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Industrial prodn. of Huan-Cao dendrobe (dendrobium loddigessi Rolfe) technique and culturing method therefor

A technology of Dendrobium eccentricum and production technology, which is applied in horticultural methods, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems that restrict the industrialization and large-scale development of authentic medicinal material Dendrobium eccentricum, and shorten the time of tissue culture. Time and cost, solution to authenticity, effect of increased propagation speed

Inactive Publication Date: 2005-11-09
吉仁堂药业有限公司
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AI Technical Summary

Problems solved by technology

Seriously restricting the industrialization and large-scale development of the authentic medicinal material Dendrobium annua

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: rapid propagation technology of Herba Dendrobii seedlings

[0027] After the mature capsules of the wild Dendrobium candidum are harvested, they are placed in a clean petri dish, and after they crack naturally, the seeds are put into a clean beaker and sealed, and stored in a refrigerator.

[0028] Put fresh uncracked fruit or stored seeds through a certain disinfection procedure, sow 1 / 2 MS basic medium + NAA (a-naphthalene acetic acid) 0.5 mg / L + banana taro juice 20% (V / V) + In the medium of agar 5g / L, carry out the seed germination culture, the culture temperature is 24 ℃, the light is 9 hours a day, the light intensity is 2500lx, the seed embryo starts to germinate in about 12 days, the seed embryo expands in 20-30 days, and gradually germinates to form a green protocorm;

[0029] Transfer the protocorm to 1 / 2 MS basic medium + BA (6-benzyl adenine) 0.5 mg / L + NAA (a-naphthalene acetic acid) 0.2 mg / L + canna juice 20% (V / V) + agar 5g / L medium for p...

Embodiment 2

[0031] Embodiment 2: the seedling hardening technique of tissue culture seedling of Herba Dendrobii

[0032] Seedling hardening substrate is small bluestone (3cm-5cm) + humus soil + moss, ratio: 5:3:2 (thickness);

[0033] Seedling hardening method: use a 1.5m×22m seedbed, spread 5cm of small bluestone, 3cm of humus soil, and 2cm of moss on the seedbed. After all are sterilized, take out the seedlings in the culture bottle and wash them, and then sterilize them in Duojingling After 5-10 minutes, the seedlings are graded and hardened again, and 3-5 plants are taken as a clump, and the roots are planted in the humus soil under the moss, and then it is advisable to pour root-fixing water once.

[0034] Conditions for hardening seedlings: Generally, warm sheds are used for hardening seedlings, and the temperature in the sheds is relatively controlled. In order to adapt to the conditions for transplanting in the future, constant temperature conditions should not be used for the tem...

Embodiment 3

[0035] Embodiment 3: Dendrobium candidum transplanting technology

[0036] Transplanting medium: bluestone ranging from 3cm to 8cm + humus soil 3cm + moss, the thickness ratio is: 8cm: 3cm: 2cm;

[0037] Transplanting conditions: ① Outside the shed, rocky mountainous areas with better shading, generally below the mountainside, in addition to retaining wild moss conditions, artificial planting seedbeds can be used. Spread a 1cm wide cultivation bed in the open space, with 40cm intervals. The base can be selected at an altitude below 1200m. Shade cloth is used for shading, the temperature is mainly in nature, the humidity is between 60%-85%, the shading degree reaches 60%, and the light is about 2000-3000lx.

[0038] ② Cultivation in sheds, using plastic greenhouses, using seedbeds or cultivation, the seedbed size is 1.5m×22m. The scattered light in the shed reaches more than 40%, about 1500-2800lx. The temperature can be between 5°C and 35°C, and the humidity should not be ...

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Abstract

An industrial culture technique for Huancao dendrobium is disclosed. Its embryo inducing process, the culture medium for making seedling strong and rooting and the culture medium for differentiating and reproducing protocorm are switabl for culturing other varieties of dendrobium. Its advantages are short period, high yield, and high contents of saccharide and trace elements in dendribium.

Description

technical field [0001] The patent application of the present invention relates to the technical field of cultivation of traditional Chinese medicinal materials, in particular to an industrial production technology and a cultivation method of medicinal Dendrobium chinensis Dendrobium. Background technique [0002] Dendrobium Dendrobium. Alias: yellow grass, the second largest genus of Orchidaceae, is a national endangered medicinal plant. Xingyi City, Guizhou Province is a place rich in dendrobium in history. It was called Huangcaoba in the past. Today, the central town of Xingyi is still called Huangcaoba Town. Due to the high medicinal value of Dendrobium, Dendrobium annulus is one of the five species of Dendrobium recorded in the Chinese Pharmacopoeia, and it is also widely used by the people. Dendrobium resources are basically on the verge of extinction. The country has listed this precious Chinese medicinal material as an endangered medicinal plant for protection, and ...

Claims

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Application Information

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IPC IPC(8): A01G7/00
Inventor 金家兴毛秀华
Owner 吉仁堂药业有限公司
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