Method for preparing extractive of perilla for killing activity of pine wood nematode

Abstract

A process for preparing the extract of perilla with the activity to kill the pine nematode includes such steps as providing fresh perilla leaves, immersing in distilled water, vacuum concentrating, and extracting in organic solvent. Its advantages are high killing activity and no poison.

Description

Technical field: [0001] The invention relates to a method for extracting a material component with pine wood nematode killing activity from natural plant perilla leaves, specifically a purple perilla with pine wood nematode killing activity mainly composed of rosmarinic acid. The preparation method of Su extract. technical background: [0002] Pine wood nematode [Bursaphelenchus xylophilus (Steiner & Buhrer) Nickle] is a devastating disease of pine trees, which is mainly spread by Monochamus alternatus Hope and can harm a variety of Pinus species. At present, the disease is distributed in Japan, the United States, Canada, China, South Korea, Nigeria and other countries. At the end of the last century, the disease was introduced to Europe. A large area of ​​dead pine wood nematodes were found in the pine forest around Lisbon, the capital of Portugal. pine. Since the introduction of pine wood nematode in Japan in the early 20th century, although a lot of manpower and materia...

AI Technical Summary

Problems solved by technology

In addition to its traditional functions, research data in recent years have shown that perilla also has the functions of calming, regulating lipid metabolism, antibacterial, anticoagulant, antipyretic, regulating blood sugar, anti-tumor, anti-oxidation, anti-allergic, anti-inflammatory, and promoting bowel function. peristalsis, inhibiting the activity of certain enzymes, etc., but the current development and utilization of perilla is limited to Chinese herbal medicine

In the process of studying the biologically active components of perilla, we found that one of the extracts has a high killing effect on pine wood nematodes, and can be used as a natural plant-sourced control drug against pine wood nematodes, which can solve the current control problems. Pine wood nematode disease has no specific medicines specifically for pine wood nematodes, and the existing methods cause serious environmental pollution and the disadvantages of unsatisfactory effects

According to literature search, there is no report about the killing activity of perilla extract on pine wood nematode at home and abroad.