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Anti-cd33 antibodies and method for treatment of acute myeloid leukemia using the same

A technology of antibody and identity, applied in the field of antibody binding to CD33

Inactive Publication Date: 2006-06-28
IMMUNOGEN INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] [12] Although the results discussed above indicate that immunoconjugates containing anti-CD33 antibodies and cytotoxic drugs can be successfully used in the treatment of AML, there is still a need for immunoconjugates that are both safe and effective

Method used

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  • Anti-cd33 antibodies and method for treatment of acute myeloid leukemia using the same
  • Anti-cd33 antibodies and method for treatment of acute myeloid leukemia using the same
  • Anti-cd33 antibodies and method for treatment of acute myeloid leukemia using the same

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preparation example Construction

[0199] [164] 1.3. Preparation and sequencing of heavy and light chain samples

[0200] [165] The heavy and light chains of the muMy9-6 antibody were separated by SDS-PAGE under reducing conditions. Reduced and denatured antibodies were run on 12% Tris-glycine gels (Novex, San Diego, CA). After electrophoresis, gels were spotted onto Immobilonpsq membranes using CAPS / MeOH buffer. After transfer, membranes were stained with Ponseau S. The bands corresponding to the light and heavy chains were excised for protein sequencing.

[0201] [166] The light chains of the antibodies were sequenced directly from the membrane on an ABI 494 Procise sequencer by automated Edman degradation chemistry.

[0202] [167] The N-terminus of the heavy chain was blocked, so the protein was digested in situ with trypsin according to Gharahdaghi et al. (1996). The digested mixture was then analyzed by MALDI-TOF mass spectrometry on a Kratos Kompact SEQ device. Selected peptides were subjected to MS / ...

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Abstract

The present invention relates to antibodies that bind CD33. More particularly, the invention relates to anti-CD33 antibodies, fragments and homologues of said antibodies, humanized and resurfaced versions of said antibodies, functional equivalents and improved versions of said antibodies, immunoconjugates and compositions comprising said antibodies, and the uses of same in diagnostic, research and therapeutic applications. The invention also relates to a polynucleotide encoding the antibodies, vectors comprising the polynucleotides, host cells transformed with polynucleotides and methods of producing the antibodies.

Description

[0001] [01] This application claims the benefit of Provisional Application No. 60 / 424,332, filed November 7, 2002, the disclosure of which is incorporated herein by reference. technical field [0002] [02] The present invention relates to antibodies that bind CD33. More particularly, the present invention relates to anti-CD33 antibodies, fragments and homologues of said antibodies, humanized and resurfaced forms of said antibodies, functional equivalents and improved forms of said antibodies, containing said antibodies Immunoconjugates and compositions, and their applications in diagnosis, research, and therapy. [0003] [03] In another aspect, the present invention relates to a polynucleotide encoding the antibody, a vector containing the polynucleotide, a host cell transformed with the polynucleotide, and a method of producing the antibody. Background technique [0004] [04] Leukocyte differentiation antigen (leukocyte differentiation antigen) CD33 is a transmembrane glyc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/395A61KA61K39/40A61K39/42A61K39/44C07H21/04C07K16/28C07K16/46C12N5/06C12N5/16C12N15/00G01N33/574
CPCA61K2039/505C07K2317/24C07K2317/565A61K47/6849C07K16/2803A61P35/00A61P35/02A61P43/00A61K47/68033C07K16/28A61K39/395A61K39/3955C07K16/2896C07K2317/70C07K2317/56C07K2317/92
Inventor M·G·霍费D·塔瓦雷斯R·J·卢茨
Owner IMMUNOGEN INC