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Active extract of a strictly anaerobic marine bacterium, and its preparing method and use

A technology of marine bacteria and extracts, applied in the direction of bacteria, antibacterial drugs, medical raw materials derived from bacteria, etc.

Inactive Publication Date: 2007-01-10
DALIAN JIAOTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there has been no report on the isolation and extraction of pharmacologically active secondary metabolites from sulfate-reducing bacteria.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1. liquid culture medium is: KH 2 PO 4 0.5g; NH 4 Cl 1.0g; Na 2 SO 4 1.0g; MgSO 4 ·7H 2 O 2.0g; 70% (weight) sodium lactate 5.0g; yeast extract 1.0g; CaCl 2 2H 2 O 0.1g; FeSO 4 ·7H 2 O 0.5g; sodium thioglycolate 0.1g; vitamin C 0.1g; aged seawater 1000mL.

[0040] A. Seed culture: Fill the liquid medium into the serum bottle, leave a 2cm-high gap in the bottle, tighten the serum bottle cap slightly to facilitate ventilation, and sterilize in a high-pressure sterilizer at 121°C and 0.105mpa for 20min; after cooling Inoculate the marine strain DM18, fill it up with sterile medium, and tighten the bottle cap tightly; culture the serum bottle at a constant temperature of 30°C for 5 days;

[0041] B. Expanded cultivation: inoculate with 5-10% seed culture solution by volume, use a thick glass bottle with a cover as a culture container, adopt a similar seed culture process to cultivate a large amount of marine bacterial strain DM18, and cultivate it at ...

Embodiment 2

[0044] Embodiment 2. liquid culture medium is: KH 2 PO 4 0.5g; NH 4 Cl 1.0g; Na 2 SO 4 1.0g; MgSO 4 ·7H 2 O2.0g; 70% (weight) sodium lactate 5.0g; Yeast extract 1.0g; CaCl 2 2H 2 O 0.1g; FeSO 4 ·7H 2 O0.5g; Sodium Thioglycolic Acid 0.1g; Vitamin C 0.1g; Artificial Seawater 1000mL; 2 SO 4 4.0g, KCl 0.7g, NaHCO 3 0.20g, KBr 0.10g, H 3 BO 3 0.03g, NaF 0.003g, 53mL1.0mol / L MgCl 2 Solution, 10mL1.0mol / l CaCl 2 solution, 0.90ml 0.1mol / L SrO 2 Solution, distilled water 1000ml, pH adjusted to 7.2 ~ 7.4.

[0045] A. Seed culture: Fill the liquid medium into the serum bottle, leave a 2cm-high gap in the bottle, tighten the serum bottle cap slightly to facilitate ventilation, and sterilize in a high-pressure sterilizer at 121°C and 0.105mpa for 20min; after cooling Inoculate the marine strain DM18, fill it up with sterile medium, and tighten the bottle cap tightly; keep the serum bottle at a constant temperature of 26°C for 7 days;

[0046] B. Expanded cultivation:...

Embodiment 3

[0049] Embodiment 3. liquid culture medium is: KH 2 PO 4 0.5g; NH 4 Cl 1.0g; Na 2 SO 4 1.0g; MgSO 4 ·7H 2 O2.0g; 70% (weight) sodium lactate 5.0g; Yeast extract 1.0g; CaCl 2 2H 2 O 0.1g; FeSO 4 ·7H 2 O 0.5g; sodium thioglycolate 0.1g; vitamin C 0.1g; aged seawater 1000mL.

[0050] A. Seed culture: Fill the liquid medium into the serum bottle, leave a 2cm-high gap in the bottle, tighten the serum bottle cap slightly to facilitate ventilation, and sterilize in a high-pressure sterilizer at 121°C and 0.105mpa for 20min; after cooling Inoculate the marine strain DM18, fill it up with sterile medium, and screw the bottle cap tightly; keep the serum bottle at a constant temperature of 28°C for 6 days;

[0051] B. Expanded cultivation: inoculate with 5-10% seed culture solution by volume, use a thick glass bottle with a cover as a culture container, adopt a similar seed culture process to cultivate a large number of marine bacterial strain DM18 bacteria liquid, and cultiv...

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Abstract

The present invention belongs to the field of biological technology, and relates to a kind of marine bacterium with strict araerobic activity, active extract of said bacterial strain culture and its preparation method, and the application value of said extract in the preparation of antibacterial medicine. Said invention provides a marine bacterial strain DM 18 obtained from the Huanghai sea of China by means of separation process, it is defined as culture fluid of Desulfovibrio desulfuricans. Its ethyl acetate extraction phase and n-butyl alcohol extraction phase are undergone the processes of silica-gel column chromatography and ceversal high-pressure liquid-phase separation so as to obtain two oil-like substances of effective component which have resistance for hay bacillus and staphylococcus aureus, and have potential application for preparing antibacterial medicine.

Description

technical field [0001] The invention relates to a strain of strictly anaerobic active marine bacteria, in particular to the active extract of the marine bacterial strain culture, its preparation method and the use of the extract in antibacterial drugs. Background technique [0002] The development of new drugs derived from marine microorganisms is a worldwide research hotspot in the 21st century. It has attracted widespread attention due to its characteristics of new strain sources, production of new structural active substances, overcoming traditional antibiotic resistance, and realizing resource sustainability. . At present, many novel antibacterial antibiotics, antifungal antibiotics, antiviral antibiotics, antitumor antibiotics, and some anti-inflammatory and analgesic active substances, enzyme inhibitors, etc. have been isolated from various marine microorganisms. There is a class of strictly anaerobic microorganisms—sulfate-reducing bacteria in the ocean. These microo...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61P31/04C12N1/20C12P1/04
Inventor 穆军焦炳华梁占国吕红丽李彦生韦华生周荣丽
Owner DALIAN JIAOTONG UNIVERSITY
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