Relativity between gene 1A of arginine-vasopressin receptor and essential hypertension

An arginine vasopressin and gene technology, applied in the fields of molecular biology and medicine, can solve the reports that do not confirm the correlation between AVPR1A gene and essential hypertension, and do not confirm the relationship between AVPR1A gene SNP and essential hypertension. Relevant reports, etc.

Inactive Publication Date: 2005-08-31
FUDAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Although there have been many studies on the relationship between various gene polymorphisms and essential hypertension, there is no report on the correlation between AVPR1A gene and essential hypertension, and there is no confirmation that the SNP of AVPR1A gene is associated with essential hypertension sex reports

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] 1.1 Research object

[0067]Since blood pressure is a quantitative trait that is affected by multiple genes and is affected by various environmental factors, it is necessary to consider the issue of genetic heterogeneity when analyzing related genes. Selecting isolated populations with a relatively single genetic background as materials for the study of polygenic diseases will help reduce the impact of genetic heterogeneity. reduce. Selecting such a group, whether it is used for linkage disequilibrium analysis or selecting families for family analysis, will help to improve the detection sensitivity of hypertension susceptibility loci. Therefore, in this embodiment, the isolated population is selected as the research object (the isolated population has the advantages of relative consistency of genetic background and relatively small number of founders).

[0068] On the basis of informed consent, 324 individuals of Han nationality over the age of 50 were randomly collec...

Embodiment 2

[0087] Essential Hypertension Susceptibility Detection Kit

[0088] As described in Example 1, the C→T mutation at position 2408 in SEQ ID NO: 1 is closely related to hypertension. Therefore, based on this mutation, AVPR1A gene-specific primers can be designed to amplify and detect using the patient's DNA as a template.

[0089] name

sequence

concentration

sense primer

SEQ ID NO: 2

100pmol

antisense primer

SEQ ID NO: 3

100pmol

PCR reaction solution

Containing Taq Enzyme dNTP Magnesium Ion PCR Reaction Buffer

[0090] Take 3ml of blood from the male patient to be tested, and use a conventional method (or use a specific kit) to extract DNA from the blood. Dilute the PCR primers in the high blood pressure detection kit to 1μmol / μ, and use the extracted DNA as a template to perform a PCR reaction with the provided primers. After purification of PCR products, ABI-PRISM TM 377 DNA sequencer was used for two-way sequ...

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Abstract

A process for testing the susceptibility of primary hypertension includes detecting if there are variations in arginine-vasopressin receptor 1A gene AVPR1A, transcript and/or protein of an individual, and determining its high susceptibility if there are. Its reagent kit is also disclosed.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine. More specifically, it relates to the single nucleotide polymorphism (singlenucleotide polymorphism, SNP) of arginine vasopressin receptor 1A gene (arginine vasopressin receptor 1A, AVPR1A) and its correlation with essential hypertension. The present invention also relates to methods and kits for detecting these SNPs. Background technique [0002] Hypertension refers to a group of clinical syndromes with elevated systolic or diastolic blood pressure. There is an obvious causal relationship between the increase of blood pressure and the occurrence of coronary heart disease, renal dysfunction, hypertensive heart disease and hypertension complicated with stroke. The latest diagnostic criteria for hypertension are systolic blood pressure ≥ 19kpa (140mmHg) or diastolic blood pressure ≥ 12kpa (90mmHg), and those who meet one of them can be diagnosed as hypertension. [0003] Mos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/68
Inventor 金力黄薇姜正文王颖张晨辉李艳平王志敏肖君华卢大儒
Owner FUDAN UNIV
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