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Wheat seed germination period drought resistance property evaluation method

A technology of wheat seeds, germination period, applied in the direction of germination equipment, etc.

Inactive Publication Date: 2005-03-16
CROP BREED RESOURCES INST CHINESE ACAD OF AGRI SCI
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Problems solved by technology

However, taking wheat as the research object, there are few research reports on the comparative analysis of osmotic stress during the germination stage of wheat seeds.
In particular, it has not been reported that it is systematically proposed as a condition for evaluating and identifying the drought resistance of wheat seeds during germination.

Method used

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Embodiment

[0024] After fully mixing the seeds of the wheat to be tested, 800 grains were randomly selected, dried at 30°C-35°C, and cooled to room temperature. Dissolve 192 grams of polyethylene glycol-6000 (ie, PEG-6000) in 1000 ml of deionized water to prepare a -0.5 MPa PEG-6000 aqueous solution. Every 100 wheat seeds in the stress culture is a repetition, a total of four repetitions, put them into germination dishes with length × width × height = 10cm × 10cm × 5cm, add 10ml of prepared PEG-6000 aqueous solution, cover, and avoid Water evaporates, marked as T 1 , T 2 , T 3 and T 4 . The control culture is the same as the stress culture, the difference is that 10ml of deionized water is added to the germination dish, respectively marked as CK 1 、CK 2 、CK 3 and CK 4 .

[0025] Put the above-mentioned 8 germination dishes into the incubator, control the temperature at 20°C±2°C, and start investigating the number of germinated seeds after 168 hours. The results are shown in Tabl...

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Abstract

Disclosed is a method for evaluating the wheat seed drought resisting property during germination period by utilizing the method of hypertonic solution which comprises, conducting moisture forcing treatment to the wheat seeds using polyethylene glycol 6000 (PEG-6000), charging the seeds into germination dish, storing in cultivation case, culturing to eighth day and surveying number of germination seeds, taking culture by deionized water for comparison, calculating seed germination percentage and relative germination percentage respectively.

Description

technical field [0001] The invention relates to a method for evaluating the drought resistance of wheat seeds in germination stage. Background technique [0002] In most wheat producing areas in northern my country, drought is the main factor limiting yield, and wheat may suffer from drought stress throughout its life. After sowing, the germination of seeds will directly affect the number of basic seedlings. Therefore, knowing the drought resistance of wheat seeds during germination will help to choose the sowing amount according to the soil moisture, and lay a good foundation for obtaining the target size of the population. For the drought resistance identification of cereal crop seeds in the germination stage, hypertonic solution is used to simulate water stress. The more commonly used hypertonic solution stress agents include polyethylene glycol (PEG) and mannitol with different molecular weights, and sucrose is also used as a stress agent. reports. In practice, sucrose...

Claims

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Application Information

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IPC IPC(8): A01C1/02
Inventor 景蕊莲昌小平胡荣海
Owner CROP BREED RESOURCES INST CHINESE ACAD OF AGRI SCI
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