Process for extracting hyaluronic acid from cockscombs

Abstract

The invention discloses a process for extracting hyaluronic acid from cockscombs. The process comprises the following steps: (1) cleaning and crushing fresh cockscombs; (2) performing subcritical water extraction: putting the treated raw material into an extraction kettle, adding sodium acetate, injecting deoxygenated deionized water into the kettle, extracting the cockscombs at extraction temperature of 120 to 130 DEG C and pressure of 5 to 8 Mpa for 20 to 30 min, relieving the pressure at the end of the extraction, and putting extraction liquid into a collection tank for cooling to room temperature, thus obtaining extraction liquid; (3) performing suction filtration: adding kieselguhr which accounts for 0.010 to 0.012 of the weight of the extraction liquid into the extraction liquid obtained in the previous step, and performing suction filtration at 0.1 MPa, thus obtaining filtrate; (4) performing purification: putting the filtrate obtained in the previous step through a DEAE-Sephadex A-25 ion column, and eluting the filtrate with a NaCl solution, thus obtaining eluent; (5) performing drying: concentrating the eluent, dialyzing the eluent to remove salt, and performing vacuum drying to obtain a finished product.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a process for extracting hyaluronic acid from cockscombs. Background technique [0002] Hyaluronic acid (HA for short) is an acidic polymucopolysaccharide, which is composed of N-acetylglucosamine and glucuronic acid repeatedly and alternately linked by β-1,4 and β-1,3 glycosidic bonds. . Due to its unique properties of high molecular polysaccharides such as water retention and lubrication, as well as natural, non-allergic and good biocompatibility with human tissues, it is widely used in the fields of cosmetics, health food and medicine. [0003] At present, hyaluronic acid is extracted from animal tissues mainly using cockscombs, human umbilical cords, animal eyeballs, etc. as raw materials, or produced by bacterial fermentation. The extraction and refining of hyaluronic acid brings great difficulties, and also reduces the quality of hyaluronic acid, limit...

AI Technical Summary

Problems solved by technology

However, the quaternary ammonium salt method is easy to precipitate the protein together when precipitating HA, reducing the purity of HA, and the amount of quaternary ammonium salt is relatively large; the chloroform extraction method is not only complicated in operation, but also causes damage to the human body and the environment; Due to the high viscosity of the acid, the high-speed centrifugation method is difficult to completely separate the bacteria from the fermentation liquid, and it is difficult to apply to industrial production; the flocculation method and the quaternary ammonium salt method also have the problems of high production cost and incomplete removal of impurities; the diameter of the pores of the microporous membrane is between Under the continuous action of pressure, it will become larger and cannot completely retain the bacteria and protein in the fermentation broth

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