Preparation of fungi extract and application thereof in resisting HIV

An extract and fungus technology, applied to the field of preparation of endophytic fungal extracts, can solve the problems of toxic and side effects of chemotherapeutic drugs, easy generation of drug resistance, destruction of vegetation and human living environment, etc.

Inactive Publication Date: 2006-12-27
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are currently the following problems in the treatment of AIDS: on the one hand, the high variability of HIV makes it easy to develop drug resistance; on the other hand, chemotherapy drugs have strong toxic and side effects, and most of these drugs are expensive, and many AIDS patients in economically backward countries and regions cannot afford medical expenses, so they ca

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0019] 1. Fermentation of fungi

[0020] After activation of Epulorhiza sp. fungi from slant test tubes preserved at low temperature, they were transferred to a φ90mm plate containing PDA medium, cultured at a constant temperature of 25°C for 12 days, and holes were punched at the edge of the colonies to form Bacterial flakes, and put into liquid culture medium with small fragments for fermentation culture;

[0021] The medium composition of fungal liquid culture is: glucose 20 g / L, KH 2 PO 4 3 g / L, MgSO 4 1.5 g / L; natural product is wheat bran 30 g / L (boiled juice), culture medium pH 5.5. 500 mL Erlenmeyer bottle culture medium 200 mL, sterilized at 121°C for 30 minutes, inoculated with Epulorhiza sp. . Culture conditions: shaking speed 110 rpm; 25°C dark cultivation for 10 days to harvest.

[0022] 2. Obtaining of Fungal Fermentation Culture

[0023] After the fermentation and cultivation of fungi of the genus Epulorhiza (Epulorhiza sp.), filter through a 100-mesh ny...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

Disclosed is an applocation of an abstract of a plant endogenetic fungi fermentation product for antagonizing immunodeficiency virus(HIV). Said fungi is isolated from the roots of Orchidaceae medicinal herb Dendrobium nobile, and is EF1(Epulorhiza sp.); EF1s are fluid fermentation cultured to prepare an abstract of the fermentation product. The invention is provided with simplicity of fermentation art, short period of production, and mass industrialization production. The abstract of the fermentation product is obtained easily, and has a remarkable anti-HIV-1 activity, the acting site of which is after reverse transcription and before HIV RNA expression, with a regulation action of HIV-1 gene expression. An adding action is obtained by a combined use of the said fungi fermentation product and anti-HIV drug AZT, indicated by a drug combination test. The abstract of the fungi fermentation product has an important value in the development of novle anti-HIV drugs, and is hopefule to become a novel anti-HIV drug.

Description

technical field [0001] The invention belongs to the technical field of bioengineering. Specifically, it relates to a preparation technology of an endophytic fungus extract which has anti-human immunodeficiency virus (HIV-1) activity and can regulate HIV-1 gene expression. technical background [0002] Acquired Immunodeficiency Syndrome, also known as AIDS (AIDS), was first reported by the US Centers for Disease Control in 1981. In the mid-1980s, AIDS had developed into a global epidemic, and its pathogen was identified as Human Immunodeficiency Virus (Human Immunodeficiency Virus, HIV). Over the past two decades, research in the field of AIDS pathology and treatment has achieved certain results. In recent years, high-efficiency antiviral treatment has significantly reduced the morbidity and mortality of AIDS. However, there are currently the following problems in the treatment of AIDS: on the one hand, the high variability of HIV makes it easy to produce drug resistance; o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K36/06C12N1/14C12P1/02A61P31/18
Inventor 郭顺星蒋岩相子春陈晓梅王春兰肖培根
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap