Active extract of a strictly anaerobic marine bacterium, and its preparing method and use
A technology of marine bacteria and extracts, applied in the direction of bacteria, antibacterial drugs, medical raw materials derived from bacteria, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0039] Embodiment 1. liquid culture medium is: KH 2 PO 4 0.5g; NH 4 Cl 1.0g; Na 2 SO 4 1.0g; MgSO 4 ·7H 2 O 2.0g; 70% (weight) sodium lactate 5.0g; yeast extract 1.0g; CaCl 2 2H 2 O 0.1g; FeSO 4 ·7H 2 O 0.5g; sodium thioglycolate 0.1g; vitamin C 0.1g; aged seawater 1000mL.
[0040] A. Seed culture: Fill the liquid medium into the serum bottle, leave a 2cm-high gap in the bottle, tighten the serum bottle cap slightly to facilitate ventilation, and sterilize in a high-pressure sterilizer at 121°C and 0.105mpa for 20min; after cooling Inoculate the marine strain DM18, fill it up with sterile medium, and tighten the bottle cap tightly; culture the serum bottle at a constant temperature of 30°C for 5 days;
[0041] B. Expanded cultivation: inoculate with 5-10% seed culture solution by volume, use a thick glass bottle with a cover as a culture container, adopt a similar seed culture process to cultivate a large amount of marine bacterial strain DM18, and cultivate it at ...
Embodiment 2
[0044] Embodiment 2. liquid culture medium is: KH 2 PO 4 0.5g; NH 4 Cl 1.0g; Na 2 SO 4 1.0g; MgSO 4 ·7H 2 O2.0g; 70% (weight) sodium lactate 5.0g; Yeast extract 1.0g; CaCl 2 2H 2 O 0.1g; FeSO 4 ·7H 2 O0.5g; Sodium Thioglycolic Acid 0.1g; Vitamin C 0.1g; Artificial Seawater 1000mL; 2 SO 4 4.0g, KCl 0.7g, NaHCO 3 0.20g, KBr 0.10g, H 3 BO 3 0.03g, NaF 0.003g, 53mL1.0mol / L MgCl 2 Solution, 10mL1.0mol / l CaCl 2 solution, 0.90ml 0.1mol / L SrO 2 Solution, distilled water 1000ml, pH adjusted to 7.2 ~ 7.4.
[0045] A. Seed culture: Fill the liquid medium into the serum bottle, leave a 2cm-high gap in the bottle, tighten the serum bottle cap slightly to facilitate ventilation, and sterilize in a high-pressure sterilizer at 121°C and 0.105mpa for 20min; after cooling Inoculate the marine strain DM18, fill it up with sterile medium, and tighten the bottle cap tightly; keep the serum bottle at a constant temperature of 26°C for 7 days;
[0046] B. Expanded cultivation:...
Embodiment 3
[0049] Embodiment 3. liquid culture medium is: KH 2 PO 4 0.5g; NH 4 Cl 1.0g; Na 2 SO 4 1.0g; MgSO 4 ·7H 2 O2.0g; 70% (weight) sodium lactate 5.0g; Yeast extract 1.0g; CaCl 2 2H 2 O 0.1g; FeSO 4 ·7H 2 O 0.5g; sodium thioglycolate 0.1g; vitamin C 0.1g; aged seawater 1000mL.
[0050] A. Seed culture: Fill the liquid medium into the serum bottle, leave a 2cm-high gap in the bottle, tighten the serum bottle cap slightly to facilitate ventilation, and sterilize in a high-pressure sterilizer at 121°C and 0.105mpa for 20min; after cooling Inoculate the marine strain DM18, fill it up with sterile medium, and screw the bottle cap tightly; keep the serum bottle at a constant temperature of 28°C for 6 days;
[0051] B. Expanded cultivation: inoculate with 5-10% seed culture solution by volume, use a thick glass bottle with a cover as a culture container, adopt a similar seed culture process to cultivate a large number of marine bacterial strain DM18 bacteria liquid, and cultiv...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com