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Process for extracting tanshinone

An extraction method and technology of tanshinone, applied in the field of medicine, to achieve the effects of easy industrialization, increased column capacity, and reduced product cost

Active Publication Date: 2005-09-21
TIANJIN TASLY MORDEN TCM RESOURCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the method of extracting fat-soluble components in Salvia Miltiorrhiza in combination with ethanol and macroporous resin

Method used

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  • Process for extracting tanshinone
  • Process for extracting tanshinone
  • Process for extracting tanshinone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: the extraction method of tanshinone

[0043] Step 1, alcohol extraction (soaking method)

[0044] For the first extraction, take 1000g of Danshen raw medicinal materials to make decoction pieces, add an appropriate amount of ethanol with a concentration of 25%, and the amount of alcohol added is about 10 times that of the raw medicinal materials, soak at room temperature for 12 hours, adjust the concentration of ethanol to 65%, and heat and reflux for 2 hours. Filter to separate the medicinal residue and medicinal liquid, and set aside.

[0045] For the second extraction, take the Danshen medicinal residues obtained above, add 65% ethanol, the addition amount is 2.5 times the volume of the medicinal residues, heat and reflux for 1 hour, and obtain the medicinal liquid; combine the above first and second extractions obtained The medicinal liquid is filtered to remove solid impurities in the medicinal liquid, and is set aside.

[0046] Step 2, separation b...

Embodiment 2

[0051] Step 1, alcohol extraction (soaking method)

[0052] For the first extraction, 1000g of the raw medicinal material of Danshen was made into decoction pieces, an appropriate amount of methanol with a concentration of 20% was added, and the amount of alcohol added was about 5 times that of the raw medicinal material, soaked at room temperature for 10 hours, adjusted the concentration of methanol to 60%, and heated to reflux for 1 hour. Filter to separate the medicinal residue and medicinal liquid, and set aside.

[0053] For the second extraction, take the Danshen medicinal residue obtained above, add 60% methanol, the addition amount is 2 times the volume of the medicinal residue, heat and reflux for 0.5 hour, and obtain the medicinal liquid; combine the above first and second extractions obtained The medicinal liquid is filtered to remove solid impurities in the medicinal liquid, and is set aside.

[0054] Step 2, separation by column chromatography

[0055] ① Put the...

Embodiment 3

[0059] Step 1, alcohol extraction (soaking method)

[0060] For the first extraction, 1000g of Danshen raw medicinal materials were taken to make decoction pieces, and an appropriate amount of propanol with a concentration of 22% was added. hour, filter, separate the dregs and liquid medicine, and set aside.

[0061] For the second extraction, take the Danshen herb dregs obtained above, add 60% propanol, the amount added is 2 times the volume of the dregs, and heat to reflux for 0.5 hour to obtain the medicinal liquid; combine the above first and second extractions to obtain The liquid medicine, filter, remove the solid impurity in the liquid medicine, set aside.

[0062] Step 2, separation by column chromatography

[0063] ① Put the medicinal liquid obtained through alcohol extraction in step 1 on a D101 macroporous adsorption resin column;

[0064] ②Elute with 65% ethanol, discard the initial filtrate, collect the eluate, recover ethanol, and obtain extract I (containing ...

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Abstract

The invention relates to a method for extracting tanshinone which comprises the following two steps: first, alcohol extraction process: separating the extraction liquid and dregs with the danshen liposolubility content from danshen medicine materical crude slices or disintegrated danshen medicinal materials by bath method, hypersonic extraction or microwave extraction in condition of normal temperature or heating, and reserving, adding correct concentration alcohol into the dregs, inverse flow extracting with heating to get the extraction, which combined with the extraction get from the first extraction, removing the solid impurities by the methods of filtration, centrifugation or deposition, and reserving. Second, isolation method separation: adding the medicine liquid get from the said alcohol extraction process to a macroreticular resinous adsorbent column, gradient eluting by alcohol with a finite concentration respectively to get the extraction I (with cryptotanshinone and dihydrotanshinone I) and extraction II (with tanshinone IIA and tanshinone I).

Description

technical field [0001] The invention relates to the field of medicine, in particular to a method for extracting tanshinone. Background technique [0002] Danshen (Salivia miltiorrihiza Bunge) is a commonly used traditional Chinese medicine in my country. It has many clinical effects, such as analgesia and antipruritic. It has a certain curative effect on liver shrinkage for gynecological diseases and jaundice infectious hepatitis. Pharmacological efficacy in recent years has proved that Salvia miltiorrhiza also has the effect of improving coronary circulation, inhibiting inflammation and detumescence of thrombotic diseases, and has been marketed as a patented medicine. Tanshinone compounds are active ingredients in the soluble ingredients of salvia miltiorrhiza, and are index ingredients of various medicines containing salvia miltiorrhiza. [0003] At present, there are many reports on the extraction methods of tanshinone. Cao Mingcheng et al [E...

Claims

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Application Information

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IPC IPC(8): C07C45/78C07C49/675C07D307/77
Inventor 郑志刚杨悦武王双明陆文亮
Owner TIANJIN TASLY MORDEN TCM RESOURCES
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