Serum starch enzyme reagent kit and preparation method thereof

Abstract

A serum amylase kit is prepared as containing NaCI content of 70mmol / L, CaCI2 content of 1.0mmol / L and HEPES content of 50mmol / L in buffer liquid; containing EPS-GT content of 3.5mmol / L in substrate liquid; containing a-AMY content of 60U / ml and human serum albumin content of 20g / L in enzyme storing liquid; applying temperature of 37deg.c, buffer liquid pH of 7.15 and enzyme content of 7.1U / ml as testing conditions of kit when kit is used to carry out test.

Description

technical field [0001] The invention belongs to preparations and methods for measuring blood characteristics in vivo, in particular to serum amylase kits and preparation methods Background technique [0002] As early as 1929, Elman established the value of serum amylase (Amy) in the diagnosis of acute pancreatitis. There are four types of methods for measuring total activity. The first type is used to determine the consumption of substrate starch: by viscosity method, turbidity method, and iodine-starch method. These methods have poor accuracy and low sensitivity; the second type is sugar production. The third method is the chromogen substrate decomposition method, the dye is combined with the insoluble starch into the chromogen substrate (commonly known as dyed starch), and the dye is freed with the degradation of the starch under the catalysis of Amy, and the content of the dye is measured. Such methods require special equipment and are not widely used. The fourth type i...

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Problems solved by technology

[0005] Because the dissociation of 4-nitrophenol (4-NP) increases with the increase of temperature, but the current test temperature is 30°C, which is relatively low, so it affects the catalytic degradation reaction of α-Amy, with long lag time and narrow linear range , not suitable for routine analysis

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