Method for inspecting lucid ganoderma product quality
A technology of product quality and Ganoderma lucidum, which is applied in the field of detection of Ganoderma lucidum product quality, can solve the problems of low sensitivity, cumbersome detection methods, and long cycle, and achieve the effects of high sensitivity, high detection sensitivity, and easy operation
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[0040] The content of Ganoderma lucidum polysaccharide peptide in Shuangling Guben oral preparation was determined by using bovine serum albumin as the reference substance.
[0041]Preparation of peptide reference substance solution: take bovine serum albumin reference substance and add water to make a solution containing 0.3mg per 1ml.
[0042] Prepare the following solutions separately:
[0043] ①The solution of the test product: Method A: Take 1g of this product, weigh it accurately, add water to dissolve it to make a solution containing 0.6mg per 1ml, filter it, and set aside; Method B: Take 1g of the test product, add water to make a solution containing 0.6mg per 1ml 10 mg solution in 60°C water bath for 10 minutes, ultrasonic for 10 minutes, let cool, filter, take 4ml of the filtrate, add 12ml of absolute ethanol, stir, centrifuge at 10000rpm for 10 minutes, discard the supernatant, add water to dissolve the precipitate and constant volume to 10ml, that is.
[0044] ②B...
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