Reagent composition for detecting beta-lactamase, detection kit and detection method

A detection kit, a technology for lactamase, applied in the directions of biochemical equipment and methods, measuring devices, biological material analysis, etc., can solve the problem of not revealing the possibility of β-lactamase and not disclosing β-lactamase inhibitors. Issues such as combination

Inactive Publication Date: 2006-02-01
昭和药品化工股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this international publication pamphlet does not disclose the combination with a specific β-lactamase inhibitor, nor does it suggest the possibility of detecting other types of β-lactamases other than ESBL.

Method used

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  • Reagent composition for detecting beta-lactamase, detection kit and detection method
  • Reagent composition for detecting beta-lactamase, detection kit and detection method
  • Reagent composition for detecting beta-lactamase, detection kit and detection method

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Embodiment

[0096] 1. Preparation of a solution comprising a β-lactamase detection reagent composition

[0097] 1.25 mg of the β-lactamase detection substrate and 1.25 mg of the β-lactamase inhibitor shown in the following Table 1 were dissolved in a mixed solvent of 0.1 mL of dimethyl sulfoxide and 0.9 mL of phosphate buffer, A solution comprising a β-lactamase detection reagent composition is prepared.

[0098] Reagent number

β-lactamase detection substrate

β-lactamase inhibitors

1

Nitrocephalin

none

2

Nitrocephalin

AZT, EDTA

3

Nitrocephalin

CVA, EDTA

4

Nitrocephalin

AZT, CVA

5

HMRZ compound

AZT, EDTA

6

HMRZ compound

AZT, CVA, EDTA

7

HMRZ compound

none

8

Nitrocephalin

AZT

9

Nitrocephalin

CVA

10

Nitrocephalin

AZT, CVA

11

HMRZ compound

AZT

12

HMRZ compound

AZT, CVA

...

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Abstract

It is intended to provide a reagent composition for detecting beta-lactamase which contains 3-[2,4-dinitrostyryl]-7-(2-thienylacetamido)-3-cephem-4-carboxylic acid or 7-[2-(2-aminothiazol-4-yl)-2-(1-carboxy-1-methylethoxyimino)acetamido]-3-(2,4-dinitrostyryl)-3-cephem-4-carboxylic acid as a substrate for detecting beta-lactamase together with at least one beta-lactamase inhibitor selected from among clavulanic acid, aztreonam, ethylenediaminetetraacetic acid and cloxacillin and by which beta-lactamase can be quickly and easily identified at a high sensitivity. Moreover, a detection kit containing the above detection reagent composition and a method of detecting beta-lactamase comprising contacting a liquid specimen containing the subject to be analyzed with the above composition are provided.

Description

technical field [0001] The invention relates to a beta-lactamase detection reagent composition, a beta-lactamase detection kit and a beta-lactamase detection method. Background technique [0002] β-lactamases are a group of enzymes that hydrolyze the intramolecular β-lactam ring of β-lactam antibacterial agents, and are divided into A, B, C and D according to the homology of the amino acid sequence of the enzyme protein. Extended-spectrum β-lactamase (ESBL) is an enzyme that decomposes a wider range of substrates (β-lactam antibacterials) than conventional A-type β-lactamases. These β-lactamases inactivate the antibacterial activity of β-lactam antibacterial agents, and among them, ESBL has been regarded as a problem as a pathogen of nosocomial infections. Even if a β-lactam antibacterial agent is administered to bacteria showing resistance to the β-lactam antibacterial agent, not only the therapeutic effect cannot be obtained, but it may even cause the spread of new resist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/34
CPCG01N2333/986C12Q1/34
Inventor 村田敦
Owner 昭和药品化工股份有限公司
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