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Gene engineering vaccine for weaning pigling diarrhea and pig hydrops disease

A genetically engineered vaccine and technology for weaned piglet diarrhea, applied in gene therapy, blood diseases, extracellular fluid diseases, etc., to achieve good protection effect

Inactive Publication Date: 2005-10-19
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no PWD, ED genetic engineering vaccine products at home and abroad

Method used

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  • Gene engineering vaccine for weaning pigling diarrhea and pig hydrops disease

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Experimental program
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Effect test

Embodiment Construction

[0010] 1. Clone the immune protective antigen genes such as F4, F18 and Stx 2e by PCR method.

[0011] 2. The above-mentioned genes were concatenated into the expression vector pGEX-6P-1 by means of molecular biology methods, and successfully expressed.

[0012] 3. Carry out immune protection experiments in mice and pigs, and prove that the developed genetic engineering vaccine can effectively prevent PWD and ED.

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Abstract

The gene engineering vaccine for pigling weaning diarrhea (PWD) and pig edema disease (ED) belongs to the field of stockbreeding and veterinary technology. Primer containing specific cleavage site is first designed based on the faeG(F4), fedA(F18) and Stx 2e B subunit gene sequences; protective antigen gene is amplified through PCR process with PWD and ED colibucillus plasmid operate or genome DNA as template; said gene is directionally connected serially to the expressive plasmid pGEX-6P-1, used to transform host bacillus BL21 and IPTG inducing expressed; and the expression of the target gene is SDS-PAGE and Western-blot proved. The recombinant bacilli expressing the above-said three protective antigen genes are immunized to mouse and pig via different ways so as to evaluate the immunogenicity and safety. Test shows that the vaccine may be used to prevent PWD and ED effectively.

Description

technical field [0001] The invention relates to the development of a vaccine for weaned piglet diarrhea and swine edema disease, belonging to animal husbandry and veterinary medicine [0002] technology field. Background technique [0003] Weaning piglet diarrhea [postweaning Escherichia coli diarrhea, PWD] and porcine edema disease [edema disease, ED] are important pathogens of weaning piglet death, the peak of weaning piglet death caused by Escherichia coli is usually 5 to 10 weeks old, PWD and ED are easy I feel close in age. Piglets with PWD pull light yellow or gray-white thin feces, which usually last for 1 week or more. The piglets gradually lose weight, become depressed, lose appetite, have rough coat, and some piglets die; ED is characterized by ataxia, facial or general edema , characterized by a high mortality rate. Specific serotypes of Escherichia coli are the main pathogens causing PWD and ED. Escherichia coli that cause PWD and ED mainly produce two types ...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61P1/12A61P7/10
Inventor 高崧刘秀梵施慧杨健美曹传闺
Owner YANGZHOU UNIV
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