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Gene sequence correlated with cordycepin biological synthesis

A biosynthesis and cordycepin technology, applied in the field of bioengineering, can solve the problems of unsatisfactory incremental effect and long cycle, and achieve the effect of increasing the content and promoting the expression of synthetase

Active Publication Date: 2007-03-14
JONJEE HI TECH INDAL & COMML HLDG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its shortcoming is that the conventional traditional method mentioned above has a long cycle and unsatisfactory incremental effect

Method used

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  • Gene sequence correlated with cordycepin biological synthesis
  • Gene sequence correlated with cordycepin biological synthesis
  • Gene sequence correlated with cordycepin biological synthesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0097] Specific implementation plan

[0098] Preparation method of cordycepin biosynthesis related genes of the present invention

[0099] (1) Screening of mutants

[0100] Using gene frameshift mutagens, the wild-type Cordyceps fungus is mutagenized to obtain a strain with high cordycepin expression.

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Abstract

The present invention relates to cordycepin biosynthesis relevant gene and its application. Seven cordycepin biosynthesis relevant genes are cloned by means of suppression and subduction hybridizing (SSH) technology. They are named separately as chcs1, chcs2, chcs3, chcs4, chcs5, chcs6 and chcs7 and have cDNA sequence lengths of 760bp, 774bp, 713bp, 823bp, 746bp, 763bp and 890bp separately. Through constituting high efficiency plant expression converting vector containing the said genes, it is possible to increase the copy quantity of biosynthesis gene inside thallus and to raise the content of cordycepin aweto expresses for meeting the requirement in developing medicine.

Description

[0001] The technical field [0002] The present invention relates to a secondary metabolite biosynthesis gene of Cordyceps in the field of bioengineering, in particular to a gene related to cordycepin biosynthesis. Background technique [0003] Cordyceps fungi are an important class of entomogenous fungi. Cordycepin is one of the important active ingredients of Cordycepssinensis and Cordyceps militaris. It has unique effects in antibacterial, antiviral, antitumor, insecticidal and other aspects. It is a kind of highly commercial Develop valuable nucleoside antibiotics. More than 100 articles have been published abroad describing the research on anti-tumor, anti-virus, anti-bacterial and extraction and purification of cordycepin. [0004] In 1951, American Cuningham and others first purified a substance from Cordyceps militaris and named it cordycepin. It was identified as 3′-deoxyadenosine (3′-deoxyadenosine). The molecular formula is C 10 H 13 N 5 O 3 , Molecular weight 251 Dalton...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/31C12N15/82C12N1/15C12P19/40
Inventor 叶小舟吴继明莫红丽邹爱国
Owner JONJEE HI TECH INDAL & COMML HLDG
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