Primer, probe series and method for multiple real time fluorescent RT-PCR detection of H5, H7 and H9 subtype bird flu

Abstract

This invention discloses an nucleotide priemr tested by multiple real-time fluorescence RT-PCR, probe sequence and its method suitable for high-pathogenicity fowl-influenza H5, H7, H9 hypotype, relating to the nucleotide fragment RT-PCR augmenting primer and its complementary sequence, and the parting testing method of fowl-influenza H5, H7, H9 hypotype .

Description

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AI Technical Summary

Benefits of technology

This new method uses powerful techniques like polymerase chain reaction (PC) for DNA analysis with fast response times compared to traditional methods such as gel electrophoretic or immunological assays. It achieves this through efficient amplifying and detecting gene segments from samples containing both viruses A/B/C). By doing these operations at once, there are more precise data than current technologies but less expensive because they only require multiple steps instead of just two.

Problems solved by technology

The technical problem addressed in this patents relates to developing a new way to quickly identify and accurately monitor major human respiratory illness threatening agents such as swine fever coronaria vims, severe hemorrhages associated with porcinera fowls, salmon sialitis virus, Newcastle Diseas To address these issues, the inventors developed several novel techniques called multiplex real -world fluorescein qistype immunology assays (MFA). These technologies aim to provide better tools for early diagnosis and prevention efforts against pandemics like Spanishfluand Novel Bird Arteries.

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