

[0040] 3) Add an equal volume of chloroform and n-butanol mixture to the extract obtained in the upward step, with a volume ratio of chloroform:n-butanol=4:1, stir evenly, and let stand;

[0041] 4) Purification: After standing and stratifying, take the supernatant and pass it through a DEAE-Sephadex A-25 ion ...

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Abstract

The invention discloses a technology for extracting hyaluronic acid from squid eyes. The technology comprises the following steps: 1) cleaning the fresh squid eyes and smashing; 2) subcritical water extraction: putting a processed raw material into an extraction kettle, adding sodium acetate, injecting deoxidized deionized water into the kettle, controlling an extraction temperature as 120 to 130DEG C, pressure as 5 to 8 MPa and extraction time as 20 to 30 minutes, extracting the squid eyes, releasing pressure after extraction finishes, making extract liquor flow into a collection tank to becooled to room temperature to obtain the extract liquor; 3) adding an isometric chloroform and n-butyl alcohol mixed solution into the extract liquor obtained in the last step, evenly stirring and standing, wherein a volume ratio of the chloroform to the n-butyl alcohol is equal to 4 to 1; 4) purification: taking supernatant after standing and layering to pass through a DEAE-SephadexA-25 ion column and eluting by a NaCl solution to obtain eluant; 5) drying: concentrating the eluant, dialyzing to remove salt and vacuum drying to obtain a finished product.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a technique for extracting hyaluronic acid from squid eyes. Background technique [0002] Hyaluronic acid (HA for short) is an acidic polymucopolysaccharide, which is composed of N-acetylglucosamine and glucuronic acid repeatedly and alternately linked by β-1,4 and β-1,3 glycosidic bonds. . Due to its unique properties of high molecular polysaccharides such as water retention and lubrication, as well as natural, non-allergic and good biocompatibility with human tissues, it is widely used in the fields of cosmetics, health food and medicine. [0003] At present, hyaluronic acid is extracted from animal tissues mainly using cockscombs, human umbilical cords, animal eyeballs, etc. as raw materials, or produced by bacterial fermentation. The extraction and refining of hyaluronic acid brings great difficulties, and also reduces the quality of hyaluronic acid, lim...

Claims

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Application Information

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IPC IPC(8): C08B37/08
CPCC08B37/0072
Inventor 韦忠明黄启斌
Owner 桂林华诺威生物科技有限公司
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